本研究利用液胞粒徑、物理穩定性及界面電位的測量，探討幾丁聚醣（chitosan）披覆於帶負電之陰陽離子液胞表面的行為，以及披覆幾丁聚醣之液胞與DNA的結合行為。首先於水相中混合陽離子型界面活性劑hexadecyltrimethylammonium bromide（HTMAB）與陰離子型界面活性劑sodium dodecylsulfate （SDS），以得到離子對雙親分子（ion pair amphiphile ）hexadecyltrimethylammonium-dodecylsulfate（HTMA-DS）。接著將HTMA-DS、dihexadecyl phosphate（DHDP）及cholesterol以適當比例混合，利用強制形成的方法製備出總濃度4 mM的帶負電陰陽離子液胞分散液。再利用蠕動幫浦將4 mM的液胞分散液逐滴加到等體積的幾丁聚醣溶液中，以製備披覆幾丁聚醣之液胞的分散液。粒徑及界面電位的分析顯示，帶正電之幾丁聚醣藉由靜電吸引作用披覆於陰陽離子液胞的表面，且當混合系統中幾丁聚醣濃度達600~800 ppm時，披覆幾丁聚醣之液胞的初始粒徑趨於定值，表示幾丁聚醣在液胞上的吸附已達飽和。若陰陽離子液胞中含有較高莫耳分率的DHDP，則披覆幾丁聚醣之液胞具有較佳的物理穩定性，此現象可能與液胞表面的電荷分布及液胞雙層膜的穩定性有關。此外，幾丁聚醣濃度為800 ppm之液胞分散液，分別與等體積的125及250 ppm DNA溶液混合後，可形成帶正電且有良好物理穩定性的液胞/DNA複合物。凝膠電泳的結果也顯示，披覆幾丁聚醣之液胞與DNA有良好的結合。

This study investigated the behavior of chitosan coating onto the surface of negatively　charged catanionic vesicles and the association behavior of chitosan-coated vesicle with DNA from the measurements of vesicle size, physical stability, and zeta potential. An ion pair amphiphile (IPA), hexadecyltrimethylammonium-dodecylsulfate (HTMA-DS), was obtained first by mixing the cationic surfactant, hexadecyltrimethylammonium bromide (HTMAB), and anionic surfactant, sodium dodecylsulfate (SDS) in an aqueous phase. 4 mM negatively charged catanionic vesicle dispersions were then prepared with a forced formation method by mixing HTMA-DS, dihexadecyl phosphate (DHDP), and cholesterol in appropriate ratios. A chitosan-coated vesicle dispersion was made by adding the 4 mM catanionic vesicle dispersion dropwise into a chitosan solution with a 1:1 volume ratio by a tubing pump. Size and zeta potential analyses indicated that cationic chitosan coated onto surface of the catanionic vesicles by electrostatic attraction. While the chitosan concentration was about 600~800 ppm, the initial sizes of the chitosan-coated vesicles became constant, suggesting the adsorption of chitosan onto the vesicles was saturated. If the catanionic vesicles contained higher molar fractions of DHDP, the chitosan-coated vesicles possessed better physical stability, which might be related to the charge distribution of the vesicle surface and the stability of the vesicle bilayers. Furthermore, the vesicle dispersion containing 800 ppm chitosan mixed with 125 and 250 ppm DNA solutions, respectively, with an equal volume ratio could form vesicle/DNA complexes with positive charge characteristic and good physical stability. The results of gel electrophoresis also indicated that chitosan-coated vesicles could be well associated with DNA.

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