微拉伸量測系統是研究生物細胞和組織的力學特性重要工具，本研究的目的在於建構微拉伸量測系統和量測方法，以量測C2C12肌纖維母細胞和PC-12類神經細胞受到拉伸時的被動機械性質。藉由有限元素軟體Abaqus建構細胞模型，將細胞假設為線性彈性材料，模擬細胞受拉伸時的狀態，以獲得細胞之楊氏模數，且瞭解細胞受拉伸時內部的應力分佈。
由結果顯示，兩種細胞實驗量測和有限元素模擬出的楊氏模數之數量級皆與文獻相符，實驗方面C2C12細胞視楊氏模數約為4.98kPa，PC-12細胞視楊氏模數約為1.27kPa，有限元素模擬單層結構方面C2C12楊氏模數為12.19kPa，PC-12楊氏模數為1.86kPa。有限元素模擬雙層結構方面C2C12楊氏模數核區為13.50kPa，細胞骨架區為11.03kPa，PC-12楊氏模數核區為2.12kPa，細胞骨架區為1.54kPa。成功發展微拉伸量測系統量測細胞視楊氏模數，及有限元素模擬獲得細胞楊氏模數，且能表現出活體細胞的被動機械特性。

Micro-tensile testing system is important for studying the biomechanics of cells and biological tissues. The goal of this thesis is to construct a micro-tensile testing system and measurement method. The built system is employed to measure the passive mechanical properties of C2C12 myoblast cells and PC-12 neuron-like cells. By using finite element method, numerical models of the cells are built by assuming the cell material to be isotropic and linearly elastic. Young’s moduli of the cells are estimated by simulating stretching of the cells with the FEM models to explore intracellular stress distribution.
The results show that orders of the magnitude of Young’s moduli of these two cells obtained from experiments and FEM are consistent with those of the literature. The apparent Young’s moduli of C2C12 cells and PC-12 cells obtained from the experiments are 4.98Pa and 1.27kPa respectively. The Young’s moduli of C2C12 cells and PC-12 cells obtained from single-layer FEM simulations are 12.19kPa and 1.86kPa respectively. The nucleus and cytoskeleton Young’s moduli of C2C12 cells and PC-12 cells obtained from double-layer FEM simulations are 13.50kPa,11.03kPa and 2.12kPa,1.54kPa respectively.
In conclusion, methods for measuring the apparent Young’s modulus of cell by using a micro-tensile testing system and linear elastic finite element method of cell are developed. The methods can be utilized to characterize the passive biomechanical properties of living cells.

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