目前光電產業蓬勃發展，台灣也不例外，產生之廢水量也相當可觀，而光電廢水則屬於高氮廢水，且其成分相當複雜，大多不易被一般好氧微生物利用。因此，實廠則以結合傳統活性污泥與薄膜技術之生物薄膜反應器(MBR)來處理光電廢水，以達到廢水回收的目的。而在處理系統中，則必須注意其硝化效能，以提升整體除氮之效率。由於MBR中使用之UF薄膜會將微生物死亡後釋放的物質或本身產物如溶解性微生物產物(SMP)與胞外聚合物(EPS)截留在好氧槽內，而這些物質會造成好氧槽中COD濃度上升，即稱為膠體性COD，而過去觀察發現膠體性COD會對硝化造成影響，故本研究的目的則是對膠體性COD作進一步解析，並找出其與硝化之間更加確切的關係。
本研究對兩個不同廠區監測其水質，並且對其好氧槽之膠體性COD以高效能粒徑排除層析儀(HPSEC)與螢光激發/發散陣列光譜儀(FEEM)進行分析，其結果發現兩個廠區好氧槽中所含不同大小之分子量有機質會造成不同的硝化表現。主要測得有機質含碳碳雙鍵疏水性有機物、溶解性微生物副產物、類黃酸、類腐植酸與芳香烴蛋白，當分子量大於106 Da之物質主要就是膠體性COD的來源，而分子量大於107 Da之物質則明顯影響到硝化作用，而影響硝化較為明顯之物質是較大分子的芳香烴蛋白物質。
另外，以實驗室馴養的兩組不同好氧污泥進行硝化測試批次，以馴養207天之好氧污泥其比硝酸生成速率(S.NPR)與比氨氧化速率(S.AOR)分別為0.513與0.322 mg-N/g VSS-hr，而馴養87天(以F(B)上層液作為進流)好氧污泥其比硝酸生成速率(S.NPR)與比氨氧化速率(S.AOR)分別為1.046與0.749 mg-N/g VSS-hr，發現造成此硝化效能差異的原因是實驗進行中的18小時，硝化較差那一組中分子量大於107.48 Da之溶解性微生物產物與芳香烴蛋白類的物質突然上升的緣故。
膠體性COD成分中所含較大分子之有機物質會影響MBR處理光電廢水中之硝化效能，若能解決膠體性COD的問題，並搭配上適合硝化菌生長之操作參數，則能使實廠整體系統處理效能提升。

Due to the amount wastewater of thin film transistor liquid crystal display (TFT-LCD) that increased in Taiwan. The TFT-LCD wastewater was the nitrogen-rich and the composition of them was so complicated that the general microorganisms used difficultly. Therefore, the membrane bioreactor (MBR) combined the activated sludge process with the membrane technology was applied in the TFT-LCD wastewater treatment. In MBR, the soluble microbial products (SMP) and extracellular polymeric substances (EPS)that produced by microorganisms. The SMP and EPS were intercepted by ultrafiltration membrane. The substances called the colloidal COD that cause the COD concentration increased in the aerobic tank. Hence, the aim of this study is analyzed the composition of the colloidal COD and found the accurate relationship between the colloidal COD and nitrification.
The composition of the colloidal COD in two full-scale treatment plant was analyzed by the high performance size exclusion chromatography (HPSEC) and Excitation/Emission Matrix Spectrofluorometer (FEEM). The different molecular weight of organic materials were observed in two full-scale MBR with different nitrification performance. Higher portion of organic compounds were larger than 107 Da in the MBR with bad nitrification. The results show the higher molecular weight of aromatic protein was obvious one of organic compounds to effect nitrification.
Nitrification activity batch experiments were also conducted using sludge from lab-scale MBR systems. The result shows that SMP-like and AP-like materials which might be released from microorganisms could cause bad nitrification.
Thus, organic compounds, or colloidal COD which due probably to long-time operation might influence nitrification performance in membrane bioreactors (MBR) treating TFT-LCD wastewater.

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