人類毛髮在外觀上扮演著極重要的角色。毛髮生長起始自毛囊發育，毛髮成熟後會進入周而復始的毛髮週期。其中，真皮細胞和表皮細胞之間的交互作用扮演毛髮生成之重要角色。在此發育過程中有許多不同的生長因子參與其中。過去實驗室探究HR1於毛髮再生的影響。於是，本研究欲進一步使用一個可以運用在毛囊再生實驗上的細胞模式，並以此作為篩選平台，探討可幫助毛髮生成中，細胞層次的影響。在這邊，本實驗將以細胞增生、移行做為細胞模式篩選平台，篩選出促毛髮生成之HR因子與適當濃度，再將有效的HR因子分別以100 ng/ml濃度方式組合成為雞尾酒混合組，觀察兩組配對、三組配對與四組配對是否有促進增生或移行的效果。真皮細胞實驗結果顯示，HR1、3、4可促進真皮細胞增生，且HR3有最顯著之效果，HR1、4可促進真皮細胞移行，且HR4有最顯著之效果。表皮細胞HaCaT實驗結果顯示，HR1、3、4、8可促進表皮細胞增生與移行，但於表皮細胞增生中均無較突出之組別，不過在表皮細胞移行中，凡是涉及HR3者均有較為顯著的效果。綜合上述，以此細胞模式篩選平台篩選對真皮、表皮細胞增生、移行有效之毛髮生成相關因子、濃度與混合組合可做為未來幫助毛髮生長研究。

During the beginning of hair development, hair follicles develop to hair fibers and go to hair cycle. Hair formation involved multiple factors and epidermal-dermal cell interaction. In our pilot study a member found HR(Hair Regeneration)1 can enhance hair formation, so here we used the in vitro platform on hair formation to screen other optimal HR factors at cell level. In our study, we added HR factors, which were some mitogens for hair formation, to test how they affected hair formation. We performed proliferation and migration assays for screening and found optimal concentrations for HR factors. After single HR factor screening, we use the concentration of 100 ng/ml for each HR factor, later combine different factors together to designate as the cocktail treatment groups, for the optimization of HR effect. In our results, we found HR1, 3, and 4 enhanced dermal cell proliferation and HR3 has the best effect. In the migration assay, both HR1 and HR4 enhanced dermal cell migration and HR4 had the best effect. Using HaCaT as the cell model for epidermal cell, we found HR1, 3, 4, and 8 had positive effect on proliferation, and HR1, 3, 4, and 8 also have positive effect on migration. In cocktail treatments, we found that several groups involved HR4 can enhance dermal cell migration. The groups involved HR3 can enhance HaCaT migration. According to this study, in vitro screening assay can be a good platform for searching benefit factor on hair formation, and HR factors can enhance proliferation or migration of dermal and epidermal cells under different situations. Last but not least, we can use this cell platform to screen the optimal hair formation-related factors and their optimal concentration beneficial for hair formation.

陳彥蓉，Wnt10b 及β-catenin 在毛囊發育的表現，國立成功大學生物科技研究所碩士論文，2014。

梁志豪，HR1因子對於毛囊再生的作用，國立成功大學生物科技研究所碩士論文，2016。

黃珮青，血小板生長因子之功能性分析，國立成功大學生物科技研究所碩士論文，2011。

蔡淯亘，Wnt蛋白質在小鼠毛髮不同發育階段的分布，國立成功大學生物科技研究所碩士論文，2012。

羅韻綺，以體外皮膚培養系統研究微環境對於毛囊之初始發育，國立成功大學生物科技研究所碩士論文，2009。

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