光與物質間的強耦合現象會使得光與物質所構成的系統模態產生分裂，進而導致系統會處於一半光子模態，一半電子模態的現象，而對應到系統散射光譜的量測上會出現兩個峰值，也就是散射光譜峰值分裂的情形。在本研究的第一部分中，設計了一套簡易且快速的DNA修飾製程，將螢光分子侷限於奈米粒子與薄膜所構成的奈米共振腔中(粒子-薄膜共振腔)，而形成一耦合結構，並藉由量測此耦合結構的散射光譜，來觀察是否有因分子與共振腔互相耦合後所產生散射光譜峰值分裂的情形。為驗證實驗上觀測到的散射光譜峰值分裂是粒子-薄膜共振腔與螢光分子互相耦合所造成的結果，在實驗上透過比較了粒子-薄膜共振腔中內有無螢光分子之散射光譜量測結果，驗證了散射光譜上觀測到的峰值分裂是粒子-薄膜共振腔與螢光分子互相耦合所造成，且透過掃描電子顯微鏡（Scanning Electron Microscope，SEM）來觀測每一個量測的粒子-薄膜耦合結構是否為單顆粒子組成，去除了散射光譜峰值分裂的情形可能為粒子間互相影響所導致。接下來利用了多層膜修飾粒子-薄膜共振腔的方式來改變共振腔的共振模態，進而改變散射光譜分裂的峰值位置，來觀測耦合結構是否有抗交叉(Anti-crossing)的趨勢。最後藉由著量測耦合結構的螢光光譜來看是否會因粒子-薄膜共振腔與螢光分子互相耦合，所導致在耦合結構的螢光光譜上也觀察到有兩個峰值。而在理論驗證的方面，利用實驗上量測到的粒子-薄膜共振腔散射光譜的線寬、分子吸收光譜的線寬以及耦合結構散射光譜峰值分裂寬度來檢視本研究的是否有符合理論上的強耦合規範。
為了後續實務上的應用，將強耦合結構定位在特定的位置是一件很重要的工作。故在第二部分的研究利用了DNA序列末端硫鍵會與金鍵結的特性，搭配電子束微影技術(Electron Beam Lithography, EBL)可以將奈米尺度的金圓盤定位於特定位置上的方式，將金奈米粒子與特定位置的奈米金圓盤藉由DNA序列互相結合，來達成將強耦合結構定位的目的。而在實驗上雖然奈米金圓盤實際的尺寸與設計的尺寸約誤差10%，不過也說明了本研究使用的製程方法是可以成功將奈米金圓盤固定在特定的位置上。而粒子-圓盤共振腔與分子間強耦合實驗方面，延續第一部分所設計出的DNA修飾手法，將金奈米粒子藉由DNA序列互補的特性與奈米金圓盤結合，而在散射光譜無法看出共振腔內有無分子的差異，且在利用掃描式電子顯微鏡來側向拍攝粒子-圓盤共振腔結構上僅約略看出粒子接上金奈米圓盤的模樣，無法很確定的說明粒子是有接上金奈米圓盤的，而在散射光譜無法看到一些差異的問題上也有提出一些後續解決的方案。

Precisely position coupling between light and single molecule at room temperature is one of fundamental goals of building nanophotonic devices. Here we studied scattering spectrum of individual nanoparticle-film structure coupled to molecule, and form the coupling structure between light and matter. Then using the characteristics of thiol bond on DNA will be bond with gold, positioning the nanoparticle-disk structure in a specific area.
In the experimental results, the splitting can be obtained by comparing the results of scattering spectrum with or without molecules in the nanocavity. Moreover, by SEM observation, the comparison of the laser light before and after the illumination and layer by layer deposition can be more certain that the splitting result is due to the coupling of the molecules and the resonant cavity. But in theoretical calculations, it is approaching the strong coupling regime.
In the fabrication of the nanoparticle-disk structure, Au nanodisk has been successfully fabricated on the ITO/glass, and the actual diameter and height of Au nanodisk have been confirmed by SEM and AFM, respectively. After DNA functionalization and nanoparticle assembly, we can't see a clear image on the SEM to determine if the particles have successfully assembled on the Au nanodisk. In the results of the scattering spectrum, we cannot distinguish the difference in the scattering spectrum with or without molecules in the cavity. The experimental results and techniques in this aspect should be discussed and reviewed in the future.

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