神經導管的使用在治療神經損傷的病患中扮演著輔助再生角色，因此，如何提升管內斷端神經再生效率一直是研究的重點。本研究將從一般軸突生長的角度出發，重點在於觀察軸突生長過程中的生理和生物力學變化。
本研究實驗樣本為PC-12類神經細胞，藉由添加神經生長因子(NGF)誘發軸突生長，紀錄軸突在ㄧ般情況下的生長過程，並針對給予電場刺激的情況下，觀察電場因素對軸突生長的影響。而後搭配原子力顯微鏡，利用壓深實驗探測軸突生長過程中黏彈力學特性的變化，並量測軸突各區域在不同基質下貼附力的差異。
實驗結果顯示軸突生長分期中與細胞長度及分支數目具有相關性，同時彈性係數於生長初期時為0.98±0.48 kPa；中期為1.27±0.58 kPa；末期則為2.43±1.01 kPa，呈現增加的趨勢。此外，軸突中端貼附力(3.79±1.63 nN)小於軸突近端(16.38±2.43 nN)及生長錐(11.68±1.76 nN)部份，且藉由塗覆膠原蛋白基質可有效增加軸突本身貼附性質。

The use of nerve conduit plays an important role on auxiliary regeneration during the therapy of injured peripheral nerve. How to improve the efficiency of axon regeneration of the injured peripheral nerves in the nerve conduit is an important issue. This study focuses on normal axon to observe the biological and biomechanical variations during the growth process.
PC-12 neuron-like cell is the experimental subject and the axon growth process was recorded by adding Nerve Growth Factor (NGF) to induce axon growth. The effect of electric fields stimulation was also observed. To utilize Atomic Force Microscopy (AFM), the viscoelastic properties of axon were measured by using indentation test during the growth process and the difference of adhesion force on axon was also measured.
The results show that axon growth process has correlation with cell body length and branch number, the increasing trend of elastic modulus during axon growth process is also presented (initial stage: 0.98±0.48 kPa, middle stage: 1.27±0.58 kPa, last stage: 1.27±0.58 kPa). Furthermore, the adhesion force on the middle axon (3.79±1.63 nN) is smaller than the proximal axon (16.38±2.43 nN) and growth cone (11.68±1.76 nN) and adhesion force can be increased by coating collagen as the substrate.

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