環境荷爾蒙為干擾人體內分泌系統運作的物質，常用於各種工業產品，其對於環境與健康的潛在危害一直被人們所重視。其中雙酚A是最常見的環境荷爾蒙之一，已被用來製作出許多生活用品。在去年我國發生毒奶瓶事件，許多來自大廠的奶瓶被偵測出超量的雙酚A，自此之後雙酚A之危害再度人們被重視。

本論文以一系列電化學製程製作出金奈米薄膜已達到表面增強拉曼效果，並以三聚氰胺對金奈米薄膜進行表面改質後，能以拉曼增強來偵測水中微量的雙酚A，利用電化學方法和拉曼增強偵測的優點，達到快速且有效的偵測，其檢測限可達10-7-10-8M。

In this work, a facile electrochemical procedure has been proposed for the fabrication of melamine-functionalized gold nanoparticles on ITO electrode as SERS substrates for detection of bisphenol A. First, seed-mediated growth was used to deposit gold nanoparticles. With the growth of the nanoparticles, the gap between the adjacent nanoparticles gradually decreases, and thus plenty of “hot spots” are formed on the substrate for SERS applications. Second, melamine functionalization on SERS substrates was based on diazotization, which is an electro-initiate process.The procedure for melamine-functionalized SERS substrates was cheap, rapid, and eco-friendly with full control by electrochemical procedure. The substrate was used to detect bisphenol A, a typical endocrine disruptor in the environment to show its sensitivity and stability.

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