隨著工業革命的興起大氣中的二氧化碳濃度上升，造成溫室效應加劇全球氣候變遷。各界開始找尋各種碳捕獲與封存(CCS)技術以降低大氣中的碳含量。其中利用生物固碳法被視為具有潛力發展及永續性。
藍綠菌在生長期間可捕捉二氧化碳並同時生產具有保健功用的玉米黃素、β胡蘿蔔素。玉米黃素、β胡蘿蔔素為藍色捕光色素，具有抗氧化能力，因此被認定為有助於健康的成分可應用於保健食品當中。玉米黃素、β胡蘿蔔素為視網膜主要色素，玉米黃素主要存在於黃斑部分，攝取玉米黃素及β胡蘿蔔素可預防白內障、老年性黃斑部病變(AMD)等眼部病變。
光強度、氮源濃度及鹽度可能是影響藍綠菌玉米黃素、β胡蘿蔔素累積的關鍵因素。在本研究中，利用嗜熱藍綠菌Thermosynechococcus sp. CL-1 （TCL-1）以評估在不同光照強度（500、1,000、1,500 μE m-2 s-1），氮源濃度（1.2-29.2mM）與鹽度（0.14, 0.3, 0.5 M）培養下，其生質體產率、二氧化碳固定速率和玉米黃素、β胡蘿蔔素產率的影響。
研究結果顯示，最大生質體產率發生在光照強度為1,000 μE/m2/s和5.8 mM初始氮源濃度下為90.3 ± 3.9 mg/L/h。最大固碳速率為129.1 ± 5.5 mg/L/h，最大玉米黃素產率為0.074 mg/L/h、β胡蘿蔔素產率為0.39 mg/L/h。
由本研究之結果可知，固碳速率與玉米黃素、β胡蘿蔔素產率的最大影響因素皆在於生質體產率，其次才為細胞中碳含量與玉米黃素、β胡蘿蔔素含量。因此將本研究結果比較後，發現在以光照強度為1,000 μE/m2/s，氮源濃度為5.8 mM且鹽度為0.14 M培養下，有較優勢的生質體產率、固碳速率與玉米黃素、β胡蘿蔔素產率。

Since industrial revolution, CO2 concentration in atmosphere has risen sharply. Excess green house gas (GHG) has influences on extreme weather and global warming. Carbon capture and sequestration (CCS) technologies are therefore under researched in recent decades to solve the problems. Biological carbon mitigation (BCM) was considered as a sustainable and potential process which uses autotrophic organisms such as microalgae and cyanobacteria to absorb CO2 from atmosphere through photosynthesis.
In this study, the combination of the advantage of the chemical-alkaline-absorption and BCM is applied as the technology of carbon fixation from one of the main CO2 emission sources, power plant. CO2 has much more solubility in the alkaline solution and becomes HCO3- or CO32- as the carbon source for cyanobacteria. For meeting this practical requirement, thermophilic and basophilic cyanobacteria Thermosynechococcus sp. CL-1 (TCL-1) was chosen in this study. In order to increase the biomass productivity and carbon fixation rate, the higher surface-area-ratio flat panel was used as the photobioreactor (PBR) with high initial biomass concentration. Light intensity, DIN concentration and salinity have been needed as the operating parameters to affect the accumulation of the zeaxanthin and β-carotene in several researches.
The results show TCL-1 can achieve the highest biomass productivity 90.3 mg/L/h, carbon fixation rate 129.1 ± 5.5 mg/L/h, biomass increment 38.0 ± 2.5%, the maximum zeaxanthin content 0.325 ± 0.009 mg/g at 8h, the maximum β-carotene content 1.92 ± 0.02 mg/g at 12h, the maximum zeaxanthin productivity 0.074 ± 0.004 mg/L/h at 8h, and the maximum β-carotene productivity 0.39 ± 0.03 mg/L/h at 12h if cultivated in the 5.8 mM initial DIN under 1,000 μE/m2/s with 0.14 M salinity.

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