本研究主要在瞭解以懸浮式連續流方式，採用甲苯/三氯乙烯之最佳濃度配比為20，培養甲苯分解菌（T1菌）共代謝三氯乙烯之生物降解情形，並探討固定水力停留時間，不同基質濃度對連續流試驗之影響，以及改變水力停留時間後，微生物降解甲苯及三氯乙烯之變化影響。
在水力停留時間三天的操作條件下，改變基質濃度對共代謝三氯乙烯之影響方面，添加不同三氯乙烯濃度（0.5、1和2 mg/L）及不同甲苯濃度（10、20和40 mg/L）操作下，在試驗開始後即能展現良好共代謝效果，三氯乙烯去除率皆能達到97.9 %以上。在三氯乙烯的出流濃度方面，各組之三氯乙烯最終出流濃度分別為3.8、16.0和59.0 μg/L，顯示基質濃度的提高對三氯乙烯之共代謝效率略微抑制。
改變水力停留時間之連續流試驗方面，在2天的水力停留時間操作下，各組之甲苯及三氯乙烯代謝效果依然良好，其三氯乙烯去除率能達到95.8 ％以上，但低水力停留時間下之三氯乙烯出流濃度較第一階段高水力停留時間連續流試驗之各組稍高，其出流濃度分別為8.1、38.0和75.4μg/L，顯示在較長的水力停留時間下，微生物能將三氯乙烯代謝的更完全。
試驗期間之pH和溶氧量並無太大之變化（pH：5.8～6.6、DO：1 mg/L以上），顯示懸浮式連續流甲苯共代謝三氯乙烯系統之操作良好。

The main objective of this study is to find the best operate conditions for cometabolize trichloroethylene (TCE) using Toluene in a continuous stirred tank reactor (CSTR). We cultivate that toluene degrading bacterium strain T1 with the best toluene/TCE (20/1) ratio to cometabolize TCE. Also, different HRT and substrate concentration are explore the influence of TCE cometabolism in the CSTR.
For the TCE cometabolism experiments, Toluene and TCE could be rapidly degraded in the initial stage. The TCE removal efficiency could reach upward 97.9% with the HRT of 3 days. When TCE concentration at 0.5, 1 and 2 mg/L, TCE was partially removed by the Toluene degraders to reach 3.8, 16.0 and 59.0 μg/L, respectively. The results show that high initial concentration of TCE would restrain the efficiency of TCE cometabolism using Toluene. On the other hand, the cometabolic TCE removal efficiency decreased with the initial concentration of TCE raised.
When HRT changed from 3 days to 2 days, TCE removal efficiency could still reach upward 95.8 %. When TCE concentration at 0.5, 1 and 2 mg/L, TCE was partially removed by the Toluene degraders to reach 8.1, 38.0 and 75.4 μg/L, respectively. The results show that lower HRT could not cometabolize TCE much completely than higher HRT.
In addition, dissolved oxygen and pH didn’t have evident variation (pH: 5.8~6.6 and dissolved oxygen: greater than 1 mg/L) during the experiment periods. It seems to be lower relation among pH, dissolved oxygen, and TCE removal efficiency in the above operation condition.

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