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研究生: 林芳妤
Lin, Fang-Yu
論文名稱: 黃韌帶肥厚背側與腹側在血管新生與纖維化訊號表現上的區域性差異
Regional Differences in Angiogenesis and Fibrotic Signaling Between Dorsal and Ventral Ligamentum Flavum Hypertrophy
指導教授: 涂庭源
Tu, Ting-Yuan
林政立
Lin, Cheng-Li
學位類別: 碩士
Master
系所名稱: 工學院 - 生物醫學工程學系
Department of BioMedical Engineering
論文出版年: 2025
畢業學年度: 113
語文別: 英文
論文頁數: 56
中文關鍵詞: 黃韌帶肥厚血管新生VEGF-ATSP-1膠原/彈性蛋白比例
外文關鍵詞: Ligamentum flavum hypertrophy, angiogenesis, VEGF-A, TSP-1, collagen/elastin ratio
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  • 摘要 I Abstract III 誌謝 V Contents VI List of Figures VIII List of Tables XI List of Abbreviations XII Chapter 1 Introduction 1 1.1 Background 1 1.2 Ligamentum flavum hypertrophy (LFH) 1 1.2.1 Dorsal and Ventral Differences in Ligamentum Flavum 3 1.2.2 Structure and Sprouting Angiogenesis 4 1.3 Aims of the research 5 Chapter 2 Materials and Methods 7 2.1 Experimental workflows 7 2.2 Materials 8 2.3 Recipes 8 2.4 LF tissue 9 2.5 Histology 11 2.5.1 Hematoxylin and Eosin (H&E) Staining 12 2.5.2 Elastic Van Gieson (EVG) Staining 13 2.5.3 Immunohistochemistry (IHC) Staining 14 2.6 Statistical analysis 15 Chapter 3 Results 17 3.1 LF Anatomical Views 17 3.2 Distribution of orientation and coherency in LF tissue 18 3.3 Collagen/Elastin ratio in LF tissue 21 3.4 The Relationship between Vessel Area and Nucleus Count 23 3.5 VEGF-A expression in LF tissue 26 3.6 TSP-1 expression in LF tissue 29 Chapter 4 Discussion 33 Chapter 5 Conclusion 36 Chapter 6 Future work 38 Reference 39

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