柳橙是台灣產量大且產期集中的水果之一，盛產期往往供過於求，若再加上外銷不通暢時，導致價格慘跌，影響農民生計甚劇。除果肉可食用、果汁可加工外，每年丟棄的果皮數量亦相當可觀，如可將其收集並萃取其有效成分運用於醫療或美容機能性產品上，不僅可以使廢棄物減量且可資源再利用，亦可為台灣柑橘產業開創新的契機。
本研究主要針對提升產品附加價值為主要目標，將柳橙中之有效活性成份:精油、橙皮苷進行各種有效之萃取及分離實驗。精油部分以二氧化碳超臨界萃取，其條件為溫度40℃，壓力4000psi，靜置時間2小時可得最佳產率。橙皮苷在改變各種實驗條件下得到最佳萃取方式後，進行不同月份柳橙之橙皮苷含量分析。

另外本研究亦將具美容潛力之橙皮苷與芸香素，進行抗黑色素之美白實驗，來對照測試其美白效果。其中橙皮苷可延長維生素C抗黑色素之效果。

Citrus is one of the seasonal fruits being mass produced in Taiwan. Its over supply often leads to a significant price drop and consequently impacts on the livelihood of the hardworking farmers. Citrus offers a variety of applications; besides the flesh and juice for dietary, the peel can be extracted for medical or cosmetic uses. This approach not only turns waste into valuables, but also opens a new era for the Taiwanese Citrus industry.
This research is focused on enhancing the value of the unripe Citrus. Experiments have been carried out to extract essential oils and Hesperidin from the citrus. For essential oil , CO2 Supercritical Fluid Extraction technique at 40℃ /4000psi. and 2 hours were employed to achieve complete extraction and maximum yield. Once the optimum conditions are determined, the Hesperidin content was analyzed on citrus that is harvested at different seansons.

In addition, skin whitening studies have been tested on Hesperidin and Rutin. It was found that Hesperidin has the effect on prolonging Vitamin C’s anti-oxidation ability to suppress melanin.

1. 范念慈，果樹，東大圖書公司. 2001.
2. 施明男、陳國雄等，台灣青果名錄，農林廳種苗改良場. 1982.
3. 行政院農業委員會，96年農業統計年報. 2007
4. 沈兆敏，中國柑橘區劃與柑橘良種，中國農業科技出版社. 1986.
5. 李時珍(明)，本草綱目，文化圖書公司. 1994.
6. Yildizogle-Ari N., Altan V. M. et al, Pharmacological effects of rutin. Phytotherapy Tes 5:19-23. 1991.
7. Jenkins K. J., Hidiroglou M. and Collins F. W., Influence of various flavonoids and simple phenolics on development of excudative diathesis in the chick. J. Agric Food Chem. 41:441-445. 1993.
8. Chiba H. , Uehara M. et al, Hesperidin, a citrus flavonoid, inhibits bone loss and decreases serum and hepatic lipids in ovariectomized mice., Journal of Nutrition . 133(6):1892-7. 2003.
9. Buckshee K., Takkar D. and Aggarwal N., Micronized flavonoid therapy in internal hemorrhoids of pregnancy. Int J Gynaecol Obstet. 57(2): 145-151. 1997.
10. Forkmann G., Flavonoids as flower pigments: the formation of natural spectrum and its extension by genetic engineering. Plant Breed 106:1-26. 1991.
11. Griffith J. Q., Couch J. F. and Lindauer M. A., Effect of rutin on increased capillary fragility in man. Proc. Soc. Exptl. Biol. Med. 55: 228-229. 1944.
12. Iwata K., Miwa S., et al., Effects of kangra budkwheat on spontaneously hypertensive rats. J. Kagawa Nutrition College . 21:55-61. 1990.
13. 羅珮文，台灣數種特有水果抗氧化活性及清除自由基能力之評估, 輔仁大學食品營養學系. 2000.
14. 陳彥宏，柑橘類植物果皮之超氧化物歧化SOD活性之研究，國立台灣大學化學研究所. 1997.
15. Cook N. C. and Samman S., Flavonoids-chemistry, metabolism, cardioprotective effects, and dietary sources. Nutr. Biochem. 7:66-67 1996.
16. Winston J. C., Phytochemicals : guardians of our health , J. Am. Diet Assoc. 19: 199-204. 1997
17. Rajnarayana K., Redy M. S. et al, Bioblavonoids classification pharmacological effects and therapeutic potential. Inidia J. Pharmaco. 33: 2-16. 2001.
18. Deschnener E. E., The effect of dietary quercetin and rutin on AOM-induce acute colonic epithelial abnormalities in mice fed a high-fat diet. Nutr. Cancer. 20: 199-204. 1993.
19. Kawaii S., Tomono Y. et al., Differentiating activity and flavonoid content of the readily extractable fraction prepared form Citrus juices. J. Agric. Food Chem. 47: 128-135. 1999.
20. Marchand L., Murphy S. P. and Wilkens L. K., Intake of flavonoids and lung cancer. J. natl. cancer. 92: 154-160. 2000.
21. Galati E. M., Monforte M. T. et al., Biological effects of hesperidin, a citrus flavonoid. (note I): antiinflamatory and analgesic activity. II Farmaco 49: 709-712. 1994.
22. Struckmann M. D. and Nicolaides M. S., A view of pharmacology and therapeutic effect of Daflon 500mg in patients with chronic venous insufficiency and related disorders. J. Vasc. Dis. 45: 419-428. 1994.
23. Monforte M. T., Trovato et al., Biological effects of hesperidin,, a Citrus flavonoid.(note II): Hypolipidemic activity on experimental hypercholesterolemia in rat. II Farmaco 50: 595-599. 1995.
24. Hertog M., Epidemiological evidence on potential health properties of flavonoids. Proc. Nutr. Soc. 55: 385-397. 1996.
25. Heim K. E., Tagliaferro A.R. and Bobilya D. J., Flavonoid antioxidants : chemistry , metabolism and structure-activity relationships. J. Nutr. Biochem. 13: 572-584. 2002.
26. John A. M. and Karel G., Concentrations of hesperidin and other orange pee flavonoids in citrus processing byproducts. J. Agric. Food Chem. 44: 811-814. 1999.
27. 李輝 ，類黃酮和類化合物對食物致突變物之抗突變性與其化學構造間之相關性及抗突變機轉之研究, 國立中興大學食品科技研究所.　1990.
28. 蔡慧玲 ，抗壞血酸與多酚化合物抗氧化性及促氧化性之研究，國立中興大學食品科學系. 1998.
29. 陳志雄 ，黃色素類及類黃酮類中藥單離成份在抑制血管收縮素轉換酵素上之研究分析，國立台灣大學生化科學研究所. 1990.
30. 蔡旻都 ，蔬果中類黃酮之抗氧化作用與生物活性，國立中正大學化學所. 2005.
31. 張正怡 ，探討類黃酮類成分對人類皮膚纖維母細胞生成膠原蛋白之作用，臺北醫學大學藥學研究所. 2006.
32. 盧春秀 ，紅豆及葡萄皮醇溶粗萃物對抑制酪胺酸酶活性之探討，中興大學食品暨應用生物科技學系. 2006.
33. 黃凱聲 ，類黃酮與維他命C反應活性之比較，東海大學化學系. 2008
34. 王超賢 ，Hesperidin 及 hesperidin-3’-O-methylether 抗氣喘的作用，臺北醫學大學醫學科學研究所. 2007.
35. 周均澤，台灣柑橘類植物花部與皮部成份研究，國立台灣大學化學研究所碩士論文. 1980.
36. 長谷川博，最新之柑橘香味料，Beverage Japan,(165):36-38. 1995
37. 高壁穗，柑桔屬精油香味成分之探討，食品工業. 1994.
38. 竹內良二，柑橘系香料的最新趨勢，Beverage Japan,19(11):43-46. 1996.
39. 謝瑞忠，不同抽取方法對玉蘭花精油成分差異之研究，林業試驗所研究報告季刊. 1989.
40. Schieberle P.Grosch W. Quantitative Analysis of Important Volatile Flavor Compound in Fresh and Store Lemon Oil/Citric Acid Emulsion. Lebensm-Wiss u Technol, 21 158-162. 1988.
41. 李志雄，台灣柑橘類植物葉油成分之研究，國立台灣大學化學研究所碩士論文. 1980.
42. 謝瑞忠，二氧化碳超臨界流體方法抽取錫蘭肉桂葉香精研究，台灣林業科學. 1997.
43. 小林光廣，香味萃取與超臨界流體萃取技術，New Food Industry,32(11) 21-25. 1990.
44. 王美苓，橘皮精油、色素及植物固醇之研究，國立台灣大學農業化學所1990.
45. 李敏雄、柳煌、蘇南維等，柑橘皮自體酵素對精油中d-limonene轉換作用之研究，中國農業化學誌. 1999.
46. 王美苓、鍾玉明、李敏雄，萃取方法對橘皮精油定量之影響，中國農業化學誌. 1994.
47. 胡英著.近代化工熱力學.上海科技文獻出版社.1994
48. Hannay J. B. and Hogarth J., Proc.Roy.Soc.29:324.1879.
49. Hannay J. B., Proc.Roy.Sco.30:484. 1880.
50. Villard P.J., Phys.5:455. 1896.
51. Prins A.Acad., Sci.Amsterdam.17:1095. 1915.
52. Pilat S., Godlewicz M.US 2 188 012
53. Katz D. L.and Kurate F.,Ind.Eng.Chem.32:817. 1940.
54. K.Stephan and K.Lucas,Viscosity of Dense Fluid,Press,New York. 1979.
55. E.Klesper, Angew.Chem.Int.Engl.,17(16978)738.
56. R.C.Reid,J. M. Prausnitz and B.E.Poling,The Properties of Gases and Liquids,4th Ed.,McGraw-Hill,New York. 1987.
57. 吳俊毅,以超臨界二氧化碳萃取海豹油之研究,國立成功大學化學研究所碩士論文. 2003.
58. 李建助，由蜂蠟中萃取高級醇之研究，國立成功大學化學研究所碩士論文. 2005.
59. 中井資原著,張本義編譯，Crystal analysis Theory and Practice,復漢出版社.1990.
60. 賴慧芬 ，荖葉萃取液對黑色素生合成作用之研究，嘉南藥理科技大學生物科技研究所. 2003.
61. 張麗卿，化粧品製造實務.臺灣復文興業股份有限公司.1998
62. 洪偉章，陳榮秀.化粧品學. 高立圖書有限公司.2002