本研究結合適體(Aptamer)分子與紅外吸收光譜發展定量汞離子檢測系統，並利用無電鍍製程沉積奈米級島狀金膜於多晶矽半圓柱之平整表面上，藉以放大入射光訊號，並可有效降低檢測時的濃度限制。在過去20年，適體已廣泛被應用於各種領域，包含藥物開發、微生物檢測以及環境水質的即時監控，透過適當的序列設計，適體還可改變主鏈結構並與多種目標分子緊密結合。相較於單株抗體的成本昂貴以及使用與保存上的限制，適體具有可以大量生產、成本低廉以及穩定性高等優點；衰減式全反射型表面增顯紅外光技術(Attenuated Total Reflection-Surface Enhanced Infrared Absorption Spectroscopy, ATR-SEIRA)分析，由於具備高的靈敏度與時間解析度以及表面選擇性與低溶液背景干擾的特性，適合做為時變性介面研究。本實驗利用32個鹼基的單股去氧核醣核酸序列作為捕捉二價汞離子的專一性適體，並在5’端修飾有巰基六碳鏈與基板連接及3’端的亞甲藍分子作為光學上的特徵指示物，在適體與二價汞離子的反應後，適體分子的構型將由原本的鏈狀轉變為由中間對折的髮夾型(hairpin)，這同時使得末端的亞甲藍分子也從原本的遠離表面變成更接近表面，遂可以藉由觀察到的亞甲藍光譜特徵峰值之增減，作為定量汞離子的判斷基礎；利用此一特性並搭配電化學方法，可進一步確認了表面修飾的最佳濃度參數，以及後續的二價汞離子濃度對應特徵峰值的線性量測範圍和最低濃度檢測極限。

In this study, a quantitative mercuric ion detection system was developed with aptamer and infrared absorption spectroscopy. Nano-crystalline island-shaped gold film was deposited on polycrystalline silicon prism by electroless plating process to amplify signal of incident light and effectively lower the limit of detection. Aptamers have been widely used in a variety of fields including drug development in the last two decades, microbiological sensing and environmental monitoring through proper sequence design. Compared with the cost and restrictions while using or preserving of monoclonal antibody, aptamer has many advantages such as mass production by PCR, low cost and high stability. The attenuated total reflection surface-enhanced infrared absorption spectroscopy (ATR-SEIRAS) was used to investigate due to high sensitivity, high resolution of time, surface selectivity and low solution background interference. In this research, 32-mer of single-stranded
DNA sequence was used as the specific aptamer to capture mercuric ions. Methylene blue (MB) was linked at 3’ end of aptamer as optical indicator and mercaptohexane (MCH) was immobilized at the opposite end as a linker with gold. The configuration of aptamer will transform into hairpin shape after combining with Hg(II), so that methylene blue will move from bulk to surface. With this feature, the optimal parameters of surface aptamer modification and corresponding linear detection range / limit of detection were further confirmed.

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