在皮膚傷口癒合的研究中，大致可分為細胞體外試驗和動物體內試驗兩大類，其中又以動物試驗較能模擬人類傷口癒合之狀況，因此，建立理想且穩定的動物傷口模式更是不可或缺的部分。在本研究中，以建立蘭嶼豬多重傷口開創之動物模式為目的，希望以此多重傷口作為陣列模式，提供簡易且快速地藥物篩選平台。試驗設計以證實蘭嶼豬動物模式之可行性，以及規劃具有快速且高通量篩選特性之傷口陣列為主，結合各種癒合參數進行評估。試驗結果顯示，以手術刀與電動取皮機開創不同深度之傷口分別可控制傷口大小在7.5%以及16.1%之誤差之內，在位置效應上，不同陣列位置對組織學結構以及傷口癒合與收縮之作用皆無顯著差異(p>0.05)。結合癒合參數之評估，部分深度傷口與全深度傷口在第7和9天傷口癒合上有顯著差異(p<0.005)且在第13天傷口收縮上有極顯著差異(p<0.001)。另外，也應用雷射都卜勒血流影像儀分析不同深度之傷口血流訊號變化趨勢。由實驗結果顯示，傷口陣列模式具有高度穩定性且不受陣列位置效應所影響，並且可結合各項參數進行評估，藉此也證實蘭嶼豬背側皮膚作為多重傷口的陣列模式之可行性。

Wound repair is a complex biologic process which is influenced by many factors and compounds. Both in vitro and in vivo models are widely-used in wound healing research. The ideal animal model should reflect human wound healing problems, so it is very important for us to establish the stable and consistent animal models for wound healing studies.
The purpose of this study is to establish the wound array model in Lanyu pigs, and use the wound array model as screening platform for drug therapy. In wound creating, the scalpel and dermatome are used to create full-thickness wounds and partial-thickness wounds. The result showed that wound size standard deviation of each creating method is 100%±7.5% and 100%±16.1%. And there is no significant site effect in pig dorsal skin by histological feature, wound closure and wound contraction parameters (p>0.05). Then, we use these wound healing parameters to analyze the difference between partial- and full- thickness wounds. There were significant differences in wound closure in day 7 and day 9 (p<0.005) and wound contraction in day 13 (p<0.001). In addition, we also use Laser Doppler Imager to analyze the blood flow signal during wound healing. Therefore, we suggest that the wound array model in Lanyu pigs which can combine many parameters to estimate for healing quality is a good platform for screening drug therapy.

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