本研究利用雷射雕刻技術，製備聚甲基丙烯酸甲酯(Poly-methyl-methacrylate, PMMA) 微流體晶片，並結合震盪晶片，運用於光交聯微小球與微膠囊之製備。研究策略是將光交聯溶液(UV-Polymer solution)與礦物油(Mineral oil)注入微流道中，透過震盪晶片所產生之超音波製備出光交聯微乳化球。經實驗結果顯示輸入電壓之大小與超音波震盪距離皆會影響所生成之光交聯微乳化球的粒徑大小，且經由365 nm之紫外光照射，光交聯微乳化球可固化形成光交聯微小球，其粒徑大小分佈在1.2 ~ 9.5 μm之間。應用以上的結果，本研究進一步透過微膠囊(Microcapsules)製備原理，在後端流道加入雙Y字型設計，將散佈於礦物油內之光交聯微小球包覆於褐藻酸鈉(Alginate)內，並藉由控制褐藻酸鈉(包覆相)與葵花油(剪切相)流速，成功製備出粒徑大小分佈在89.0 ~ 248.9 μm之間的褐藻酸鈉微膠囊。

This study managed to generate UV-photopolymerized microparticles using an ultrasonic vibration microfluidic chip coupled with external UV-crosslinking reaction. We have demonstrated that the sizes of UV-crosslinking microparticles were able to be controlled from 1.2 μm to 9.5 μm in diameter (with a variation of less than 10%) by altering the ultrasonic vibration distance and the voltage of power supply provided to transducer. Then by using hydrodynamic focusing effect, we design a double Y cross-junction microfluidic chip to generate microcapsules. By altering the wrap phase and sheath phase flow rate, the microcapsule size is controlled, the distribution of which is from 89.0 μm to 248.9 μm in diameter (with a variation of less than 10%). The proposed ultrasonic vibration microfluidic chip was easy to fabricate and capable of high throughput and low cost.

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