本論文以化學還原法將金奈米粒子綴飾於聚丙烯酸(PAA)被覆之多壁奈米碳管(MWCNTs)表面，並將其沉積在網印碳電極上，用於葡萄糖與過氧化氫的電化學感測。首先將奈米碳管加入PAA水溶液中，在超音波下均勻混合形成PAA被覆之奈米碳管，並以聯胺為還原劑，將金奈米粒子綴飾於其表面。接著，在網印碳電極表面沉積此溶液，再進行葡萄糖與過氧化氫的感測。結果顯示，經Au-PAA-MWCNTs修飾之網印電極，在磷酸鹽緩衝溶液(0.1 M、pH 7.5)與氫氧化鈉溶液(0.1 M)中，對葡萄糖與過氧化氫皆有良好的電催化活性。在磷酸鹽緩衝溶液(0.1 M、pH 7.5)與氫氧化鈉溶液(0.1 M)中，以循環伏安法偵測葡萄糖濃度的線性範圍分別為0.5~30 mM與0.5~40 mM，偵側極限分別為84μM和37μm；以計時安培法偵測葡萄糖濃度的線性範圍則分別為2~10 mM、10~20 mM、20~46 mM與2~20 mM、20~50 mM，偵側極限分別為80μM和20μM。此外，在磷酸鹽緩衝溶液(0.1 M、pH 7.5)中，以安培法偵測過氧化氫，其濃度線性的範圍為10~1000 μM，偵側極限為3.84 μM。

In this thesis, Au nanoparticles were decorated on PAA-coated multi-walled carbon nanotubes (MWCNTs) via chemical reduction and then deposited on the screen printed carbon electrode for the electrochemical detection of glucose and hydrogen peroxide. Firstly, MWCNTs were well dispersed into the aqueous solution of PAA via sonication to yield the PAA-coated MWCNTs, and then Au nanoparticles were decorated on their surface using hydrazine as the reducing agent. Secondly, the screen printed carbon electrode was modified with the resulting solution and then used to detect glucose and hydrogen peroxide. The result revealed that the screen printed carbon electrode modified by Au-PAA-MWCNTs displayed excellent electrocatalytic activities towards glucose and hydrogen peroxide in phosphate buffer solution (0.1 M, pH7.5) and NaOH solution (0.1 M). In phosphate buffer solution (0.1 M, pH7.5) and NaOH solution (0.1 M), the linear calibration curves of glucose concentration obtained by cyclic voltammetry were in the ranges of 0.5~30 mM and 0.5~40 mM, respectively. Their detection limits were 84μM and 37μM, respectively. The linear calibration curves of glucose concentration obtained by amperometric response were in the ranges of 2~10 mM, 10~20 mM, and 20~46 mM for phosphate buffer solution system and 2~20 mM and 20~50 mM for NaOH system. Their detection limits were 80μM and 20μM, respectively. Moreover, in phosphate buffer solution (0.1 M, pH7.5), the linear calibration curve of hydrogen peroxide concentration obtained by amperometric response was 10~1000 μM with a detection limit of 3.84 μM.

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