全球養殖蝦業就現階段而言，受到多種疾病的侵擾而蒙受極大的經濟損失，其中包含了新興疾病—急性肝胰腺壞死病(Acute hepatopancreatic necrosis disease，AHPND)。AHPND為新興細菌性蝦類疾病，其病原為帶有特殊質體pVA1之副溶血弧菌 (Vibrio parahaemolyticus，VP) 。儘管近年來有大量對於AHPND相關的研究，其中的分子致病機制以及AHPND病蝦中宿主的反應仍尚未被釐清。因此，透過次世代定序並結合生物資訊學的分析，觀察白蝦蝦胃中轉錄體的變化，希望進一步找出AHPND致病的關鍵因子或是其相關路徑。首先，以Pir毒素基因作為受到AHPND感染的指標，觀察白蝦於浸泡感染後的情況。接著將樣本抽取其總RNA進行次世代定序。在生物資訊分析上，以TSB培養基處理組作為控制組，挑出不同處理組中有差異表現量之基因 (DEGs) ，進一步利用ContigViews平台建構出致病株與非致病株處理的基因共表現網絡。從該網絡分析結果與先前實驗室所得數據，從本研究的結果推測AHPND會影響蝦胃免疫系統。而此平台能幫助學者更加了解副溶血弧菌與蝦之間關聯性的工具，並找出AHPND的關鍵因子加以預防。

Global shrimp aquaculture has had substantial production losses due to various diseases, including acute hepatopancreatic necrosis disease (AHPND), a recently emergent bacterial disease, caused by strains of Vibrio parahaemolyticus (VP) containing a large virulent plasmid (pVA1). Despite much research on AHPND, molecular pathogenesis of AHPND and host responses in AHPND-infected shrimp are not well characterized. Therefore, the objective was to use transcriptome sequencing, an NGS platform and bioinformatics to identify the key factor or pathway in the stomach of AHPND-infected shrimp. First, by detecting virulent gene Pir as a pathogen marker, we monitored shrimp infected with AHPND. Subsequently, samples were subjected to NGS to obtain a transcriptome database to assess host responses after AHPND infection. Using a TSB group (treated only with medium) as a control, we compared treatment groups to detect differential expression of genes (DEGs). Furthermore, a gene co-expression network between non-virulent and virulent VP-challenged groups was established using a ContigViews platform. From these networks, we proposed that AHPND infection affected immune responses in the stomach. We anticipate that this network will be a critical tool to understand the association between V.parahaemolyticus and shrimp, and elucidate key factors of AHPND infection.

林家弘，探討蝦類Dscam於白點症病毒感染時之免疫機制，國立成功大學生物科技研究所碩士論文，2014。

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