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研究生： 蕭立文
Hsiao, Li-Wen
論文名稱： 利用乳化反應製備交聯共聚高分子奈米膠
Synthesis of Nanogels Based on Cross-linked Copolymers Using Emulsion
指導教授： 詹正雄
Jan, Jeng-Shiung
學位類別： 碩士
Master
系所名稱： 工學院 - 化學工程學系
Department of Chemical Engineering
論文出版年： 2016
畢業學年度： 104
語文別： 中文
論文頁數： 150
中文關鍵詞： 奈米膠 、聚胺基酸 、乳化反應 、交聯反應
外文關鍵詞： nanogels, polypeptide, emulsion, cross-link
相關次數： 點閱：80  下載：2 
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    • 本研究成功合成均聚賴胺酸(homo-poly(L-lysine)，PLL)、聚乙二醇與聚賴胺酸嵌段共聚物(PEG-b-poly(L-lysine)，PEG-b-PLL)、聚賴胺酸與聚蘇胺酸嵌段共聚物(poly(L-lysine)-b-poly(L-threonine)，PLL-b-PLT)以及聚賴胺酸與聚肌胺酸嵌段共聚物(poly(L-lysine)-b-poly(sarcosine)，PLL-b-PSar)。將聚胺酸藉由油中液滴型乳化反應(water-in-oil emulsion)與京尼平(genipin)交聯鍵結，製備生物相容性高的奈米膠體粒子。聚胺酸奈米膠體粒子具有穩定立體網狀結構，相較於傳統載體可提升藥物/蛋白質的裝載比例。透過調控聚胺基酸種類、嵌段共聚比例、交聯程度、聚胺基酸重量百分比濃度以及裝載的蛋白質含量等，設計不同性質的奈米膠體。本研究製備包覆肌紅蛋白(Myoglobin)的奈米膠載體，以1H NMR、MALDI-TOF、GPC、FT-IR、CD、DLS、Zeta Potential、MTT與UV/vis spectrum分別進行聚胺基酸與奈米膠體粒子的分析鑑定。由實驗結果可知PLL-b-PSar系列的奈米膠粒徑最小(119.6 ±1.9 nm)且粒徑分布較其他聚胺酸系列製備的奈米膠窄(0.32 ±0.02)，經由細胞毒性測試證實其生物相容性高。裝載肌紅蛋白的奈米膠PLL33-b-PSar50-0.43Mb粒徑為197.7 ±8.9 nm，PDI則為0.25 ±0.02，並使用UV/vis 確認肌紅蛋白仍具有生物功能，表示利用乳化反應製備交聯共聚高分子奈米膠深具做為蛋白質載體的潛力。

    We report the synthesis of biocompatible nanogels based on cross-linked, double hydrophilic block copolymer by using emulsion. Nanogels, which are three-dimensional polymeric networks, can provide advantages for delivery system because that the interior structure accommodate biomolecules like drug, protein, or enzyme. Nanogels with different functionalities can be designed through varying polymer composition, block ratio, and cross-linking degree. These nanogels were used to encapsulate proteins and the encapsulated proteins can still retain its bioactivities. The results suggested that these as-prepared nanogels would have potential applications in areas such as drug carriers and encapsulants.

    摘要 i Extended Abstract ii 誌謝 xii 目錄 xiv 表目錄 xviii 圖目錄 xx 第一章 緒論 1 1.1 前言 1 1.2 研究動機 3 1.3 研究構想 3 第二章 文獻回顧 5 2.1 聚胺基酸 5 2.1.1 胺基酸的基本性質 5 2.1.2 胺基酸與蛋白質之結構 8 2.1.3 胺基酸之聚合反應 10 2.2 奈米膠 14 2.2.1 奈米膠之定義 14 2.2.2 奈米膠之種類 15 2.3 交聯反應 18 2.4 奈米膠之應用 22 2.4.1 藥物/蛋白質載體 23 2.4.2 肌紅蛋白之特性 24 第三章 實驗方法與步驟 26 3.1 實驗藥品 26 3.2 聚胺基酸合成 29 3.2.1 開環聚合法N-carboxylanhydrides (NCAs) 29 3.2.2 乾燥溶劑 29 3.2.3 Z-L-lysine NCAs製備 30 3.2.4 N-Boc-O-benzyl-L-threonine NCAs之製備 31 3.2.5 Sarcosine NCAs之製備 33 3.2.6 利用不同起始劑對NCAs開環合成聚胺基酸 36 3.2.7 去除聚胺基酸的保護基 39 3.3 奈米膠之製備 41 3.3.1 奈米膠之乳化製程 41 3.3.2 奈米膠之實驗參數 42 3.4 奈米膠之應用 44 3.4.1 奈米膠裝載肌紅蛋白(Myoglobin) 44 3.4.2 奈米膠裝載肌紅蛋白之實驗參數 45 3.5 實驗儀器與原理 47 3.5.1 液態核磁共振儀(1H NMR) 47 3.5.2 基質輔助雷射脫附游離飛行時間質譜儀(MALDI-TOF) 48 3.5.3 凝膠滲透層析儀(GPC) 49 3.5.4 傅立葉轉換紅外線光譜儀(FT-IR) 50 3.5.5 圓二色光譜儀(CD) 52 3.5.6 動態光散射儀(DLS) 54 3.5.7 介達電位分析儀 55 3.5.8 穿透式電子顯微鏡(TEM) 56 3.5.9 紫外光/可見光光譜儀(UV/vis) 57 3.6 聚胺基酸與奈米膠之性質測試參數 58 3.6.1 液態核磁共振儀(1H NMR) 58 3.6.2 基質輔助雷射脫附游離飛行時間質譜儀(MALDI-TOF) 58 3.6.3 凝膠滲透層析儀(GPC) 59 3.6.4 傅立葉轉換紅外線光譜儀(FT-IR) 59 3.6.5 圓二色光譜儀(CD) 59 3.6.6 動態光散射儀(DLS) 60 3.6.7 穿透式電子顯微鏡(TEM) 60 3.6.8 穩定性測試 60 3.6.9 細胞毒性測試 61 第四章 結果與討論 63 4.1 聚胺基酸之分析 63 4.1.1 聚胺基酸之聚合度 64 4.1.2 聚胺基酸之分子量 75 4.1.3 聚胺基酸之二級結構 82 4.2 奈米膠之分析 87 4.2.1 奈米膠之形成 87 4.2.2 奈米膠之粒徑 90 4.2.3 奈米膠之穩定性 106 4.2.4 奈米膠之二級結構 110 4.2.5 奈米膠之介達電位 120 4.2.6 奈米膠之細胞毒性測試 126 4.3 奈米膠肌紅蛋白載體之分析 128 4.3.1 奈米膠肌紅蛋白載體之形成 128 4.3.2 奈米膠肌紅蛋白載體之粒徑 129 4.3.3 奈米膠肌紅蛋白載體之包覆率 131 4.3.4 奈米膠肌紅蛋白載體之生物功能 133 第五章 結論 136 第六章 參考文獻 139

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