本研究以改良的Hummer法來製備氧化石墨烯(Graphene Oxide，GO)，並以十八烷胺(octadecylamine， ODA) Langmuir單分子層作為模板，藉由單分子層與GO及葡萄糖氧化酵素(Glucose Oxidase, GOx)的作用力，將水溶液中的GO及GOx吸附至氣/液界面，以製備GO及GOx的單分子膜及Langmuir-Blogett (LB)膜。由單分子層的表面壓-時間吸附曲線(π-t isotherm)及表面壓-分子佔據面積等溫曲線(π-A isotherm)來探討GO/ODA及GOx/ODA混合單分子膜於氣/液界面的行為。並以電子顯微鏡和原子力顯微鏡來觀察LB薄膜的表面構型。接著以紫外光還原含氧官能基形成還原態氧化石墨烯(Reduced Graphene Oxide, rGO)薄膜，之後分別將GO及rGO沉積至白金基板，製備成生物感測器進行電化學分析並探討其對感測效能的影響。由實驗結果發現，藉由ODA吸附溶液中的GO可於氣/液界面上形成GO單分子膜，且其表面覆蓋率及表面型態可藉由表面壓的大小來控制。由電化學實驗發現，經紫外光還原過的rGO，有較佳的電化學活性，且在基板上沉積3層rGO時，對過氧化氫有最佳的感測能力，而其葡萄糖感測器靈敏度可達到5.808 μA/cm2∙mM。

In order to prepare graphene oxide (GO) and glucose oxidase (GOx) monolayer, we functionalize the graphite with oxidize agent by modified Hummer’s method. Moreover octadecylamine (ODA) monolayer was used as a template to adsorb GO and GOx from solution to air/water interface by interactions between the template and GO or GOx. We study the behavior of the GO/ODA and GOx/ODA mixed monolayer at air/water interface by pressure-time (π-t) isotherm and the pressure-area (π-A isotherm) isotherm. The morphology of the GO/ODA LB film was examined using Transmission electron microscope (TEM) and Atomic force microscope (AFM). In order to regain the film conductivity, we expose the GO/ODA LB film under UV light to form Reduced Graphene Oxide (rGO). Then the GO and rGO monolayer were dipped on the platinum for biosensors fabrication to study electrochemical property and to get the sensor sensibility. According to experiment results, GO can be adsorbed by surfactant on air/water interface and form GO monolayer. The surface coverage and morphology of the monolayer on the air/water interface can be controlled by surface pressure. From the electrochemical experiment, rGO which reduced by UV light has better electrochemical activity. It shows the best sensing ability to hydrogen peroxide when dipping 3 layers of rGO on the substrate. Moreover the glucose sensor exhibits the highest sensitivity(5.808 μA/cm2∙mM).

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