成功大學博碩士論文系統

簡易檢索 / 詳目顯示

回結果列表

研究生：	米瑞緹 Meethonganantamas Jiratorn
論文名稱：	調控植物缺磷逆境下膜脂質重塑之轉錄因子的功能性分析 Identification of Transcription Factors Regulating Phosphate Starvation Responses focusing on Membrane Lipid Remodeling in Plants
指導教授：	葉顓銘 Yeh, Chuan-Ming 蔡文杰 Tsai, Wen-Chieh
學位類別：	碩士 Master
系所名稱：	生物科學與科技學院 - 熱帶植物與微生物科學研究所 Institute of Tropical Plant Sciences and Microbiology
論文出版年：	2022
畢業學年度：	110
語文別：	英文
論文頁數：	78
中文關鍵詞：	轉錄因子、植物缺磷胁迫下、拟南芥、水稻、膜質重塑之
外文關鍵詞：	Transcription factors, Phosphate starvation in plants, Arabidopsis, Rice, Membrane lipid remodeling
相關次數：	點閱：99 下載：0
分享至:	分享至facebook 分享至twitter

查詢本校圖書館目錄查詢臺灣博碩士論文知識加值系統勘誤回報

Phosphate (Pi) is an essential macronutrient for plant and its availability is a major factor limiting plant development and productivity. Plant responds to low availability of Pi by networks of developmental and metabolic adaptation which then sustain plant growth and survival. One of the plants responding mechanisms to low external Pi is to alter phospholipids of cell membrane and release Pi as an internal Pi source. Many studies revealed that hundreds of genes are either induced or repressed as plant is responding to Pi starvation, some of which include the genes involved in membrane lipid remodeling. But the molecular mechanism that regulates these genes expression during phosphate starvation remains poorly understood. This study aims to identify transcription factors (TFs) which are involved in membrane lipid remodeling in response to Pi starvation. Yeast one-hybrid (Y1H) screening was performed with the promoter of genes involved in membrane lipid remodeling using Arabidopsis transcription factor-only cDNA library. We isolated 13 transcription factors that interact with the promoter of NON-SPECIFIC PHOSPHOLIPASE C4 (NPC4), and six of them also appeared to interact with SULFOQUINOVOSYLDIACYLGLYCEROL 2 (SQD2) promoter. The 13 TFs were individually investigated using bioinformatics tools and gene expression analysis, resulting in a NAC family TF, NAC038, the most promising candidates out of the 13 TFs, being preferentially studied further. Furthermore, both NAC038 and the TF which is evolutionary closely related to NAC038, NAC058, were up-regulated under Pi-deficiency in both Arabidopsis and rice, and were also found co-expressing with NPC4. Later, luciferase reporter assay will be utilized to confirm the interaction of the TFs to the NPC4 promoter in Arabidopsis. To ascertain these chosen TFs regulatory function over downstream genes, gene expression is being analyzed in the nac038 and nac058 mutants, overexpression and CRES-T (Chimeric REpressor gene Silencing Technology) transgenic lines. From NPC4 expression analysis using nac038 mutant and 35SNAC038:VP16 transgenic lines, NAC038 was suggested to be a negative regulator of NPC4 and work to suppress NPC4 especially under Pi deficiency.

Contents
Acknowledgement…………………………………………………………………………………...I
Abstract……………………………………………………………………………………………...II                                                         
Contents…………………………………………………………………………………………….IV
Lists of Figures……………………………………………………………………………………....V
Lists of Tables…………………………………………………………………………………….....V
Lists of Supplementary Figures……………………………………………………………….........VI
Lists of Supplementary Tables……………………………………………………………………...VI
Lists of Supplementary data……………………………………………………………………..…VI
Abbreviations...................................................................................................................................VII
1. Introduction 
1.1 Phosphorus crisis…………………………………………………………………………….......1
1.2 Plant phosphate starvation responses………………………………………………………..…...2
1.3 Membrane lipid remodeling upon Pi starvation…………………………………………….....…4
1.4 High efficiency yeast one-hybrid screening………………………………………………….......6
1.5 Chimeric Repressor gene Silencing Technology (CRES-T) transgenic plants..............................7
2. Aim of study…………………………………………………………………………………..…...9
3. Materials and methods
3.1Plant materials and handling…………………………………………………………………….10
3.2 DNA extraction, purification and ligation……………………………………………………....11
3.3 Polymerase chain reaction (PCR)………………………………………………………………12
3.4 Bacteria strain and handling…………………………………………………………………….13
3.5 Cloning………………………………………………………………………………………….14
3.6 Yeast strain and handling……………………………………………………………………….14
3.7 Yeast one-hybrid (Y1H) screening……………………………………………………………...15
3.8 RNA extraction and reverse transcription………………………………………………………16
3.9 Real-time quantitative polymerase chain reaction (qRT-PCR)………………...……………….17
3.10 Phylogenetic tree construction…………………………………………………………….…..17
3.11 Gene expression analysis……………………………………………………………………...18
4. Results
4.1 Yeast one-hybrid screening for transcription factors interacting with NPC4 promoter……..…19
4.2 Arabidopsis NAC038 and NAC058 and their rice orthologs are up-regulated……………..….20
upon Pi starvation.
4.3 Expression analysis of NPC4 in the nac038 mutants and 35S:NAC038:VP16………………..23
  transgenic plants for characterization of NAC038
4.4 Yeast one-hybrid screening for transcription factors interacting with MGD3 and..…………...24
  SQD2 promoters
5. Discussion
5.1 TFs isolated from Y1H screening using NPC4 promoter as bait……………………………....26
5.2 NAC038 and NAC058 potentially affect membrane lipid remodeling upon Pi starvation……..27
5.3 TFs isolated from Y1H screening using MGD3 and SQD2 promoters as bait…………………29
5.4 The NPC4 promoter and SQD2 promoter were bound by 6 common TFs in yeast……… …...29
6. Conclusion and perspectives……………………………………………………………………..31
7. Reference………………………………………………………………………………………...33
Lists of Figures
Figure 1. Promoters cloned for Y1H experiment……………………………………………...…...40
Figure 2. Phylogenetic tree of NAC proteins of Arabidopsis and rice………………………......…41
Figure 3. The level of expression of NPC4, NAC038 and NAC058……………………………......42
  in developmental tissues of Arabidopsis
Figure 4. The level of expression of NPC4, NAC038 and NAC058……………………………......43
  in Arabidopsis under abiotic stresses
Figure 5. Quantitative real-time PCR analysis of gene expressions of NPC4, NAC038…………...44
  and NAC058 in Arabidopsis thaliana under Pi deficiency
Figure 6. Quantitative real-time PCR analysis of gene expressions of NPC4, NAC038…………...45
  and NAC058 in Oryza sativa under Pi deficiency
Figure 7. NPC4 expression in Arabidopsis nac038 mutant and 35S:NAC038:VP16 lines…...……47
Lists of Tables
Table 1. Y1H results using MGD3 promoter as bait………………………………………………..48
Table 2. Analysis of thirteen TFs isolated from Y1H screening…………………………………....50
  using NPC4 promoter as bait
Table 3. Y1H results using MGD3 promoter as bait………………………………………………..51
Table 4. Analysis of Forty-three TFs isolated from Y1H screening………………………………..55 
  using MGD3 promoter as bait                        
Table 5. Y1H results using SQD2 promoter as bait………………………………………………...57
Table 6. Analysis of twenty-eight TFs isolated from Y1H screening………………..……………..60
 using SQD2 promoter as bait
Table 7. Six TFs interacting with both NPC4 and SQD2 promoters in Y1H screening……………62
Lists of Supplementary Figures
Supplementary Figure 1. Diagram representing pathways of membrane lipid remodeling……......63
Supplementary Figure 2. 3-AT concentration test for Y1H screening………………………….......64
Supplementary Figure 3. Gene expression eFP Browser maps of Arabidopsis…………………….66
Supplementary Figure 4. Transcription factor binding motifs (TFBMs)…………………………...67
  of NAC038 and NAC058
Lists of Supplementary Tables
Supplementary Table 1. Lists of primers for cloning………………………………………...……..68
Supplementary Table 2. Lists of NAC proteins in phylogenetic trees……………………………...69
Supplementary Table 3. Lists of primers for qRT-PCR…………………………………………….70
Supplementary Table 4. T-DNA insertion mutants of Arabidopsis thaliana……………………….71
  used in this work
Supplementary Table 5. Primers for genotyping T-DNA insertion mutants………………………..71
Lists of Supplementary Data
Supplementary Data 1. Sequence of NPC4 (AT3G03530) promoter for Y1H screening………….72
Supplementary Data 2. Sequence of MGD3 (AT2G11810) promoter for Y1H screening…………73
Supplementary Data 3. Sequence of SQD2 (AT5G01220) promoter for Y1H screening……….…74
                                    

Vance CP, Uhde-Stone C, Allan DL (2003) Phosphorus acquisition and use: critical adaptations by plants for securing nonrenewable resource. New Phytol. 157: 423–447.
Vance CP (2001) Symbiotic nitrogen fixation and phosphorus acquisition. Plant nutrition in a world of declining renewable resources. Plant Physiol. 127: 390–397.
Roy ED, Richards PD, Martinelli LA, Coletta LD, Lins SRM, Vazquez FF, Willig E, Spera SA, VanWey LK, Porder S (2016) The phosphorus cost of agricultural intensification in the tropics. Nat Plants. 2
Sattari SZ, Bouwman AF, Martinez Rodriguez R, Beusen AHW, vanIttersum MK (2016) Negative global phosphorus budgets challenge sustainable intensification of grasslands. Nat. Commun. 7.
Schroder JJ, Cordell D, Smit AL, Rosemarin A (2010) Sustainable use of phosphorous. Plant Research International. 461.
Markus H, Daiju N, Gernot K (2014) Global Availability of Phosphorus and Its Implications for Global Food Supply: An Economic Overview. Kiel Working Paper No. 1897.
Cordell D and White S (2014) Life’s bottleneck: sustaining theworld’s phosphorus for a food secure future. Annu. Rev. Environ. Resour. 39: 161–88
Joosse PJ, and Baker DB (2011) Context for re-evaluating agricultural source phosphorus loadings to the great Lakes. Can. J. Soil Sci. 91: 317–327.
Reid K, Schneider K, McConke B (2018) Components of Phosphorus Loss From Agricultural Landscapes, and How to Incorporate Them Into Risk Assessment Tools. Front. Earth Sci. 6: 135
Zhang Z, Liao H, Lucas WJ (2014) Molecular mechanisms underlying phosphate sensing, signaling, and adaptation in plants. J Integr Plant Biol. 56(3): 192-220.
Peret B, Desnos T, Jost R, Kanno S, Berkowitz O, Nussaume L (2014) Root architecture responses: in search of phosphate. Plant Physiol. 166: 1713-1723
López-Arredondo DL, Leyva-González MA, González-Morales SI, López-Bucio J, Herrera-Estrella L (2014) Phosphate nutrition: improving low-phosphate tolerance in crops. Annu. Rev. Plant Biol., 65: 95-123.
Koyama H, Okawara R, Ojima K, Yamaya T (1988) Re-evaluation of characteristics of a carrot cell line previously selected as aluminum-tolerant cells. Physiol. Plant. 74: 683–687.
Wua L, Kobayashia Y, Wasaki J, Koyama H (2018) Organic acid excretion from roots: a plant mechanism for enhancing phosphorus acquisition, enhancing aluminum tolerance, and recruiting beneficial rhizobacteria. SOIL SCIENCE AND PLANT NUTRITION. 64(6): 697–704
Plaxton WC, Tran HT (2011) Metabolic Adaptations of Phosphate-Starved Plants. Plant Physiology. 156(3): 1006–1015.
Johri AK, Oelmüller R, Dua M, Yadav V, Kumar M, Tuteja N, Varma A, Bonfante P, Persson BL, Stroud RM (2015) Fungal association and utilization of phosphate by plants: success, limitations, and future prospects. Front Microbiol. 6: 984.
Lin W, Lin S, Chiou T (2009) Molecular regulators of phosphate homeostasis in plants. J Exp Bot. 60(5): 1427-38.
Kliebenstein DJ (2004) Secondary metabolites and plant/environment interactions: a view through Arabidopsis thaliana tinged glasses. Plant Cell Environ 27: 675–684.
Jiang C, Gao X, Liao L, Harberd NP, Fu X (2007) Phosphate Starvation Root Architecture and Anthocyanin Accumulation Responses Are Modulated by the Gibberellin-DELLA Signaling Pathway in Arabidopsis. Plant Physiology. 145(4): 1460–1470.
Naumann C, Müller J, Sakhonwasee S, Wieghaus A, Hause G, Heisters M, Bürstenbinder K, Abel S (2019) The Local Phosphate Deficiency Response Activates Endoplasmic Reticulum Stress-Dependent Autophagy. Plant Physiol. 179(2): 460–476.
Yoshitake Y, Nakamura S, Shinozaki D, Izumi M, Yoshimoto K, Ohta H, Shimojima M (2021) RCB-mediated chlorophagy caused by oversupply of nitrogen suppresses phosphate-starvation stress in plants. ANT PHYSIOLOGY. 185: 318–330
Latchman DS (1997) Transcription factors: An overview. Int J Biochem Cell Biol. 29:1305–1312.
Rubio V, Linhares F, Solano R, Martín AC, Iglesias J, Leyva A, Paz‐Ares J (2001) A conserved MYB transcription factor involved in phosphate starvation signaling both in vascular plants and in unicellular algae. Genes Dev. 15: 2122–2133.
Devaiah BN, Madhuvanthi R, Karthikeyan AS, Raghothama KG (2009) Phosphate starvation responses and gibberellic acid biosynthesis are regulated by the MYB62 transcription factor in Arabidopsis. Mol Plant 2: 43–58.
Chen YF, Li LQ, Xu Q, Kong YH, Wang H, Wu WH (2009b) The WRKY6 transcription factor modulates PHOSPHATE1 expression in response to low Pi stress in Arabidopsis. Plant Cell 21: 3554–3566.
Chen ZH, Nimmo GA, Jenkins GI, Nimmo HG (2007) BHLH32 modulates several biochemical and morphological processes that respond to Pi starvation in Arabidopsis. Biochem J. 405: 191–198.
Fujimoto SY, Ohta M, Usui A, Shinshi H, Ohme-Takagi M (2000) Arabidopsis ethylene-responsive element binding factors act as transcriptional activators or repressors of GCC box–mediated gene expression. Plant Cell. 12: 393–404.
Ohta M, Ohme-Takagi M, Shinshi H (2000). Three ethylene-responsive transcription factors in tobacco with distinct transactivation functions. Plant J. 22: 29–38.
Hiratsu K, Matsui K, Koyama T, Ohme-Takagi M (2003) Dominant repression of target genes by chimeric repressors that include the EAR motif, a repression domain, in Arabidopsis. Plant J. 34: 733–739.
Reece-Hoyes JS and Walhout AJM (2012) YEAST ONE-HYBRID ASSAYS: A HISTORICAL AND TECHNICAL PERSPECTIVE. Methods. 57(4): 441–447.
Reece-Hoyes JS and Walhout AJM (2012) Gene-centered yeast one-hybrid assays. Methods Mol Biol. 812: 189–208.
Mitsuda N, Matsui K, Ikeda M, Nakata M, Oshima Y, Nagatoshi Y, Ohme-Takagi M (2011) CRES-T, An Effective Gene Silencing System Utilizing Chimeric Repressors. Plant Transcription Factors. Methods in Molecular Biology. 754
Harayama T and Riezman H (2018) Understanding the diversity of membrane lipid composition. Nature Reviews Molecular Cell Biology. 19: 281–296.
Poirier Y, Thoma S, Somerville C, Schiefelbein J (1991) Mutant of Arabidopsis deﬁcient in xylem loading of phosphate. Plant Physiol. 97: 1087–1093.
Nakamura Y (2013) Phosphate starvation and membrane lipid remodeling in seed plants. Progress in Lipid Research. 52: 43–50.
Roughan PG, Slack CR (1982) Cellular organization of glycerolipid metabolism. Annu Rev Plant Physiol. 33: 97–132.
Nakamura Y, Awai K, Masuda T, Yoshioka Y, Takamiya K, Ohta H (2005) A novel phosphatidylcholine-hydrolyzing phospholipase C induced by phosphate starvation in Arabidopsis. J Biol Chem. 280: 7469–7476.
Kobayashi K, Awai K, Nakamura M, Nagatani A, Masuda T, Ohta H (2009) Type-B monogalactosyldiacylglycerol synthases are involved in phosphate starvation-induced lipid remodeling, and are crucial for low-phosphate adaptation. Plant J. 57: 322–331.
Yu B, Xu C, Benning C (2002) Arabidopsis disrupted in SQD2 encoding sulfolipid synthase is impaired in phosphate-limited growth. Proc Natl Acad Sci USA. 99: 5732–5737.
Yu L, Zhou C, Fan J, Shanklin J, Xu C (2021) Mechanisms and functions of membrane lipid remodeling in plants. The Plant Journal. 107: 37–53.
Alonso JM, Stepanova AN, Leisse TJ, Kim CJ, Chen H, Shinn P, Stevenson DK, Zimmerman J, Barajas P, Cheuk R, Gadrinab C, Heller C, Jeske A, Koesema E, Meyers CC, Parker H, Prednis L, Ansari Y, Choy N, Deen H, Geralt M, Hazari N, Hom E, Karnes M, Mulholland C, Ndubaku R, Schmidt I, Guzman P, Aguilar-Henonin L, Schmid M, Weigel D, Carter DE, Marchand T, Risseeuw E, Brogden D, Zeko A, Crosby WL, Berry CC, Ecker JR (2003) Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 1. 301(5633): 653-657.
Mitsuda N, Ikeda M, Takada S, Takiguchi Y, Kondou Y, Yoshizumi T, Fujita M, Shinozaki K, Matsui M, Ohme-Takagi M (2010) Efficient Yeast One-/Two-Hybrid Screening Using a Library Composed Only of Transcription Factors in Arabidopsis thaliana. Plant Cell Physiol. 51(12): 2145–2151.
Murashige T, Skoog F (1962) A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures. Physiologia Plantarum. 15(3): 473-497
Gietz RD, Woods RA (2006) Yeast transformation by the LiAc/SS Carrier DNA/PEG method. Methods Mol Biol. 313: 107-120.
Dereeper A, Guignon V, Blanc G, Audic S, Buffet S, Chevenet F, Dufayard JF, Guindon S, Lefort V, Lescot M, Claverie JM, Gascuel O (2008) Phylogeny.fr: robust phylogenetic analysis for the non-specialist. Nucleic Acids Res. 36: 465-9.
Nakamura Y (2013) Phosphate starvation and membrane lipid remodeling in seed plants. Progress in Lipid Research. 52: 43–50.
Yeh C, Kobayashi K, Fujii S, Fukaki H, Mitsuda N, Ohme-Takagi M (2020) Blue Light Regulates Phosphate Deficiency-Dependent Primary Root Growth Inhibition in Arabidopsis. Front. Plant Sci. 10: 1803
Weirauch MT, Yang A, Albu M, Cote AG, Montenegro-Montero A, Drewe P, Najafabadi HS, Lambert SA, Mann I, Cook K, Zheng H, Goity A, Bakel HV, Lozano JC, Galli M, Lewsey MG, Huang E, Mukherjee T, Chen X, Reece-Hoyes JS, Govindarajan S, Shaulsky G, Walhout AJM, Bouget F, Ratsch G, Larrondo LF, Ecker JR, Hughes TR (2014) Determination and inference of eukaryotic transcription factor sequence specificity. Cell. 158(6): 1431-1443.
Chow C, Lee T, Hung Y, Li G, Tseng K, Liu Y, Kuo P, Zheng H, Chang W (2019) "PlantPAN3.0: a new and updated resource for reconstructing transcriptional regulatory networks from ChIP-seq experiments in plants". Nucleic Acids Res. 47: 1155–1163.
Bartlett A, O'Malley RC, Huang SC, Galli M, Nery JR, Gallavotti A, Ecker JR (2017) Mapping genome-wide transcription-factor binding sites using DAP-seq. Nature Protocols. 12: 1659–1672.
Singh A, Kanwa P, Pandey A, Tyagi AK, Sopory SK, Kapoor S, Pandey GK (2013) Comprehensive genomic analysis and expression profiling of phospholipase C gene family during abiotic stresses and development in rice. PLoS One. 30;8(4): e62494.
Schmid M, Davison TS, Henz SR, Pape UJ, Demar M, Vingron M, Schölkopf B, Weigel D, Lohmann JU (2005) A gene expression map of Arabidopsis thaliana development. Nature Genetics. 37:501–506.
Winter D, Vinegar B, Nahal H, Ammar R, Wilson GV, Provart NJ (2007) An “Electronic Fluorescent Pictograph” Browser for Exploring and Analyzing Large-Scale Biological Data Sets. PLoS ONE. 2(8): e718.
Kilian J, Whitehead D, Horak J, Wanke D, Weinl S, Batistic O, D’Angelo C, Bornberg-Bauer E, Kudla J, Harter K (2007) The AtGen Express global stress expression data set: protocols, evaluation and model data analysis of UV-B light, drought and cold stress responses. The Plant Journal. 50: 347-363
Wilkins O, Bräutigam K, Campbell MM (2010) Time of day shapes Arabidopsis drought transcriptomes. The Plant Journal.63
Branco-Price C, Kawaguchi R, Ferreira RB, Bailey-Serres J (2005) Genome-wide Analysis of Transcript Abundance and Translation in Arabidopsis Seedlings Subjected to Oxygen Deprivation.Annals of Botany. 96: 647–660.
Van Hoewyk D, Takahashi H, Inoue E, Hess A, Tamaoki M, Pilon-Smits EAH (2008) Transcriptome analyses give insights into selenium-stress responses and selenium tolerance mechanisms in Arabidopsis. Physiol Plant. 132(2): 236-53.
Secco D, Jabnoune M, Walker H, Shou H, Wu P, Poirier Y, Whelan J (2013) Spatio-temporal transcript profiling of rice roots and shoots in response to phosphate starvation and recovery. Plant Cell. 25(11):4285-4304.
Dereeper A, Guignon V, Blanc G, Audic S, Buffet S, Chevenet F, Dufayard JF, Guindon S, Lefort V, Lescot M, Claverie JM, Gascuel O (2008) Phylogeny.fr: robust phylogenetic analysis for the non-specialist. Nucleic Acids. 36: 465-469.
Rubio V, Linhares F, Solano R, Martin AC, Iglesias J (2001) A conserved MYB transcription factor involved in phosphate starvation signaling both in vascular plants and in unicellular algae. Genes Dev 15: 2122–2133.
Antosch M, Mortensen SA, Grasser KD (2012) Plant Proteins Containing High Mobility Group Box DNA-Binding Domains Modulate Different Nuclear Processes. Plant Physiology. 159: 875–883.
Sánchez-Montesino R, Bouza-Morcillo L, Marquez J, Ghita M, Duran-Nebreda S, Gómez L, Holdsworth MJ, Bassel G, Oñate-Sánchez L (2019) A Regulatory Module Controlling GA-Mediated Endosperm Cell Expansion Is Critical for Seed Germination in Arabidopsis. Molecular Plant 12: 71–85.
Maugarny A, Gonçalves B, Arnaud N, Laufs P (2015) CUC transcription factors: to the meristem and beyond. Plant Transcription Factors. Elsevier. 10: 1016
Wolfram T, Schwarz M, Reuß M, Lossow K, Ost M, Klaus S, Schwerdtle T, Kipp AP (2020) N-Acetylcysteine as Modulator of the Essential Trace Elements Copper and Zinc. Antioxidants (Basel). 9(11): 1117.
Shao H, Wang H, Tang X (2015) NAC transcription factors in plant multiple abiotic stress responses: progress and prospects. Front Plant Sci. 6: 902.
Li W, Li X, Chao J, Zhang Z, Wang W, Guo Y (2018) NAC Family Transcription Factors in Tobacco and Their Potential Role in Regulating Leaf Senescence. Front. Plant Sci. 9:1900.
Puranik S, Sahu PP, Srivastava PS, Prasad M (2012) NAC proteins: Regulation and role in stress tolerance. Trends in Plant Science. 17(6): 369-81.
Wu P, Ma L, Hou X, Wang M, Wu Y, Liu F, Deng X (2003) Phosphate Starvation Triggers Distinct Alterations of Genome Expression in Arabidopsis Roots and Leaves1. Plant Physiol. 132(3): 1260–1271.
Chen G, Snyder CL, Greer MS, Weselake RJ (2011) Biology and biochemistry of plant phospholipases. Crit. Rev. Plant Sci. 30: 239–258.
Fan L, Zheng S, Wang X (1997) Antisense suppression of phospholipase D alpha retards abscisic acid- and ethylene-promoted senescence of postharvest Arabidopsis leaves. Plant Cell. 9: 2183–2196.
Kubo H, Peeters AJ, Aarts MG, Pereira A, Koornneef M (1999) ANTHOCYANINLESS2, a homeobox gene affecting anthocyanin distribution and root development in Arabidopsis. Plant Cell. 11(7): 1217-26
Mabuchi A, Soga K, Wakabayashi K, Hoson T (2016) Phenotypic screening of Arabidopsis T-DNA insertion lines for cell wall mechanical properties revealed ANTHOCYANINLESS2, a cell wall-related gene. J Plant Physiol. 191: 29-35.
Jensen MK, Hagedorn PH, Torres-Zabala MD, Grant MR, Rung JH, Collinge DB, Lyngkjaer MF (2008) Transcriptional regulation by an NAC (NAM–ATAF1,2–CUC2) transcription factor attenuates ABA signalling for efficient basal defence towards Blumeria graminis f. sp. hordei in Arabidopsis. The Plant Journal. 56: 867–880.
Wu Y, Deng Z, Lai J, Zhang Y, Yang C, Yin B, Zhao Q, Zhang L, Li Y, Yang C, Xie Q (2009) Dual function of Arabidopsis ATAF1 in abiotic and biotic stress responses. Cell Research. 19: 1279-1290.
Jensen MK, Lindemose S, deMasi F, Reimer JJ, Nielsen M, Perera V, Workman CT, Turck F, Grant MR, Mundy J, Petersen M, Skrivera K (2013) ATAF1 transcription factor directly regulates abscisic acid biosynthetic gene NCED3 in Arabidopsis thaliana. FEBS Open Bio. 3: 321-327
Jiang W, Wu J, Zhang Y, Yin L, Lu J (2015) Isolation of a WRKY30 gene from Muscadinia rotundifolia (Michx) and validation of its function under biotic and abiotic stresses. Protoplasma. 252: 1361–1374.
Guo Z, Fujioka S, Blancaflor EB, Miao S, Gou X, Li J (2010) TCP1 modulates brassinosteroid biosynthesis by regulating the expression of the key biosynthetic gene DWARF4 in Arabidopsis thaliana. Plant Cell. 22(4): 1161-1173.
Chung M, Lee S, Min J, Huang P, Ju H, Kim C (2016) Regulation of Arabidopsis thaliana plasma membrane glucose-responsive regulator (AtPGR) expression by A. thaliana storekeeper-like transcription factor, AtSTKL, modulates glucose response in Arabidopsis. Plant Physiol Biochem. 104: 155-164.
Li S and Zachgo S (2013) TCP3 interacts with R2R3-MYB proteins, promotes flavonoidbiosynthesis and negatively regulates the auxin response inArabidopsis thalianaShutian. The Plant Journal. 76: 901–913.
Rodríguez MC, Wawrzynska A, Sirko A (2014) Intronic T-DNA insertion in Arabidopsis NBR1conditionally affects wild-type transcript level. Plant Signaling & Behavior 9:12

2026-03-01公開

簡易檢索 / 詳目顯示

相關論文