塑膠微粒是廣泛存在於不同基中的有毒物質，需要先更精確掌握檢測和計數方法，才能進一步減少及防止其散佈至環境中，故了解哪種基質能幫助辨別自來水廠中的塑膠微粒是相當重要的一環。本研究旨在研究尼羅紅 (Nile Red) 染料在塑膠微粒分析中的適用性，以及建立過濾樣品的最佳化條件，並減少有機物和樣品染色後對檢測的影響。研究結果顯示，經聚碳酸酯蝕刻濾膜過濾並使用5 mg/L溶解於正己烷的尼羅紅染色後，能夠在460-495 nm的波長下輕易辨別出塑膠微粒，10µm 和 150 µm的塑膠微粒樣品回收率分別為 93% 和 85%。在樣品中添加10 mg/L 次氯酸鈉3小時和 25% 過氧化氫 21小時組合的前處理能有效降低樣品中有機物質和螢光物質的干擾。本研究同時將建立的樣品處理方法和檢測方法應用於台灣的三個自來水廠中，並在三個水廠的原水及清水中均發現塑膠微粒的存在，濃度分別為 165-195 和 100-150 個塑膠微粒/升。 清水中的塑膠微粒主要的尺寸範圍為1-10 μm 和 10-50 μm，其中51-64% 呈碎片狀。本研究中所建立的方法可做為未來監測塑膠微粒之參考。此外，在自來水廠中檢測到的塑膠微粒濃度亦顯示台灣的自來水廠需要進行全面性的詳細監測和研究。

Microplastics (MP) are toxic materials found in different substances, and environmental engineers need to have a proper grasp of microplastic count and exploration, to know how to lessen and prevent their proliferation. This became a preliminary catalyst for the pursuit of this study, to check what medium could help identify microplastics easily, with the main goal of detecting microplastics in Drinking Water Treatment Plants (DWTPs). Hence, this study is aimed to investigate the applicability of the Nile Red (NR) dye for microplastic analysis, to establish the optimal conditions for membrane types for sample filtration, and reduction of the effect of organic matters and sample staining for detection. Experimental results indicated that in the samples filtrated by polycarbonate track-etch membrane and stained with 5 mg/L of NR dissolved with n-hexane, MPs were easily recognizable when viewed under the excitation wavelength of 460-495nm. The recovery of MPs in the samples was at 92.96% and 84.57% for the sizes of 10 and 150 µm respectively. The samples pretreated with 10 mg/L NaOCl for 3 hrs and 25% H2O2 for 21 hrs showed a 92.07% decrease in organic material compared to an 89.07% reduction using only 25% H2O2 for 24 hrs. The Raman spectroscopy analysis of Cheng Kung Lake waster samples further confirmed that the combination of NaOCl and H2O2 treatment effectively reduced the fluorescent interference on MP detection caused by the non-MP presence in the samples. The developed sample treatment and detection method was applied in the measurement of MPs in three DWTPs in Taiwan. MPs were found in both raw and finished water in three DWTPs, with a total number of 165-195 and 100-150 microplastic particles, respectively. The microplastics present in the finished water samples were dominated by the size ranges of 1-10 and 10-50 μm and with 51-64% of particles being fragmented shape. The method developed in this study may serve as a reference if MPs are to be detected in DWTP. In addition, the MP concentration detected in DWTPs suggested that a further larger scale and more detailed monitoring study is needed for Taiwan's DWTPs.
In addition, the MP concentration detected in DWTPs suggested that a further larger scale and more detailed monitoring study is needed for Taiwan’s DWTPs

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