本研究以實驗室純種培養之藍綠藻(Microcystis aeruginosa)及綠藻(Chodatella sp.)作為藻類懸浮液，模擬優養化之水庫水，並以次氯酸鈉及臭氧進行氧化實驗，比較不同氧化劑量下，藻類水溶性有機質之變化情形。
為了得知藻類Soluble EPS之背景資訊，先對未氧化之M. aeruginosa及Chodatella sp.含藻懸浮液進行離心萃取的動作，並進行螢光激發與發射陣列之(Fluorescence Excitation-Emission Matrixs, EEMs)螢光分析、分子量分佈(high Performance Size Exclusion Chromatography, HPSEC)、蛋白質與碳水化合物之含量、非揮發溶解性有機碳及UV254之量測，結果顯示未氧化時Chodatella sp.與M. aeruginosa的Soluble EPS在分子群的分佈比例上皆以小分群(< 1 kDa)為主，其中分子群的訊號上M. aeruginosa相較於Chodatella sp.在大分子( > 10 kDa)及小分子群( < 1 kDa)佔有優勢。此外，經螢光特性分析顯示，兩種藻類皆以類芳香蛋白為主，而平均螢光強度部份，諸如類微生物代謝物質、類黃酸及類芳香蛋白，則以M. aeruginosa之訊號較強。根據螢光EEM的圖譜顯示，M. aeruginosa之類微生物代謝物質螢光訊號較Chodatella sp.強，這也顯示前者的水溶性胞外聚合物(Soluble EPS)釋出量較多。
氧化後的結果指出，兩種氧化劑皆會對藻類的Soluble EPS螢光特性產生變化，由芳香蛋白轉換到類黃酸，以M. aeruginosa為例，該現象分別出現在氯量0.39 mg/L及臭氧量0.12 mg/L時，對Chodatella sp.而言，則出現在加氯量7.7 mg/L時，臭氧的部份，在0.17 mg/L，由此劑量之差異，可知Chodatella sp.之Soluble EPS較具有抵抗氧化所造成的特性改質現象。
氧化後之分子量分佈方面，M. aeruginosa之大分子群在低臭氧劑量時即有強度增加的現象，而Chodatella sp.受低劑量臭氧氧化時並沒有觀察到。

In this research, algae suspensions, containing cyanobacteria (Microcystis aeruginosa) and green algae (Chodatella sp.), harvested from axenic cultures in the lab, are used to simulate the source water from eutrophic reservoirs. These suspensions were subjected to oxidation by either chlorine (NaOCl) or ozone. The effect of type of oxidants and dosages on the characteristics of extracellular polymeric substances (EPS) of algae was studied.
First, the soluble EPS from the original algal suspensions were extracted by centrifugation, and then characterized by NPDOC, UV254, protein and carbohydrate concentration, and also by fluorescence excitation-emission matrixs (EEMs) spectroscopy. The molecular size distribution of soluble EPS was analyzed by high performance size exclusion chromatography (HPSEC). The results show that, before oxidation, the molecular size distribution of soluble EPS of both algae species are dominated by smaller size group (＜ 1 KDa). Generally speaking, M. aeruginosa has higher EPS contents than that of Chodatella sp. However, the former has higher proportion of larger M.W. ( 10 KDa) and lower M.W. (1 KDa) compounds than that of the latter. According to EEM, the EPS of both algae species are dominated by aromatic protein-like. However, Chodatella sp. has higher proportion of soluble microbial by-product like, humic acid like, and fulvic acid like compounds than that of M. aeruginosa.
Both chlorine and ozone was found to change the fluorescence characteristic of soluble EPS of both algae. The major fluorophore type was changed from aromatic protein- like to fulvic acid-like after oxidation. For M. aeruginosa, that change occurred when chlorine and ozone dosages reached 0.39 and 0.12 mg/L, respectively; while the corresponding dosages for Chodatella sp were 7.7 and 0.17 mg/l, respectively. These results indicate that the soluble EPS of Chodatella sp were more insert to oxidation than that of M. aeruginosa. Concerning the change in molecular size distribution of soluble EPS, the results show that the shift of the distribution to larger M.W. group (＞10 kDa) after ozonation was occurred at low dosage for M. aeruginosa. However, similar phenomenon was not noticed for Chodatella sp.

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