本研究分別以粒子觀測法及電流觀測法量測微管道中非均質壁面的zeta potentials。粒子觀測法是在微管道內注入添加螢光粒子的電解質溶液，並在管道兩端裝置電極，然後施加電壓產生電場，以引發電滲流，及螢光粒子的電泳運動，藉著粒子速度分佈的測量及理論模式的最佳化分析求得壁面的zeta potential。電流觀測法則是使微管道兩端分別連接不同濃度電解質溶液的儲槽，在外加電場下，所引發的電滲流會造成微管道內的電解質溶液取代現象，使得量測的電流值發生變化，而進一步求得壁面的zeta potential。實驗結果顯示使用粒子觀測法並無法得到預期的zeta potential結果。但在電流觀測法方面，本研究提出的理論模式不僅可提高微管道中均質壁面zeta potential量測的準確性，亦可應用在非均質微管道壁面zeta potential的量測上。

In this study, particle tracking method and current monitoring method are used to evaluate the zeta potentials of microchannel walls with different surface charges. For the particle tracking method, an electrolyte solution containing fluorescent particles was added into a microchannel with electrodes at two ends. A potential difference was then applied to introduce an electric field in the microchannel, resulting in electroosmotic flow and particle electrophoretic motion. From the measurement of fluorescent particle velocity distribution and the best-fit analysis with theoretical models, the zeta potentials of the microchannel walls can be evaluated. For the current monitoring method, two ends of a microchannel were connected to two reservoirs containing electrolyte solutions with different concentrations, respectively. By applying an electric field, the electrolyte solution displacement phenomenon was resulted by the induced electroosmotic flow, causing the variations in the measured current. The experimental results indicated that the particle tracking method was unable to obtain the expected zeta potential data. However, by using the current monitoring method, the theoretical models proposed in the study could be used to improve the accuracy in evaluating the zeta potentials of microchannel walls with a uniform surface charge. Moreover, the approach could be also applied to evaluate the zeta potentials for microchannel walls with different surface charges.

Bierbaum, K., Grunze, M., Baski, A.A., Chi, L.F., Schrepp, W., and Fuchs, H., “Growth of Self-Assembled n-Alkyltrichlorosilane Films on Si(100) Investigated by Atomic Force Microscopy,” Langmuir 11, 2143-2150, 1995.

Britt, D.W., and Hlady, V., “An AFM Study of the Effects of Silanization Temperature, Hydration, and Annealing on Nucleation and Aggregation of Condensed OTS Domains on Mica,” J. Colloid Interface Sci. 178, 775-784, 1996.

Brzoska, J.B., Shahidzadeh, N., and Rondelez, F., “Evidence of a Transition-Temperature for the Optimum Deposition of Grafted Monolayer Coatings,” Nature 360, 719-721, 1992.

Connah, M.T., Kaszuba, M., and Morfesis, A., “High Resolution Zeta Potential Measurement: Analysis of Multi-component Mixtures,” J. Dispersion Sci. and Tech. 23, 663-669, 2002.

Devasenathipathy, S., and Santiago, J.G., “Particle Tracking Techniques for Electronkinetic Microchannel Flows,” Anal. Chem. 74, 3704-3713, 2002.

Duffy, D.C., McDonald, J.C., Schueller, O.J.A., and Whitesides, G.M., “Rapid Prototyping of Microfluidic Systems in Poly(dimethylsiloxane),” Anal. Chem. 70, 4974-4984, 1998.

Flinn, D.H., Guzonas, D.A., and Yoon, R.H., “Characterization of Silica Surfaces Hydrophobized by Octadecyltrichlorosilane,” Colloids Surf. A: Physicochem. Eng. Aspects 87, 163-176, 1994.

Gas, B., Stedrý, M., and Kenndler, E., “ Peak Broadening in Capillary Zone Electrophoresis,” Electrophoresis 18, 2123-2133, 1997.

Huang, X., Gordon, M.J., and Zare, R.N., “ Current-Monitoring Method for Measuring the Electroosmotic Flow Rate in Capillary Zone Electrophoresis,” Anal. Chem. 60, 1837-1838, 1988.

Kirby, B.J. and Hasselbrink, Jr. E.F., “Zeta Potential of Microfluidic Substrates: 1. Theory, Experimental Techniques, and Effects on Seprations,” Electrophoresis 25, 187-202, 2004a.

Kirby, B.J. and Hasselbrink, Jr. E.F., “Zeta Potential of Microfluidic Substrates: 2. Data for Polymers,” Electrophoresis 25, 203-213, 2004b.

Le Grange, J.D., Markhan, J.L., and Kurkjian, C.R., “Effects of Surface Hydration on the Deposition of Silane Monolayers on Silica,” Langmuir 9, 1749-1753, 1993.

Locascio, L.E., Perso, C.E., and Lee, C.S., “ Measurement of Electroosmotic Flow in Plastic Imprinted Microfluid Devices and Effect of Protein Adsorption on Flow Rate,” J. Chromatogr. A 857, 275-284, 1999.

Mahabadi, K.A., Rodriguez, I., Haur, S.C., Kan, J.A., Bettiol, A.A., and Watt, F., “Fabrication of PMMA Micro- and Nanofluidic Channels by Proton Beam Writing: Electrokinetic and Morphological Characterization,” J. Micromech. Microeng. 16, 1170-1180, 2006.

Makamba, H., Kim, J.H., Lim, K., Park, N., and Hahn, J.H., “Surface Modification of Poly(dimethylsiloxane) Microchannels,” Electrophoresis 24, 3607-3619, 2003.

McGovern, M.E., Kallury, K.M.R., and Thompson, M., “Role of Solvent on the Silanization of Glass with Octadecyltrichlorosilane,” Langmuir 10, 3607-3614, 1994.

Minor, M., van der Linde, A.J., van Leeuwen, H.P., and Lyklema, J., “Dynamic Aspects of Electrophoresis and Electroosmosis: A New Fast Method for Measuring Particle Mobilities,” J. Colloid Interface Sci. 189, 370-375, 1997.

Probstein, R., Physicochemical Hydrodynamics, John Wiley & Son, New York, 1994.

Ren, L., Escobedo-Canseco, C., and Li, D., “A New Method of Evaluating the Average Electro-Osmotic Velocity in Microchannels,” J. Colloid Interface Sci. 250, 238-242, 2002.

Ren, L., Masliyah, J., and Li, D., “Experimental and Theoretical Study of the Displacement Process between Two Electrolyte Solutions in a Microchannel,” J. Colloid Interface Sci. 257, 85-92, 2003.
Sanders, R.S., Chow, R.S., and Masliyah, J.H., “Deposition of Bitumen and Asphaltene-Stabilized Emulsions in an Impinging Jet Cell,” J. Colloid Interface Sci. 174, 230-245, 1995.

Silberzan, P., Leger, L., Ausserre, D., and Benattar, J.J., “Silanation of Silica Surface. A New Method of Constructing Pure or Mixed Monolayers,” Langmuir 7, 1647-1651, 1991.

Sze, A., Erickson, D., Ren, L., and Li, D., “ Zeta-Potential Measurement Using the Smoluchowski Equation and the Slope of the Current-Time Relationship in Electroosmotic Flow,” J. Colloid Interface Sci. 261, 402-410, 2003.

Ulman, A., An Introduction to Ultrathin Organic Films, Academic Press, New York, 1991.

Wang, B., Abdulali-Kanji, Z., Dodwell, E., Horton, J.H., and Oleschuk, R.D., “Surface Characterization Using Chemical Force Microscopy and the Flow Performance of Modified Polydimethylsiloxane for Microfluidic Device Applications,” Electrophoresis 24, 1442-1450, 2003.

Wasserman, S.R., Tao, Y.T., and Whitesides, G.M., “Structure and Reactivity of Alkylsiloxane Monolayers Formed by Reaction of Alkyltrichlorosilane on Silica Substrates,” Langmuir 5, 1074-1087, 1989.

Werner, C., and Jacobash, H.J., “Surface Characterization of Polymers for Medical Devices,” Int. J. Artificial Organs 22, 160-176, 1999.

Yan, D., Yang, C., Nguyen, N.T., and Huang, X., “A Method for Simultaneously Determining the Zeta Potentials of the Channel Surface and the Tracer Particles Using Microparticle Image Velocimetry Technique,” Electrophoresis 27, 620-627, 2006.

葉宗儒，具圓形凹槽結構的微流道系統之流動特性探討及其在微混合器之應用，成功大學化學工程學系碩士論文，2005。