調配試劑的濃度比例和試劑的混合是生化實驗中兩個重要的步驟，調配試劑的濃度並使試劑混合均勻在藥物調配、樣品稀釋、化合物合成等實驗中都是重要的關鍵。本研究的目的是在微管道晶片中整合調配試劑濃度比例與試劑混合兩個過程。
根據過去研究發現，可藉由改變三維交錯管道交錯窗口之後的流阻比調控出口濃度比例;本研究設計一個三維交錯管道結合混合器的設計，藉由改變交錯窗口之後的流阻比產生預期的出口濃度比例，同時並提升混合均勻程度。
本研究由CFD-RC數值模擬軟體計算流場和流體混合效率。藉由數值模擬結果，確定本研究使用的管道外型並進行實驗。本研究利用影像分析軟體ImageJ分析實驗結果的光度值量測出口濃度比例。最後將實驗結果與模擬結果比對，兩者結果相符，確認了實驗符合數值模擬結果。
為了更進一步探討本研究結果是可行的，將本研究結果與過去研究比較，結果發現，管道流阻比與出口濃度比例的關係與過去研究推導的「濃度比例公式」相符，最後本研究探討了出口濃度比例與出口位置的關係，在不受製程條件的限制下，本研究所使用的管道外型可產生任何介於0~1的出口濃度比例，並藉由出口前混合器的設計提升混合指數。
本研究證明三維交錯微管道結合混合器的簡單外型設計可產生任何介於0~1之間的濃度比例。本研究的設計概念整合流體輸送、濃度比例控制、流體混合於一個外型簡單的管道晶片中實現。

Controlling the concentration ratio of reagent and the mixing of solution are two important processes in biochemical experiments. Controlling desired reagent ratio of solution and making it well-uniform are the keys in drug allocation, sample dilution, synthesis reagents and so on. The purpose of this study is to integrate controlling the concentration ratio and the mixing functions in a micro channel chip.
This study employs the three dimensional tangentially crossing micro channel. In the previous study, the flow channel resistance after the crossing window can affect the concentration ratio. This research devises a three dimensional tangentially crossing micro channel with a mixer. It is possible by controlling resistance of the branch channel to achieve the desired concentration ratio in the expected outlets. In addition, the outlet solution will be uniformly mixed.
The study utilized simulation software CFD-RC to calculate the flow and mixing characteristics. According to the simulation result, the result confirmed the configuration of PDMS micro channel chip for experiments. This study used software ImageJ to analysis the grey scale of the digital image to evaluate the concentration ratio .The results of experiments are in agreement with the numerical simulation.
To validate the design of the channel , this research compared the results with the previous research . It shows that the relation of concentration ratio and flow resistance is matched with the “concentration ratio formula” which was derived from the previous study.Finally, this research discussed the relation between the outlet location and the concentration ratio. Except the fabrication limit, this micro channel chip can generate any concentration ratio between 0 to 1.
This study demonstrated that three dimensional tangentially crossing channel combined mixer can generate any concentration ratio by simple configuration. This micro device integrates the functions of fluid transportation, reagent solution preparation, direction control and mixing in a single chip.

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