炭疽病是一種會導致經濟作物重大的經濟損失的真菌疾病，其中最常見引起大豆炭疽病的病原體是C. truncatum。目前關於抗炭疽病的大豆品種研究信息很少。本研究的目的是希望從亞洲蔬菜中心和台灣本土開發的不同種質和優良育種系中篩選出對C. truncatum有抗性的品種。在三次的接種試驗中，高雄9號作為敏感對照組(SC)，Mandarin 507為抗性對照組(RC)。在第二和第三次的接種試驗中有五個基因型（VI017590、VI063762、VI027011、VI026746 和 VI014587）對 C. truncatum 具有抗性，但這五個品種並沒有對炭疽病完全免疫。找出五個具有抗性的大豆品系後，希望開發與抗炭疽病相關的SSR標記，但是分析上發現這些品系的遺傳訊息不穩定，造成判斷上的困難，因此需要更多的前置研究，例如發展純合子或純係的大豆品種。於是利用組織培養高雄9號和Mandarin 507，希望可以先培養出純合子。在高雄9號和Mandarin 507中可透過未成熟大豆的胚軸誘導出癒傷組織並生成芽或根，但是發展到成熟的大豆植株還需要更多的培養基測試。

Anthracnose is a fungal disease that can cause significant economic losses to cash crops. The most common pathogen causing soybean anthracnose is C. truncatum. There is little research information on soybean varieties resistant to anthracnose. The purpose of this study is to screen out varieties that are resistant to C. truncatum from different germplasm and excellent breeding lines developed by the WorldVeg and Taiwan. Kaohsiung No. 9 was used as the sensitive control (SC) in the three inoculation trials, and Mandarin 507 was used as the resistance control (RC). In the second and third inoculation trials, five genotypes (VI017590, VI063762, VI027011, VI026746, and VI014587) were resistant to C. truncatum. However, five varieties were not fully immune to anthracnose. After identifying five resistant soybean lines, I hope to develop SSR markers related to anthracnose resistance. However, the genetic information of these lines is found to be unstable in analysis, and more pre-research is needed, such as the development of the homozygote or pure soybean varieties. Therefore, we used the tissue culture of Kaohsiung No.9 and Mandarin 507, hoping to cultivate homozygotes first. In Kaohsiung 9 and Mandarin 507, callus can be induced through the embryo axis of immature soybeans and form shoots or roots; however, more medium testing is needed to develop mature soybean plants.

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