利用植物細胞核基因轉殖技術，發展家禽里奧病毒(avian reovirus；ARV)的可食性疫苗。隨著植物生物技術的發展，以植物生產動物疫苗可降低成本、可口服、較為安全，無人畜共通疾病、可表現於貯存組織運送方便等優點，許多以植物生產疫苗的研究，在動物試驗中證實是可引發動物的免疫反應，並可以對抗病原菌的感染。經由前人的研究，家禽里奧病毒之外鞘膜蛋白(outer capsid protein) σC可誘發中和抗體的產生，因此，我利用植物細胞核基因轉殖技術將S1基因分別轉殖至苜蓿與菸草之中，利用不同的轉殖載體使σC蛋白分別表現於苜蓿與菸草。在PCR的測試及GUS的測試，可證實家禽里奧病毒S1基因已融合於苜蓿及菸草的基因體之中；以西方墨點法除了得知σC蛋白質的確可表現在已轉殖的植株外，且可篩選出表現最高的轉殖植株，以此做為雞隻的可食性疫苗，希望可誘發雞隻產生中和抗體，並可抵抗家禽里奧病毒的感染。

　Several animal feeding experiments have shown that transgenic plant with over expression the surface antigen of pathogen can cause animal to induce immunity to against pathogen attack. In order to develop plant-based vaccine for poultry against avian reovirus, I have cloned S1 gene into plant nuclear expression vector pCAMBIA1301 and pCAMIBA2301. I also have transformed the expression constructs into both alfalfa and tobacco tissues by Agrobacteria-mediated transformation method. The alfalfa and tobacco transformants were selected with media containing antibiotics (hygomycin or kanamycin). PCR analysis and GUS histochemical assay have confirmed that the S1 gene have integrated into the chromosome of alfalfa and tobacco . transformantsI selected the transgenic lines with highest σC protein expression level by western blot analysis, and tested the immune response by injecting the plant–expressed σC protein to mice.

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