組織胺是生物胺的其中一種，攝入含有大於50 ppm組織胺的食品就可能對人體造成傷害，產生多種組織胺食品中毒症狀。由於人體感官可能無法察覺食品中的組織胺含量是否超過50 ppm，因此需要發展一個食品中組織胺含量檢測裝置，以避免此類食品中毒事件的發生。網版印刷電極成本便宜，具有量產的潛力，但其電化學活性較金屬電極差，對組織胺亦不具有催化性與專一性，因此需藉由表面修飾處理，使得其對組織胺的靈敏度與專一性提高。本研究使用具有良好電子傳導性與電化學活性的還原態氧化石墨烯進行網版印刷碳電極之修飾，以放大訊號和增加靈敏度。並搭配雙胺氧化酵素之修飾，使裝置對組織胺具有專一性。本裝置的量測線性範圍介於20~ 200 ppm (R2 > 0.99)，已有初步的成果，其線性範圍並涵蓋食品實際樣本檢測所需的範圍，未來會更進一步研究，使整體裝置更加穩定，以及減少實際樣本中干擾物之影響等，以求此裝置未來能夠實際應用於食品中組織胺含量之檢測。

Histamine is one of the biogenic amines that may cause food poisoning when intake food contains high concentration of histamine. The critical limitation of histamine in food is 50 ppm. Food containing histamine higher than 50 ppm may harm human body. Since human sensory organ cannot sense such concentration of histamine, it’s necessary to develop a device to detect histamine in food. Screen-printed carbon electrode (SPCE) is a kind of electrode that is cheap, easy to prepare, and has the potential to mass production and commercialization. But the electrochemical activity of SPCE is lower than other metal materials, and it has no selectivity and specificity to histamine. This study applied reduced graphene oxide (RGO) and diamine oxidase (DAO) to modify the surface of SPCE to detect samples containing different concentration of histamine. The linear range of this device is 20 to 200 ppm (R2 > 0.99) that meets the requirement of food real sample detection. The selectivity, stability, and recovery rate of the device should be improved before this device is applied to food quality control use.

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