石斑魚是亞洲具經濟價值的水產養魚種之一。然而，隨著石斑魚的成 長他們可能面對環境中的各種緊迫，像是感染神經壞死病毒、氣候變遷及 鹽濃度的變化。自噬作用被生物作為對抗緊迫環境的防禦策略，此機制為 重要細胞內負責降解及循環胞器和蛋白的途徑之一。而微管相關蛋白1 輕 鏈3（LC3）為是自噬體的組成成分之一，並被作為自噬作用的可靠標記 物。然而，對於自噬作用與緊迫的調節在點帶石斑魚中還是未知的。因此， 在本研究以LC3 作為標記物來探討石斑魚中被緊迫所誘發的自噬作用在 不同刺激物下的反應。osgLC3 全長為1440 鹼基對，可以被轉譯出126 個 胺基酸。從即時聚合酶鏈式反應分析，osgLC3 的表現在點帶石斑魚的每 個測試組織中具有高表現。此外，結果顯示osgLC3 對溫度變化、鹽度變 化和免疫刺激有反應。另外，在自然感染NNV 點帶石斑幼魚，在神經系 統腦和眼睛osgLC3 表現比控制組高約十倍。總結這些研究結果是利用 osgLC3 證明了自噬作用和緊迫環境間的相互作用。最後，這項研究說明 當石斑魚面臨環境壓力會誘導自噬作用，可能有助於生物適應環境變化， 藉由通過破壞受損的胞器和蛋白質或病毒蛋白來維持細胞平衡。

Groupers are one of the most remunerative aquaculture species in Asia. However, as groupers grow up, there are various stress conditions that they might face, such as nervous necrosis virus (NNV) infection, salinity changes and climate changes. Autophagy is used by organisms as a defense strategy against environmental stress. The mechanism is one of the most important intracellular pathways responsible for the degradation and recycling of organelles and proteins. Microtubule-associated protein light chain 3 (LC3) is one of the constituents of the autophagosome and utilized as a reliable marker for autophagy. However, the stress-regulating function of autophagy is still not yet known in orange-spotted groupers. In this study, investigating stress-induced autophagy by the LC3 marker in grouper in response to different stimulations. The full-length sequence of orange-spotted grouper LC3 (osgLC3) gene had been cloned with 1,440 bp that can be translated to 126 amino acid sequences. From the qPCR analysis, it showed that osgLC3 had high basal expression levels in every tested tissue from orange-spotted groupers. In addition, the results showed that osgLC3 were response to temperature changes, salinity change, and immune stimulations. Besides, in naturally NNV-infected orange-spotted grouper larvae showed that the high expression level of osgLC3 about 10-fold in nervous system-brain and eye compared with control group. Taken together, these studies demonstrated that the interaction between autophagy and stress condition confirmed by osgLC3. Eventually, this study suggested that when groupers faced environmental stress and induced autophagy may be helpful to adapt to environmental changes that keeps up cellular homeostasis by destructive of damaged organelles and proteins or viral proteins.

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