由於微流體具有低成本、檢測的反應量少、且反應快速等優勢，因此隨著科技進步微流體技術越來越受到研究學者關注。除此之外，人工纖毛被視為一種有效操縱流場的裝置，然而結合人工纖毛與微流體技術將大幅提升微流體系統的效能。本論文提出了兩種利用磁性人工纖毛微流體裝置的應用方式，分別為透過磁場控制微流體裝置內人工纖毛以提升生物細胞之活性和智慧型材料之光降解率。其中本研究中的生物細胞為斑馬魚精子，為了活化斑馬魚精子細胞，本實驗在人工纖毛微流道中以主動式混合，透過磁驅動系統控制流道中之人工纖毛，藉以產生渦流及環流的流場結構；此外，透過計算精子細胞移動距離並進行影像處理分析精子細胞活性，可以得到最高之活化率74.44 ％ ± 6.07，證明出本實驗在磁性人工纖毛轉動對於斑馬魚精子細胞之活化率有極大之效益。除此之外，本論文結合人工纖毛微流體裝置於光觸媒材料，為了可以使光觸媒材料在短時間內可以使環境產生光降解反應，本實驗透過微控制器驅動人工纖毛，藉以改變流場渦流結構；此外，透過微粒子影像測速儀進行流場分析，證明出有人工纖毛轉動可以有效產生光催化反應，在60分鐘的光降解率可以達到81.7%，說明人工纖毛轉動對於提高光催化效率是有效的。本論文所提出的人工纖毛微流體裝置除了能幫助生醫產業之生物育種，亦可當作感應器並結合多項功能於單一的裝置當中。

The microfluidic technology recently has gained attention from worldwide researchers due to scientific and technological advantages compared to conventional experimental techniques. The artificial cilia based microfluidic device is a latest feat in the microfluidic research that provides unprecedented advantage in flow manipulation in microscale. This thesis proposes two artificial cilia based microfluidic device to handle two tasks such as biological species (zebrafish sperm specimen) and photodegradation process. In the first task, a serpentine microfluidics was proposed for efficient micromixing towards rapid, reliable cryopreserved sperm activation where 74.44 ± 6.07 % of the used sperm were activated. A detailed hydrodynamic analysis suggests that artificial cilia instigates a vortex and circulation near the wall and center of the microchannel enhancing the micromixing so do the sperm activation. In the second task, it is shown that with the combination of SnFe2O4 nanoparticles and magnetic artificial cilia, a highly efficient catalytic activity can be achieved under the visible light through a better mixing performance. To identify the optimal advanced oxidation process using the selected photocatalyst running with the microfluidics, a micro-particle image velocimetry (µPIV) analysis was carried out for three different modes of artificial cilia actuation. A superior performance was achieved with a maximum degradation rate of 81.7 % in 60 minutes using the presented design with the cilia actuating in a circular motion. The future application of these devices will be focused towards real time sensing of its environment and to perform multi-tasking in a microfluidic environment.

[1] L. T. Haimo and J. L. Rosenbaum, "Cilia, flagella, and microtubules," J Cell Biol, vol. 91, pp. 125s-130s, 1981.
[2] P. Satir and S. T. Christensen, "Overview of structure and function of mammalian cilia," Annu. Rev. Physiol., vol. 69, pp. 377-400, 2007.
[3] N. Spassky and A. Meunier, "The development and functions of multiciliated epithelia," Nature reviews Molecular cell biology, vol. 18, p. 423, 2017.
[4] J. C. Ho, K. N. Chan, W. H. Hu, W. K. Lam, et al., "The effect of aging on nasal mucociliary clearance, beat frequency, and ultrastructure of respiratory cilia," American journal of respiratory and critical care medicine, vol. 163, pp. 983-988, 2001.
[5] W. Gilpin, V. N. Prakash, and M. Prakash, "Vortex arrays and ciliary tangles underlie the feeding–swimming trade-off in starfish larvae," Nature Physics, vol. 13, p. 380, 2017.
[6] S. Khaderi, J. den Toonder, and P. Onck, "Microfluidic propulsion by the metachronal beating of magnetic artificial cilia: a numerical analysis," Journal of Fluid Mechanics, vol. 688, pp. 44-65, 2011.
[7] D. Smith, J. Blake, and E. Gaffney, "Fluid mechanics of nodal flow due to embryonic primary cilia," Journal of The Royal Society Interface, vol. 5, pp. 567-573, 2008.
[8] M. Downton and H. Stark, "Beating kinematics of magnetically actuated cilia," EPL (Europhysics Letters), vol. 85, p. 44002, 2009.
[9] Y.-A. Wu, B. Panigrahi, Y.-H. Lu, and C.-Y. Chen, "An Integrated Artificial Cilia Based Microfluidic Device for Micropumping and Micromixing Applications," Micromachines, vol. 8, p. 260, 2017.
[10] B. Evans, A. Shields, R. L. Carroll, S. Washburn, et al., "Magnetically actuated nanorod arrays as biomimetic cilia," Nano letters, vol. 7, pp. 1428-1434, 2007.
[11] A. Shields, B. Fiser, B. Evans, M. Falvo, et al., "Biomimetic cilia arrays generate simultaneous pumping and mixing regimes," Proceedings of the National Academy of Sciences, vol. 107, pp. 15670-15675, 2010.
[12] J. den Toonder, F. Bos, D. Broer, L. Filippini, et al., "Artificial cilia for active micro-fluidic mixing," Lab on a Chip, vol. 8, pp. 533-541, 2008.
[13] C. L. Van Oosten, C. W. Bastiaansen, and D. J. Broer, "Printed artificial cilia from liquid-crystal network actuators modularly driven by light," Nature materials, vol. 8, p. 677, 2009.
[14] J. Belardi, N. Schorr, O. Prucker, and J. Rühe, "Artificial cilia: generation of magnetic actuators in microfluidic systems," Advanced Functional Materials, vol. 21, pp. 3314-3320, 2011.
[15] M. Vilfan, A. Potočnik, B. Kavčič, N. Osterman, et al., "Self-assembled artificial cilia," Proceedings of the National Academy of Sciences, vol. 107, pp. 1844-1847, 2010.
[16] J. Hussong, N. Schorr, J. Belardi, O. Prucker, et al., "Experimental investigation of the flow induced by artificial cilia," Lab on a Chip, vol. 11, pp. 2017-2022, 2011.
[17] Y. Wang, Y. Gao, H. M. Wyss, P. D. Anderson, et al., "Artificial cilia fabricated using magnetic fiber drawing generate substantial fluid flow," Microfluidics and Nanofluidics, vol. 18, pp. 167-174, 2015.
[18] C.-C. Hong, J.-W. Choi, and C. H. Ahn, "A novel in-plane passive microfluidic mixer with modified Tesla structures," Lab on a Chip, vol. 4, pp. 109-113, 2004.
[19] K. Oh, B. Smith, S. Devasia, J. J. Riley, et al., "Characterization of mixing performance for bio-mimetic silicone cilia," Microfluidics and nanofluidics, vol. 9, pp. 645-655, 2010.
[20] M. Rahbar, L. Shannon, and B. L. Gray, "Microfluidic active mixers employing ultra-high aspect-ratio rare-earth magnetic nano-composite polymer artificial cilia," Journal of Micromechanics and Microengineering, vol. 24, p. 025003, 2013.
[21] W. Wang, Z. Yao, J. C. Chen, and J. Fang, "Composite elastic magnet films with hard magnetic feature," Journal of Micromechanics and Microengineering, vol. 14, p. 1321, 2004.
[22] B. Panigrahi, C.-H. Lu, N. Ghayal, and C.-Y. Chen, "Sperm activation through orbital and self-axis revolutions using an artificial cilia embedded serpentine microfluidic platform," Scientific reports, vol. 8, p. 4605, 2018.
[23] C. Pardo-Martin, T.-Y. Chang, B. K. Koo, C. L. Gilleland, et al., "High-throughput in vivo vertebrate screening," Nature methods, vol. 7, p. 634, 2010.
[24] M. H. Malone, N. Sciaky, L. Stalheim, K. M. Hahn, et al., "Laser-scanning velocimetry: a confocal microscopy method for quantitative measurement of cardiovascular performance in zebrafish embryos and larvae," BMC biotechnology, vol. 7, p. 40, 2007.
[25] K. Howe, M. D. Clark, C. F. Torroja, J. Torrance, et al., "The zebrafish reference genome sequence and its relationship to the human genome," Nature, vol. 496, p. 498, 2013.
[26] C. Chakraborty, A. R. Sharma, G. Sharma, and S.-S. Lee, "Zebrafish: A complete animal model to enumerate the nanoparticle toxicity," Journal of nanobiotechnology, vol. 14, p. 65, 2016.
[27] M. Morisawa, K. Suzuki, and S. Morisawa, "Effects of potassium and osmolality on spermatozoan motility of salmonid fishes," Journal of Experimental Biology, vol. 107, pp. 105-113, 1983.
[28] S. Oda and M. Morisawa, "Rises of intracellular Ca2+ and pH mediate the initiation of sperm motility by hyperosmolality in marine teleosts," Cytoskeleton, vol. 25, pp. 171-178, 1993.
[29] H. Takai and M. Morisawa, "Change in intracellular K+ concentration caused by external osmolality change regulates sperm motility of marine and freshwater teleosts," Journal of Cell Science, vol. 108, pp. 1175-1181, 1995.
[30] J. Cosson, "The ionic and osmotic factors controlling motility of fish spermatozoa," Aquaculture International, vol. 12, pp. 69-85, 2004.
[31] L. Chauvaud, J. Cosson, M. Suquet, and R. Billard, "Sperm motility in turbot, Scophthalmus marimus: initiation of movement and changes with time of swimming characteristics," Environmental Biology of Fishes, vol. 43, pp. 341-349, 1995.
[32] T. Scherr, G. L. Knapp, A. Guitreau, D. S.-W. Park, et al., "Microfluidics and numerical simulation as methods for standardization of zebrafish sperm cell activation," Biomedical microdevices, vol. 17, p. 65, 2015.
[33] M. Elsayed, T. M. El-Sherry, and M. Abdelgawad, "Development of computer-assisted sperm analysis plugin for analyzing sperm motion in microfluidic environments using Image-J," Theriogenology, vol. 84, pp. 1367-1377, 2015.
[34] J. G. Wilson-Leedy and R. L. Ingermann, "Development of a novel CASA system based on open source software for characterization of zebrafish sperm motility parameters," Theriogenology, vol. 67, pp. 661-672, 2007.
[35] T. M. El-Sherry, M. Elsayed, H. K. Abdelhafez, and M. Abdelgawad, "Characterization of rheotaxis of bull sperm using microfluidics," Integrative Biology, vol. 6, pp. 1111-1121, 2014.
[36] K.-T. Lee and S.-Y. Lu, "A cost-effective, stable, magnetically recyclable photocatalyst of ultra-high organic pollutant degradation efficiency: SnFe 2 O 4 nanocrystals from a carrier solvent assisted interfacial reaction process," Journal of Materials Chemistry A, vol. 3, pp. 12259-12267, 2015.
[37] H. W. Laale, "The biology and use of zebrafish, Brachydanio rerio in fisheries research," Journal of Fish Biology, vol. 10, pp. 121-173, 1977.
[38] D. S. Park, R. A. Egnatchik, H. Bordelon, T. R. Tiersch, et al., "Microfluidic mixing for sperm activation and motility analysis of pearl Danio zebrafish," Theriogenology, vol. 78, pp. 334-344, 2012.
[39] J. S. Wolenski and N. H. Hart, "Scanning electron microscope studies of sperm incorporation into the zebrafish (Brachydanio) egg," Journal of Experimental Zoology Part A: Ecological Genetics and Physiology, vol. 243, pp. 259-273, 1987.
[40] C.-Y. Chen, C.-Y. Chen, C.-Y. Lin, and Y.-T. Hu, "Magnetically actuated artificial cilia for optimum mixing performance in microfluidics," Lab on a Chip, vol. 13, pp. 2834-2839, 2013.
[41] T.-H. Kim, S. D. Kim, H. Y. Kim, S. J. Lim, et al., "Degradation and toxicity assessment of sulfamethoxazole and chlortetracycline using electron beam, ozone and UV," Journal of hazardous materials, vol. 227, pp. 237-242, 2012.
[42] C. Hsueh, Y. Huang, C. Wang, and C.-Y. Chen, "Degradation of azo dyes using low iron concentration of Fenton and Fenton-like system," Chemosphere, vol. 58, pp. 1409-1414, 2005.
[43] J. Wu, W. Pu, C. Yang, M. Zhang, et al., "Removal of benzotriazole by heterogeneous photoelectro-Fenton like process using ZnFe2O4 nanoparticles as catalyst," Journal of Environmental Sciences, vol. 25, pp. 801-807, 2013.
[44] L. Xu and J. Wang, "Magnetic nanoscaled Fe3O4/CeO2 composite as an efficient Fenton-like heterogeneous catalyst for degradation of 4-chlorophenol," Environmental science & technology, vol. 46, pp. 10145-10153, 2012.
[45] H. Elmoussaoui, M. Hamedoun, O. Mounkachi, A. Benyoussef, et al., "New results on Magnetic Properties of Tin-Ferrite Nanoparticles," Journal of superconductivity and novel magnetism, vol. 25, pp. 1995-2002, 2012.
[46] S. Huang, Y. Xu, M. Xie, H. Xu, et al., "Synthesis of magnetic CoFe2O4/g-C3N4 composite and its enhancement of photocatalytic ability under visible-light," Colloids and Surfaces A: Physicochemical and Engineering Aspects, vol. 478, pp. 71-80, 2015.
[47] T.-H. Yu, W.-Y. Cheng, K.-J. Chao, and S.-Y. Lu, "ZnFe 2 O 4 decorated CdS nanorods as a highly efficient, visible light responsive, photochemically stable, magnetically recyclable photocatalyst for hydrogen generation," Nanoscale, vol. 5, pp. 7356-7360, 2013.
[48] Y. Liu, L. Yu, Y. Hu, C. Guo, et al., "A magnetically separable photocatalyst based on nest-like γ-Fe 2 O 3/ZnO double-shelled hollow structures with enhanced photocatalytic activity," Nanoscale, vol. 4, pp. 183-187, 2012.
[49] M. Krivec, K. Žagar, L. Suhadolnik, M. Čeh, et al., "Highly efficient TiO2-based microreactor for photocatalytic applications," ACS applied materials & interfaces, vol. 5, pp. 9088-9094, 2013.
[50] G. M. Whitesides, "The origins and the future of microfluidics," Nature, vol. 442, p. 368, 2006.
[51] R. Gorges, S. Meyer, and G. Kreisel, "Photocatalysis in microreactors," Journal of Photochemistry and Photobiology A: Chemistry, vol. 167, pp. 95-99, 2004.
[52] K. Jähnisch, V. Hessel, H. Löwe, and M. Baerns, "Chemistry in microstructured reactors," Angewandte Chemie International Edition, vol. 43, pp. 406-446, 2004.
[53] J. Parmar, S. Jang, L. Soler, D.-P. Kim, et al., "Nano-photocatalysts in microfluidics, energy conversion and environmental applications," Lab on a Chip, vol. 15, pp. 2352-2356, 2015.