HIV-1病毒是一種反轉錄病毒，其病毒RNA上的引子結合區(PBS)會與tRNALys3之反引子結合區(anti-PBS)進行互補交互作用，產生18組鹼基對，並開啟一連串反轉錄步驟。本文乃以分子動力學研究觀察此一作用區域之動態行為、分子穩定性及結構特徵，結果顯示在胺基酸接受端(3’-CCA)與反密碼環為整個tRNALys3中較不穩定的部分，而在其他種類之tRNA亦有類似之結果。此外，本文並比較不同長度之PBS與tRNALys3交互作用後的差異性，藉此了解TΨC臂下半部的PAS對反轉錄作用所產生之影響。由本文的研究結果發現，發現隨著anti-PAS上被占住的氫鍵數的增加，活性則隨著降低，反之則增加。

Virus HIV-1 is one of retrovirus, which RNA primer binding of virus (PBS) will complementary interact with anti-primer binding site(anti-PBS) in tRNALys3. There are 18 base pairs and a series of reverse transcription steps will be produced during the complementary interaction of PBS and anti-PBS. In this study, molecular dynamics is utilized to observe the dynamic behavior , molecular stability and the structure of tRNALys3. The results show that in the amino acid accept stem (3’-CCA) and the anti-codon loop are the unstable part in tRNALys3. The similar results are found in the other type of tRNA. Additionally in order to realize the efficiency of reverse transcription of primer activation signal (PAS), the difference between the interaction of primer binding site (PBS) and tRNALys3 with different lengths is also compared in this study. The experimental results, In this study, the results shows that the activity will decrease with the increasing of the hydrogen bonds in anti-PAS.

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