陰陽離子界面活性劑(Catanionic surfactant)，又稱為離子對雙親分子(Ion-pair amphiphile, IPA)，為本研究中主要材料之一，由於具有豐富的來源、高度的化學穩定性，分子結構類似脂質，且價格較為低廉，因此為藥物傳輸載體的理想選擇。本研究中以陰陽離子界面活性劑DeTMA-TS(Decyltrimethylammonium-tetradecylsulfate, C10-C14)，透過強制製程方式，添加不同比例的膽固醇(XCHOL=0~0.5)和乙醇(EtOH=0~20 vol%)，形成類乙醇體陰陽離子液胞分散液，探討液胞粒徑和雙層膜堅硬度之間的關聯性，並驗證強制製程液胞形成的機制。
實驗結果顯示，不同乙醇和膽固醇濃度所組成的液胞其溫度對製備完後的液胞粒徑影響不大。進一步將雙層膜堅硬度與粒徑做關聯性分析，實驗結果顯示無論是螢光偏極化技術還是傅立葉轉換紅外光譜儀，所得到的雙層膜堅硬度與粒徑呈現線性相關，這為強制製程液胞形成機制提供了有力的證據。然而，兩種儀器所得到的相關性存在差異，推測由於儀器測量原理的不同所導致的。傅立葉紅外光譜儀利用分子的振動評估碳氫鏈的整體秩序度，然而螢光偏極化技術受限於螢光探針結構的限制，乙醇濃度差異對雙層膜的影響隨著碳氫鏈的位置有所差異，因此無法完整表達乙醇對雙層膜的影響。最後，透過標準化的方式探討膽固醇與乙醇對物理性質的影響，由結果可知，透過FP測量的雙層膜堅硬度表示乙醇的效應遠低於膽固醇，而透過FT-IR測定得到的雙層膜堅硬度結果表示乙醇與膽固醇的效應差異不大，這與膽固醇和乙醇對粒徑的影響相似。因此，透過傅立葉轉換紅外光譜儀能更完整地呈現類乙醇體陰陽離子液胞中雙層膜分子的排列情況。

In this study, the catanionic surfactant DeTMA-TS (decyltrimethylammonium-tetradecylsulfate, C10-C14) was used to fabricate ethosome-like catanionic vesicle by forced formation process was by adding varying proportions of cholesterol (XCHOL = 0~0.5) and ethanol (EtOH = 0~20 vol%). Effects of CHOL and EtOH on the physical properties of vesicle were investigated. Furthermore, the relationship between vesicle size and bilayer rigidity was established with the aim to validate the mechanism of vesicle formation. The correlation analysis between bilayer rigidity and vesicle size revealed a linear relationship regardless of whether it was assessed by fluorescence polarization (FP) technique or Fourier-transform infrared (FT-IR) spectroscopy, respectively. However, there were differences in the correlations obtained from the two instruments, which is attributed to the different measurement principles of the instruments. In addition, the data obtained from the FP technique and FT-IR spectroscopy were compared, and the results were further analyzed for differences. The reason of the differences was that FT-IR spectroscopy is used to measure the vibration of molecules and detect the overall bilayer rigidity. On the other hand, the FP technique is limited by the structure of the fluorescence probe (DPH), resulting in more difficult to assess the effects of ethanol on the bilayer membrane. The results showed that EtOH and CHOL effects on bilayer rigidity by FT-IR spectroscopy was similar on vesicle size, indicating a higher correlation when using FT-IR results. Therefore, FT-IR spectroscopy can provide a more comprehensive representation of the molecular arrangement within bilayer membrane of ethosome-like catanionic vesicles.

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