簡易檢索 / 詳目顯示

研究生: 簡清富
Chien, Ching-Fu
論文名稱: 運動素於外加力負載下之運動分析
The analysis of the motion of kinesin with external load
指導教授: 黃明哲
Huang, Ming-Jer
學位類別: 碩士
Master
系所名稱: 工學院 - 工程科學系
Department of Engineering Science
論文出版年: 2003
畢業學年度: 91
語文別: 中文
論文頁數: 71
中文關鍵詞: 運動素分子馬達
外文關鍵詞: molecular motor, kinesin
相關次數: 點閱:115下載:2
分享至:
查詢本校圖書館目錄 查詢臺灣博碩士論文知識加值系統 勘誤回報
  • 運動素(kinesin)為一種水溶性的酵素蛋白質分子,位於細胞內,依附於微管(microtubule)做單方向的前進。運動素的頭部與核酸鍵結的方式,將主導其運動機制。
    本文用擴散方程式結合馬可夫鏈推移矩陣,建立一個數學量化的模型,分別以v、L、r及 Ds這四個重要參數分析運動素處於負載下,沿著微管運動的機制,並與實驗數據做比較。
    由本文研究結果顯示,運動素在不同ATP濃度下,處於低負載時,速度無明顯變化。但處於低濃度時,r值會得到比較高的值,代表此時的ATP水解,是無法與運動素做鍵結,導致化學能量無法轉換成機械能。

    none

    摘要……………………………………………………………………Ⅰ 誌謝……………………………………………………………………Ⅱ 目錄……………………………………………………………………Ⅲ 表目錄…………………………………………………………………Ⅵ 圖目錄…………………………………………………………………Ⅶ 符號說明………………………………………………………………Ⅹ 第一章 緒論 1-1 前言…………………………………………………….1 1-2 研究動機……………………………………………….2 1-3 研究目的……………………………………………….3 1-4 文獻回顧……………………………………………….3 1-5 本文架構……………………………………………….4 第二章 生物分子馬達及運動素運動機制簡介 2-1 生物分子馬達簡介………………………………………………5 2-1-1 擺動生物分子馬達……………………………………………5 2-1-2 動力蛋白生物分子馬達………………………………………6 2-1-3 F1-ATPase旋轉生物分子馬達………………………………7 2-1-4 肌凝蛋白(myosin)生物分子馬達…………………………8 2-2 運動素運動機制…………………………………………………9 2-2-1 運動素與微管結構形狀………………………………………9 2-2-2 運動素運動的基本原理………………………………………10 2-2-3 運動素運動的基本機械-化學模型…………………………11 第三章 運動素運動模型建立及數學理論分析 3-1 模型敘述…………………………………………………………14 3-1-1運動素模型敘述…………………………………………………14 3-1-2 本文模型敘述…………………………………………………16 3-2 運動素運動模型…………………………………………………18 3-3數學理論分析………………………………………………………22 第四章 討論與結果 4-1 v分布……………………………………………………………… 30 4-2 Ds分布………………………………………………………………32 4-3 L分布……………………………………………………………… 33 4-4 r分布……………………………………………………………… 33 第五章 結論與未來研究方向 5-1 結論…………………………………………………………………36 5-2未來研究方向……………………………………………………… 37 參考文獻…………………………………………………………………38

    1.S. M. Block, L. S. Goldstein and B. J. Schnapp , “Bead movement by single kinesin molecular with optical tweezers”, Nature 48, pp. 348- 352, 1990

    2.C. Peskin, G. Odell and G. Oster, “Cellular motions and thermal fluctuations: the Brownian rachet”, Biophys. J. 65, pp. 316-324, 1993

    3.C. Peskin and G. Oster, “Coordinated hydrolysis explains the mechanical behavior of kinesin”, Biophys. J. 68, pp. 202-211, 1995

    4.C. M. Coppin, D. W. Pierce, L. Hsu and R. D. Vale, “ The load dependence of kinesin mechanical cycle”, Proc. Natl Acad. Sci. U.S.A. 94, pp. 6775-6779, 1997

    5.K. Visscher, M. J. Schnitzer and S. M. Block, “Single kinesin molecules studied with a molecular force clamp”, Nature, 400, pp. 184-189, 1999

    6.S. Rice, S. Rice, A. W. Lin, D. Safer, C. L. Hart, N. Naberk, B. O. Carragher, S. M. Cain, E. Pechatnikova, E. M. Wilson-Kubalek, M. Whittaker, E. PateI, R. Cookek, E. W. Taylor, R. A. Milligan and R. D. Vale, “A structural change in the kinesin motor protein that drives motility”, Nature 402, pp. 778-784, 1999

    7.M. J. Schnitzer, Visscher, K. and S. M. Block, “Force production by single kinesin motors”, Nature Cell Biol. 2, pp. 718-723, 2000

    8.S. M. Block, “Kinesin:What give ? ”, Cell, Vol. 93, pp. 5-8, 1998

    9.R. Vale, and R. Milligan, “The way things move:looking under the hood of molecular motor proteins”, Science. 288, pp. 88-95, 2000

    10.R. Vale, and R. Fletterick, “The design plan of kinesin motors”, Annu. Rev. Cell. Dev. Biol. 13, pp. 745-777, 1997

    11.W. Wriggers and K. Schulten, “Nucleotide-dependent movements of the kinesin motor domain predicted by Simulated Annealing”, Biophs J. 75, pp. 646-661, 1998

    12.D. L. Coy, M. Wagenbach and J. Howard, “Kinesin takes one 8-nm step for each ATP that it is hydrolyzes”, J. Biol. Chem. 274, pp. 3667-3761, 1999

    13.M. L. Moyer, S. P. Gilbert, and K. A. Johnson, “Pathway of ATP hydrolysis by monomeric and dimeric kinesin”, Biochemistry. 37, 800-813, 1998

    14.R. B. Case, S. Pierce, and C. L. Har, “Role of the kinesin neck linker and catalytic core in microtubule based motility”, Curr. Bio. 140, pp. 1407-1416 , 2000

    15.M. Fisher and A. Kolomesky, “The force exerted by a molecular motor”, Proc. Natl Acad. Sci. U.S.A. 93, pp. 6597-6602, 1999

    16.T. Elston, “A macroscopic description of biomelecular transport”, J. Math. Biol. 41, pp. 189-206, 2000

    17.N. G. Vankmpen, Stochastic process in physics and hemistry, Elsevier Science Publishers, Amsterdam. , 1987

    18.K. Svoboda and S. M. Block, “Force and velocity measured for single molecules”, Cell 77, pp. 773-784, 1994

    19.M. Fisher and A. Kolomesky, “Simple mechano-chemistry describes the dynamics of kinesin moleculars”, Proc. Natl Acad. Sci. U.S.A. 93, pp. 7748-7753, 2001

    下載圖示 校內:2005-08-22公開
    校外:2005-08-22公開
    QR CODE