本實驗欲設計合成以十二到十四烷基縮水甘油醚製備疏水修飾聚乙烯胺。首先將N-乙烯甲醯胺單體(N-vinylformamide)利用傳統自由基聚合法(free radical polymerization) 聚合成聚乙烯甲醯胺高分子，再加入氫氧化鉀使聚乙烯甲醯胺高分子水解為聚乙烯胺。將聚乙烯胺在甲醇或甲醇/水共溶劑為反應溶劑，並調整不同進料莫爾比例得到不同取代度（疏水基占整體的比例）疏水修飾聚乙烯胺。再將疏水修飾聚乙烯胺做螢光光譜分析，以及粒徑分析。
在相同取代度下，疏水修飾聚乙烯胺濃度越高，由螢光光譜得到的[I1/ I3]py越低，代表溶液疏水性越高。同時，由動態光散射得到得粒徑結果顯示粒徑越來越小，說明疏水修飾聚乙烯胺因為疏水性越來越大，而使得高分子鏈段疏水區域逐漸聚集，導致粒徑隨濃度變高而降低。當疏水修飾聚乙烯胺濃度不變，pH值越高時，得到的[I1/ I3]py越低，代表溶液疏水性越高。粒徑結果越低，顯示疏水修飾聚乙烯胺逐漸由舒展的直鏈狀高分子，轉變為球狀高分子。
當取代度越高時，在相同濃度下 [I1/ I3]py越來越小，反映溶液的疏水性越高，證實疏水修飾聚乙烯胺的疏水基比例越大。當取代度越高時，在相同濃度下粒徑越來越小，也反映溶液的疏水性越高，高分子鏈段疏水區域逐漸聚集致使粒徑逐漸下降。在取代度越高的疏水修飾聚乙烯胺，粒徑隨濃度增加而下降的速率越快。在疏水修飾聚乙烯胺的取代度變大時，[I1/ I3]py下降速率和粒徑下降速率漸增。代表濃度造成疏水性和粒徑影響越大。

Hydrophobically modified poly(vinylamine) was successfully synthesis by ring-opening reaction of epoxides from dodecyl to tetradecyl glycidyl ether and poly(vinylamine). Poly(vinylamine) were hydrolyzed from poly(N-vinylformamide) with 1 M Potassium hydroxide solution at 70˚C for 24 hours, and purification of Poly(vinylamine) were by dialysis. Poly(N-vinylformamide) were synthesized via free radical polymerization of N-vinylformamide monomer with AIBN in toluene at 65˚C for 12 hours. , and purification of Poly(N-vinylformamide) were by reprecipitation using acetone. The ratio of hydrophobic and hydrophilic group of hydrophobically modified poly(vinylamine), in other words, degree of substitution, which means the percentage of hydrophobic groups to entire hydrophobically modified poly(vinylamine), was determined by 1H NMR.

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