隨著科技的進步，奈米材料的研究發展非常迅速，其中磁性奈米粒子已廣泛運用在生物醫學的領域上。由於磁性奈米粒子所具有的超順磁特性，可以利用外加磁場加以控制作為藥物標靶或藥物輸送的載體，藉由具靶向性的蛋白，精準的將藥物送至病灶所在的位置，以此控制病情或達到治療的效果，在生物醫學上相當具有研究價值。
本研究以水相共沉澱法 (co-precipitation) 製備磁性奈米粒子，並在其外以 3-氫硫丙基三甲氧基矽烷 (3-mercaptopropyltrimethoxysilane, MPTMS) 修飾，接著以 11-氫硫基十一烷酸 (11-mercaptoundecanoic acid, MUA) 做雙硫鍵的橋接與功能性修飾，使其外層帶有羧基，而後以運鐵蛋白 (Transferrin, Tf) 上的胺基與前者之羧基連結，作為一個具有靶向性的磁性奈米粒子載體。
以 X 光繞射儀 (XRD) 證實磁性奈米粒子為四氧化三鐵 (Fe3O4) 晶相，並推算Fe3O4, Fe3O4@MPTMA, Fe3O4@MPTMS@MUA 平均粒徑分別為 9.93, 10.18, 11.33 nm；以穿透式顯微鏡 (TEM) 在觀察粒子的表面形態後所估算的平均粒徑分別為 9.39 ± 1.47, 11.08 ± 1.38 及 13.07 ± 2.00 nm；以傅立葉轉換紅外線光譜儀 (FT-IR)、拉曼光譜儀 (Raman)、熱重分析儀 (TGA) 及能量散射光譜儀 (EDS) 確認修飾物質確實以化學鍵結包覆在磁性奈米粒子外層；以超導量子干涉儀 (SQUID) 所得之磁滯曲線得知，Fe3O4, Fe3O4@MPTMA, Fe3O4@MPTMS@MUA 的飽和磁化量分別為 83.30, 73.66 及 61.26 emu/g，且皆具有超順磁的性質，顯示包覆物會影響其飽和磁化量，但不影響其磁性質。
在生物醫療應用上，以運鐵蛋白進行連接，以紫外光/可見光光譜儀 (UV/Vis) 判斷運鐵蛋白是否連結於磁性粒子表面上，經估算後每毫克 Fe3O4@MPTMS@MUA 粒子可包覆 0.848 毫克運鐵蛋白，未來可用於攜帶藥物至有運鐵蛋白受體之細胞上。

With the improvement of technology, magnetic nanoparticles were applied in bio-medical field extensively. For the superparamagnetism it has, magnetic nanoparticles can be used as the carrier of drug targeting or drug delivery, controlled by external magnetic fields through targeted protein, and sends the medicine to the lesion accurately. Hence the illness will be controlled and the effect of the treatment will be achieved, which is worth of researching.
In this research, the magnetic nanoparticles were synthesized by co-precipitation in water phase, modified by 3-mercaptopropyltrimethoxysilane. And then modified functionally and bridged disulfide bond with 11-mercaptoundecanoic acid to cover the surface with carboxyl group. After that, the carboxyl group of the former was tied with the amino group of transferrin. Finally, a kind of magnetic nanoparticle carriers for targeting was made.
We used XRD to confirm Fe3O4 structure of magnetic nanoparticles. The average diameter of Fe3O4, Fe3O4@MPTMA, Fe3O4@MPTMS@MUA were estimated to be 9.39 ± 1.47, 11.08 ± 1.38 and 13.07 ± 2.00 nm. We confirmed that the surface of magnetic nanoparticles was coated with the modifications in the form of chemical bonds, through FT-IR, Raman, TGA and EDS. SQUID results indicated that the saturation magnetization of Fe3O4, Fe3O4@MPTMA, Fe3O4@MPTMS@MUA were 83.30, 73.66 and 61.26 emu/g respectively, and with superparamagnetism on them. And it showed that coating would change the magnetization without changing its magnetic properties.
For bio-medical application, whether the transferrin was tied on the surface of magnetic nanoparticles was detected by UV/Vis. It was estimated that Fe3O4@MPTMS@MUA each milligram can be coated with 0.848 mg transferrin, which was expected to be used to carry medicine to cells with the receptor of transferrin.

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