傳統雜交實驗中，靜置與微擾動之樣本藉由擴散機制，補充與探針結合所消耗之樣本，此反應通常需要16~18小時，對於進行大量、快速樣本檢測，實為一大阻礙。因此本論文提出一能提昇雜交效率之方法，藉由雙向微流驅動裝置，使填滿於反應區之樣本，能於反應過程中往復流動，使樣本以對流機制，補充與探針結合所消耗之樣本，且所設計之反應區，使往復流動樣本於載玻片點印探針區域，具有較大之伸展應變率，可使得樣品中之DNA拉長，且增加DNA與探針之碰撞機率，因而提高雜交效率。
微流體雜交晶片包含雙向微流驅動裝置、反應區、點印探針之載玻片與溫度控制裝置。雙向微流驅動裝置包含吸、推元件，當空氣注入吸元件時，能於反應區產生吸力；當空氣注入推元件時，能於反應區產生推力。以LabVIEW程式控制電磁閥，將幫浦輸出之空氣於吸、推元件間作快速切換，便能將填滿於反應區之流體產生往復流動之效果，系統之最大震盪頻率為2.25Hz。反應區採翻模方式製作，為PDMS材質，可確保於其中之樣品不易滲漏。溫度控制裝置，能使晶片保持在實驗條件溫度。論文中進行傳統雜交實驗與微流體雜交實驗，並加以比較。由實驗結果可得知，所設計之晶片能提高雜交效率。

The conventional hybridization method often takes 16 to 18 hours to replenish target, which is hybridized with the probe by diffusion, and impediment for large number and fast examining. The present study brings up a method to improve hybridization efficiency by a bi-directional device, which drives samples in the reaction area to flow back and forth, to replenish target, which is hybridized with probe by convection. The designed reaction area, at which the position of probe having higher extensional strain rate by back and forth target mixture flow. DNA can be stretched at this higher extensional strain rate area, and the base pair of DNA can be exposed to complementary target. Microfluidic hybridization chip is consist of a bi-directional driving device, a reaction area, spotted slide and temperature controlled device. The system can control solenoid valves, which is connected to suction and exclusion component, to fast repeat on and off to drive the sample solution in the reaction area flowing back and forth by LabVIEW controlled software. The maxmium oscillation frequency of the system is 2.25 Hz.
Hybridization by conventional and microfluidic method was presented and compared in this work. Results reveal that the designed microflluidic hybridization chip can improve hybridization efficiency.

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