本研究以單碳鏈陽離子型界面活性劑 hexadecyltrimethylammonium bromide (HTMAB) 及單碳鏈陰離子型界面活性劑 sodium dodecylsulfate (SDS) 所形成之雙碳鏈離子對雙親分子 hexadecyltrimethylammonium-dodecylsulfate (HTMA-DS)為主材料，添加雙鏈的陽離子型界面活性劑 dihexadecyldimethylammonium bromide (DHDAB) 及膽固醇，採用強制型製程製備出陰陽離子液胞，並分析膽固醇含量對於液胞之物理特性、相轉移溫度，以及雙層膜流動性的影響。此外，利用水溶性高分子玻尿酸 (hyaluronic acid, HA)修飾陰陽離子液胞，探討不同含量玻尿酸對於液胞物理特性、雙層膜流動性，以及玻尿酸與液胞結合行為的影響，且進行維他命E醋酸酯的包覆實驗，以探討液胞經玻尿酸修飾後對於藥物包覆行為的影響，並由細胞存活度檢測、溶血實驗評估載體應用的可行性。
實驗結果顯示，純成分HTMA-DS無法形成穩定的液胞結構，需要添加DHDAB提供液胞之間的電荷斥力，以及添加膽固醇調節雙層膜的排列，才能形成穩定的液胞結構。此外，關於添加膽固醇對液胞雙層膜流動性的影響，依照相轉移溫度作區隔會規則效應(order effect)及不規則效應(disorder effect)出現，當膽固醇含量高於20 mol%時，液胞雙層膜結構的相轉移現象幾乎消失。
在玻尿酸修飾液胞的實驗中，玻尿酸可以靜電吸引的方式吸附於液胞表面，因此玻尿酸添加量上升使得液胞界面電位下降，甚至造成電性反轉。就初始平均粒徑而言，玻尿酸的吸附會造成液胞粒徑上升，當界面電位接近等電點時，則有明顯的液胞聚集及沉澱。螢光偏極化法及紅外光譜法的分析結果指出，玻尿酸的吸附對於液胞雙層膜流動性及分子碳氫鏈排列沒有造成明顯的改變，表示玻尿酸的吸附並未深入到液胞雙層膜區域。
此外，藥物包覆的實驗指出，液胞經由玻尿酸修飾並包覆維他命E醋酸酯後，物理性質並沒有明顯變化，在適當的玻尿酸濃度下，穩定的液胞結構能夠有80 %以上的包覆效率。針對液胞的應用可行性評估，以人類非小肺癌細胞株及人類胚胎腎細胞株進行生物活性的檢測，並利用溶血實驗分析液胞對紅血球的影響，結果顯示當液胞濃度低於0.25 mM 時，細胞的存活率即高於80%，可視為低毒性。但是經玻尿酸修飾後之液胞的細胞存活性有下降的傾向，可能因為所採用玻尿酸(1.5-1.75 MDa)屬於高分子量級別(>500 kDa)，而抑制細胞的生長。

In this study, the double-chained ion pair amphiphile, hexadecyltrimethylammonium-dodecylsulfate (HTMA-DS), was prepared from the corresponding mixed cationic/anionic surfactant system. In order to investigate the properties of the vesicle/HA complexes, the double-chain cationic surfactant, dihexadecyldimethylammonium bromide (DHDAB), and cholesterol were used as additives to fabricate the positively charged catanionic vesicles with high physical stability. By the forced formation process, HTMA-DS/DHDAB/cholesterol with 43 mol% cholesterol had high physical stability. In addition, the zeta potential of the vesicles was increased with increasing the DHDAB content, and the initial size of the vesicles was not affected by the DHDAB content. The membrane fluidity and phase transition temperature were analyzed by fluorescence polarization (FP) technique. With increased cholesterol content, the phase transition behavior of the vesicular bilayers gradually disappeared. Furthermore, cholesterol could induce both disordering and ordering effects on the vesicular bilayer fluidity in the different regions of temperature. With the consideration of targeted drug delivery applications, the vesicles were modified by hyaluronic acid (HA) to improve anticancer therapy and biopharmaceutical properties. The vesicles and HA possessed opposite charges, and HA could adsorb on the vesicle surfaces to form the vesicle/HA complexes. The zeta potential of the complexes was decreased with the increase in the HA content, and the charge character of the complexes was even reversed at high HA contents. Furthermore, association of the catanionic vesicles and HA increased the initial size of the vesicles. The analyses of FP and FTIR indicated the association of the vesicles and HA caused no significant change in the vesicular bilayer fluidity and the conformational freedom of molecular hydrocarbon chains. For drug carrier delivery applications, the vesicle/HA complexes could successfully encapsulate the hydrophobic material, vitamin E acetate. In addition, H1299 and HEK293 cells were used to perform the cytotoxicity assay for the vesicle/HA complexes. The cell viability and hemolysis ratio could be improved by reducing the vesicle concentration. However, cell viability for the vesicle/HA complex was reduced because the high molecular weight of HA would inhibit the cell growth.

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