由於氣候變遷及自然災害發生更加頻繁，現今環境保護相關主題被認為是與經濟發展同等重要。在環境相關領域裡，降低二氧化碳排放使全球暖化得以舒緩是一項迫切需要著手處裡的議題，在所有二氧化碳捕捉及固定技術中，透過微生物產生能源來補足能源需求漸增的世代，同時藉由光合作用固定二氧化碳達到降低碳排放的生質能生產結合碳捕集儲存技術 (BECCS)，被視為是最具發展潛能的技術。
除了碳排放議題外，近年來對於豬肉需求大增而使養豬場紛紛擴建，造成更大量的養豬廢水產生也是一項需要被關注的問題。養豬廢水為大眾所熟知的含有高量的 BOD、COD、氨氮及微量汙染物質，需經過妥善的處理才可排放或再利用於灌溉。由於很多的農地使用豬糞尿施肥或養豬廢水灌溉被發現受到重金屬汙染而越來越受到重視，特別是金屬鋅及銅最常被檢出，因為在促進豬隻生長及增強免疫力的飼料添加劑中常含有此兩種重金屬。為此，有大量的研究提出利用藍綠菌或藻類可以有效降解水中重金屬，但大部分都使以合成培養基進行實驗而非利用養豬原廢水進行實驗。
在本研究中，選擇 Thermosynechococcus sp. CL-1 (TCL-1) 作為處理養豬廢水與煙道後段濕式洗滌塔吸收液混合之廢水，因其具有耐高溫及高鹼性環境適應的能力，且以 TCL-1 培養於所設計的生物反應器內，預期可達到同時降低二氧化碳排放及去除含有重金屬汙染物之廢水的目標。評估在不同的光照強度下培養，對於固碳效率、重金屬去除率、生質能潛能 (醣類、脂質、蛋白質)的影響。研究結果顯示，最佳的生質體產率為55.69 ± 6.17 mg/L/h 及最佳的固碳速率為 97.90 ± 6.17 mg/L/h，均發生在光照強度 1,000 μE/m2/s 之培養條件下，而最高的重金屬鋅去除率達到 62.5 ±8.8%，也發生在光照強度 1,000 μE/m2/s 之培養條件下。根據副產物組成分分析，TCL-1 含有高量的蛋白質，大約占細胞乾重 60-70%，適合作為飼料、營養食品原料或運用於製藥產業。此外，TCL-1培養於廢水中可以刺激醣類的合成，經過 12 小時培養後可觀察到細胞內醣類含量增加至 10.8 ± 0.4%及最高的醣類產率為 9.4 ± 1.0 mg/L/h。

The subjects of environmental protection are concerned as important as economic development in this modern time since the climate change and disaster happened more frequently. One of the urgent actions take part in environment sector is reducing CO2 emission to control global warming. Among all CO2 capture and sequestration technology, bioenergy with carbon capture and storage (BECCS) is considered as a promising technology which produces energy by microorganism to meet the rising demand of energy and cut down the CO2 emission through photosynthesis process.
In addition to CO2 emission, the increasing demand of pork has caused much more amount of swine wastewater being generated by the expansion of pig farm nowadays. Swine wastewater is well known for rich in high BOD, COD, ammonium and some micropollutant that require properly treated before irrigational reuse or discharge. It receives rising attention about the appearance of metals as one of micropollutant have polluted farmland through reuse of pig manure or recycled swine wastewater for irrigation, especially zinc and copper are the most determined heavy metal which is the ingredient of supplement for pig growth and illness treatment. In this regard, the prominent performances of heavy metal degraded by cyanobacteria/microalgae have been proposed, but there were still few studies have been conducted under real wastewater cultivation.
Thermosynechococcus sp. CL-1 (TCL-1) was selected for treating a mixture of real swine wastewater and simulated alkaline solution of chemical wet scrubber in this study with its characteristics of high temperature tolerance ability and alkaline aqueous adaptation. The designed TCL-1 based bioreactor is expected to have ability of reducing CO2 emission and removing pollutants including heavy metal in the wastewater at the same time. The performances were tested under various light intensities and the bioenergy production potential by the contents of lipid, protein, and carbohydrates in biomass was also assessed. The results show that the optimal biomass productivity and CO2 fixation rate in wastewater cultivation are 55.69 ±6.17 mg/L/h and 97.90 ± 6.17 mg/L/h, respectively, under 1,000 μE/m2/s light intensity cultivation condition. The highest zinc removal rate can reach to 62.5 ±8.8%, which is also achieved under 1,000 μE/m2/s light intensity cultivation condition. According to the byproducts analysis, TCL-1 is considered to be a protein-rich cyanobacteria as its protein content is around 60-70% dry weight, suitable for producing feed, supplement and applying in pharmaceutical industry. Moreover, cultivated TCL-1 with wastewater can stimulate carbohydrate production, result in higher carbohydrate content and carbohydrate productivity of 10.8 ± 0.4% and 9.4 ± 1.0 mg/L/h after 12 hours cultivation.

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