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研究生：	王珮馨 Wang, Pei-Hsin
論文名稱：	以新穎亞硝酸鹽/硝酸鹽型厭氧甲烷氧化菌處理含硝酸氮高導電度工業廢水之評估 Evaluation of Nitrite/Nitrate Dependent Anaerobic Methane Oxidation (N-DAMO) Microorganisms for nitrate removal in high conductivity industrial wastewater
指導教授：	黃良銘 Whang, Liang-Ming
學位類別：	碩士 Master
系所名稱：	工學院 - 環境工程學系 Department of Environmental Engineering
論文出版年：	2020
畢業學年度：	108
語文別：	英文
論文頁數：	110
中文關鍵詞：	厭氧流體化薄膜反應槽、工業廢水、導電度、初始蛋白質濃度
外文關鍵詞：	AFMBR, industrial wastewater, conductivity, initial protein concentration
相關次數：	點閱：77 下載：0
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亞硝酸鹽/硝酸鹽型厭氧甲烷氧化菌群利用甲烷作電子提供者及碳源進行脫硝。污水處理廠的厭氧處理流程產生甲烷，回收利用後可避免甲烷逸散至廠外，亦可作亞硝酸鹽/硝酸鹽型厭氧甲烷氧化菌群脫硝之碳源，是近來越多研究關於應用亞硝酸鹽/硝酸鹽型厭氧甲烷氧化菌群於污水處理廠的原因。
本研究以厭氧流體化薄膜反應槽培養亞硝酸鹽/硝酸鹽型厭氧甲烷氧化菌群，以稀釋過的工業廢水為進流，工業廢水導電度為14.5 mS/cm，含濃度90.1 mg-C/L有機碳、159.4 mg-N/L硝酸氮。反應槽進流硝酸氮濃度為45.6 mg-N/L，水力停留時間1.6天時，取汙泥進行脫硝批次實驗並以多元線性迴歸分析。硝酸氮濃度範圍介於43至63 mg-N/L，硝酸氮、有機碳、溫度和甲烷去除量對總硝酸氮去除率有正面影響，其係數分別為0.2、0.1、4.2及0.9，反之，初始蛋白質濃度對總硝酸氮去除率有負面影響，其係數為-0.5。
與不提供甲烷的組別相比，廢水中有機碳降低亞硝酸鹽/硝酸鹽型厭氧甲烷氧化菌群之活性。移除廢水中有機碳，在硝酸氮濃度45 mg-N/L、導電度5.0 mS/cm及甲烷濃度0.78 mmol/L時，其亞硝酸鹽/硝酸鹽型厭氧甲烷氧化菌群之貢獻可達82%，培養亞硝酸鹽/硝酸鹽型厭氧甲烷氧化菌群時，應納入考量。

N-DAMO microorganisms utilize methane as electron donor and carbon source for denitrification. For the reason that methane is produced during anaerobic process in wastewater treatment plant, reusing methane can simultaneously solve problem of methane emission and deficiency of carbon in wastewater for denitrification. Therefore, application of N-DAMO microorganisms in wastewater treatment process has been popular in recent years.
An industrial wastewater, with conductivity of 14.5 mS/cm, 90.1 mg-C/L of TOC, and 159.4 mg-N/L of nitrate, was used to evaluate nitrate removal by N-DAMO microorganisms in this study. With low growth rate, N-DAMO microorganisms were enriched in an AFMBR system, and diluted industrial wastewater was applied as substrate. When influent nitrate concentration applied to AFMBR system was 45.6 mg-N/L and HRT was 1.6 days, sludge was taken out from AFMBR system for denitrification batch experiments, and multiple linear regression was used to analyze. With initial nitrate concentration ranging from 43 to 63 mg-N/L, initial nitrate concentration, TOC, methane concentration and temperature had positive effect on total nitrate removal with coefficient of 0.2, 0.1, 4.2, and 0.9, while initial protein concentration adversely affected total nitrate removal with coefficient of -0.5.
TOC in industrial wastewater decreased N-DAMO microorganisms’ activity comparing with batches without purging methane. When TOC was excluded from industrial wastewater, N-DAMO contribution could achieve 82% with high nitrate of 45 mg-N/L, high conductivity of 5.0 mS/cm, and high methane concentration of 0.78 mmol/L. As a result, these factors should be considered while enriching N-DAMO microorganisms.

摘要	I
Abstract	III
致謝	V
Table of Content	VII
List of Tables	XI
List of Figures	XIII
Chapter 1 Introduction	1
Chapter 2 Literature Review	3
2-1 Biological removal of nitrogen	3
2-2 Aerobic and anaerobic oxidation of methane	7
2-3 Nitrite/nitrate-dependent anaerobic methane oxidation   (N-DAMO) process	13
2-3-1 discovery of N-DAMO process	13
2-3-2 microorganisms in N-DAMO process	14
2-3-3 potential mechanisms of N-DAMO process	17
2-3-4 Reactors design for enriching N-DAMO microorganisms culture	20
2-3-5 Interaction between Anammox and N-DAMO microorganisms	24
2-3-6 N-DAMO process in wastewater treatment plant	26
2-4 Nitrogen removal in High conductivity wastewater	26
2-4-1 N-DAMO process in high salinity wastewater	27
2-5 Factors when cultivating N-DAMO microorganisms	28
2-6 Methane solubility	30
Chapter 3 Materials and Methods	33
3-1 Research framework	33
3-2 Characteristics of industrial wastewater	34
3-3 Enrichment strategy	36
3-3-1 Inoculum and batch test	36
3-3-2 Medium preparation	36
3-4 Anaerobic fluidized membrane bioreactor system	38
3-5 Batch experiment for denitrification in Reactor A without argon applied as control batches	43
3-6 Batch experiments under different environments with argon applied as control batches	48
3-7 Analytical Methods	53
3-7-1 Water quality analysis	53
3-7-2 Gas composition analysis	55
3-7-3 Protein analysis	56
3-8 Calculation	57
3-8-1 Reactor A and B	57
3-8-2 Batch experiment for denitrification in Reactor A without argon applied as control batches	59
3-8-3 Batch experiments under different environments with argon applied as control batches	63
Chapter 4 Results and Discussion	66
4-1 Reactor A	66
4-1-1 Inoculum batch test	67
4-1-2 Fed-batch in AFMBR	67
4-1-3 Continuous feeding in AFMBR	70
4-2 Reactor B	78
4-2-1 Inoculum and fed-batch in AFMBR	78
4-2-2 Continuous feeding in AFMBR	79
4-3 Protein concentration in Reactor A and B	82
4-4 Batch experiments for denitrification in Reactor A without argon applied as control batches	85
4-5 Batch experiments under different environments with argon applied as control batches	91
Chapter 5 Conclusions and Suggestions	101
5-1 Conclusions	101
5-2 Suggestions	102
Chapter 6 References	103
 

                                    

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陳瑩禎，利用亞硝酸鹽/硝酸鹽型厭氧甲烷氧化菌進行脫硝除氮之研究，國立成功大學環境工程學系碩士論文，2018年

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