本研究探討應用ELISA(Enzyme-linked Immunosorbent Assay)快速篩檢法於戴奧辛高污染土壤之整治調查研究。研究分兩部份進行，第一部分為建立ELISA分析方法的應用及找出其最適化操作條件。第二部分為使用ELISA快速篩試戴奧辛高污染土壤，再輔以高解析氣相層析儀/高解析質譜儀(High resolution gas chromatograph/high resolution mass spectrometer, HRGC/HRMS)進行確認求得校正因子，以便後續污染整治之利用。
研究結果顯示，最適化萃取時間為2個小時，即可達到從土壤樣品中置換出戴奧辛濃度之最大值；在淨化過程中，最適化甲苯淋洗量為20毫升，即可將活性碳管柱中淋洗出戴奧辛濃度最大值。此外，實場應用部分，採樣規劃所得樣品數量為78個樣品，但須以HRGC/HRMS分析其15%的樣品數量，進而求得校正因子(Calibration Adjustment Factor, CAF)。研究結果顯示，12個樣品經過CAF修正後，ELISA分析9個樣品呈現陽性反應，其中經HRGC/HRMS分析為7個樣品超過法規標準，ELISA分析亦呈陽性；此外將ELISA與HRGC/HRMS 共12組數據進行迴歸分析，得到其相關性R2=0.74，顯示兩者存在正相關性；成本估算由原先全數HRGC/HRMS分析改為ELISA分析，費用可省下719萬；分析時間亦是省下4個月。
實驗結果顯示應用ELISA可適當篩試出超標樣品，若運用於大面積污染調查，大量的樣品可先經由此方法找出其污染範圍及趨勢，必要時再將具有代表性的樣品進一步施做HRGC/HRMS分析。ELISA的分析不僅可節省分析費用、縮短時程、快速掌握污染分佈，更可運用於整治工作開始前之調查。

The research is to establish Enzyme-linked immunosorbent assay (ELISA) for contaminant soil and apply on dioxin contaminated soil. There are two phases in this study. Phase one is to establish the standard operation procedure of ELISA to obtain the optimal operation condition. Phase two is to apply ELISA of rapid screening on heavily dioxin contaminated soil. Meanwhile, the result of ELISA is confirmed by High resolution gas chromatograph/high resolution mass spectrometer (HRGC/HRMS) to obtain calibration adjustment factor (CAF)
The result shows that the optimal extraction time is 2 hours for concentration of dioxins reaching the maximum. While purification, the concentration of dioxins reaches the maximum from carbon column washed by 20 mL of Toluene. Moreover, in the part of application for the site, 12 of 78 samples have been analyzed by HRGC/HRMS for obtaining CAF. After correction of CAF. The results shows that 9 of 12 samples analyzed by ELISA screening test appear positive, and 7 of which exceed the regulatory standard in HRGC/HRMS analysis. Regression analysis is conducted to the 12 data measured by ELISA and HRGC/HRMS, and the r-square is 0.74, indicating a positive correlation between these two set of data. It is estimated 7.2 million dollars and 4 months can be saved if ELISA is used instead of HRGC/HRMS.
ELISA screening test can analyze Dioxin sample quickly. A large number of samples screening by ELISA can help to understand the distribution and trend of pollutant for the investigation in a large contaminated site. If it’s necessary, the representative samples could be analyzed by HRGC/HRMS. ELISA is not only saving money and time but also is a benefit for investigation.

Bank P. A., Yao E. F., Phelps C. L., Harper P. A., Denison M.S. (1992), “Species-specific binding of transformed Ah receptor to a dioxin responsive transcriptional enhancer,” European Journal of Pharmacology; Vol. 228 (2–3), pp. 85-94.
Barry C., Webster T. (1994), Overview: the dioxin debate. In: Schecter A. Dioxins & Health. Plenum Press, New York, pp. 1-50.
Behnisch P. A., Hosoe K., Sakai S. (2001), “Bioanalytical screening methods for dioxins and dioxin-like compounds a review of bioassay/biomarker technology,” Environment International; Vol. 5, pp. 413-439.
Bhavsar, S. P., Reiner, E. J., Hayton, A., Fletcher, R., and MacPherson, K., (2008), "Converting Toxic Equivalents (TEQ) of dioxins and dioxin-like compounds in fish from one Toxic Equivalency Factor (TEF) scheme to another," Environment international; Vol. 34 (7), pp. 915-921.
Bieber A. (1997).” Current Aspects to Face the Issues of Contaminated Land and Groundwater in Germany,” 第1回国際土壌・地下水環境ワークショップ講演集(社)土壌環境センタ.
CCME, Canadian Council of Ministers of the Environment (1991), “Interim Canadian Environmental quality Criteria for Contaminated Sites,” Report CCME EPC-cs34.
Connett P., Webster T. (1987), “An estimation of the relative human exposure to 2,3,7,8-TCDD emissions via inhalation and ingestion of cow’s milk,” Chemosphere; Vol. 16, pp. 2079-2084.
Cook J. C., Caido K. W., Greenlee W. F. (1987), “Ah receptor: relevance of mechanistic studies to human risk assessment,” Environ Health Perspectives; Vol. 76, pp. 71-77.
Czuczwa J. M., Niessen F., Hites R. A. (1985), “Historical record of polychlorinated dibenzo-p-dioxins and dibenzofurans in Swiss lake sediments,” Chemosphere; Vol. 14, pp. 1175-1179.
Czuczwa J, Hites R. (1986), “Airborne dioxins and dibenzofurans: Sources and fates,” Environmental Science & Technology; Vol. 20, pp. 195-200.
Denison M.S., Seidel S. D., Ziccardi M., Rogers W. J., Brown D. J., Clark G. C. (1999), “Ah receptor based bioassays: applications and limitations,” Organohalogen Compound; Vol. 40, pp. 27–30.
DETR, European Commission DG Environment UK Department of Environment Transport and the Regions (1999), “Compilation of EU Dioxin Exposure and Health Data summary report,” p. 9.
Dirkle Jr., Wolfe W. H., Patterson D. G. Jr., et al, (1989), “Estimates of the Half-Life of 2378-TCDD in Rarch Hand Veterans,” Journal of Toxicology and Environmental Health; Vol. 27 (2), pp. 165-171.
Environment Agency of Japan (1999), “Environment Monitoring Technology;” Vol.26 .No.12. pp. 2-8.
Environmental Protection Administration (EPA) (2001), “Control Standard of Soil Pollution,” HSSZ No.007368.
Farrell K., Safe L., Safe S. (1987), “Synthesis and AhR binding properties of radiolabeled PCDF congeners,” Archives of Biochemistry and Biophysics; Vol. 259, pp. 185–95.
Fishbein L. (1987), “Health-risk estimates for 2,3,7,8-tetrachlorodibenzo -p-dioxin : an overview,” Toxicology and Industrial Health; Vol. 3, pp. 91-134.
Fiedler H., Hutzinger O., Timms C. (1990), “Dioxins: Sources of environmental load and human exposure,” Toxicological and Environmental Chemistry; Vol. 29, pp. 157-234.
Flesch-Janys D., Becher H., Gurn P., Jung D., Konietzko J., Manz A., Papke O. (1996), “Elimination of polychlorinated dibenzo-p-dioxins and dibenzofurans in occupationally exposed persons,” Journal of Toxicology & Environmental Health ; Vol. 47, pp. 363-78.
Harrison R. O., Carlson R. E. (1999) “Immunoassay analysis of dioxin in soil: validation of TEQ screening at 500 ppt using rapid extraction clean up,” Organohalogen Compound; Vol. 40, pp. 31-34.
Hurst M. R., Balaam J., Chan-Man Y. L., Thain J. E., Thomas K. V. (2004), “Determination of dioxin and dioxin-like compounds in sediments from UK estuaries using a bio-analytical approach: chemical-activated luciferase expression (CALUX) assay,” Marine Pollution Bulletin; Vol. 49 (7-8), pp. 648-658.
Kennedy S. W., Lorenzen A., James C. A., Stegeman J. J. (1996), “Cytochrome P4501A induction in avian hepatocyte cultures: a promising approach for predicting the sensitivity of avian species to toxic effects of halogenated aromatic hydrocarbons,” Toxicology and Applied Pharmacology; Vol. 141, pp. 214–230.
Kopponen P., Törrönen R., Maeki-Paakkanen J., von Wright A., Kärenlampi S. (1994), “Comparison of CYP1A1 induction and genotoxicity in vitro as in-dicators of potentially harmful effects of environmental samples,” Archives of Toxicology ; Vol. 68, pp. 167–173.
Koppen G., Covaci A., Van Cleuvenbergen R., Schepens P., Wineke G., Nelen V., van Larebeke N., Vlietinck R., Schoeter G. (2002), “Persistent organochlorine pollutants in human serum of 50-65 years old women in the Flanders Environmental and Health Study (FLEHS).Part : concentrations and regional differences,” Chemosphere; Vol. 48, pp. 811-825.
Kuratsune M. Yusho, with reference to Yu-Cheng. In: Kimbrough R. D., Jensen A. A., eds. (1989), “Halogenated Biphenyls, Terphenyls, Naphthalenes, Dibenzodioxins and Related Products,” Elsevier, Amsterdam, pp. 381-400.
Liem A. K. D. (1999), “Dioxins and dioxin-like PCBs in food-stuffs. Levels and trends,” Organohalogen Compound; Vol. 44, pp. 1-4.
Malisch R. (1998), “Update of PCDD/PCDF-intake from food in Germany,” Chemosphere; Vol. 37, pp. 1687-98.
Marklund S., Andersson R., Tysklind M. (1990), “Emissions of PCDDs and PCDFs in gasoline and diesel fueled cars,” Chemosphere; Vol. 20, pp. 553-561.
May G. (1982), “Tetrachlorodibenzodioxin: a survey of subjects ten years after exposure,” British Journal of Industrial Medicine; Vol. 39, pp. 128-135.
McConnell E., Huff J., Hejtmancik M., Peters A., Persing R. (1991), “Toxicology and carcinogenesis studies of two grades of pentachlorophenol in B6C3F1 mice,” Fundamental and Applied Toxicology; Vol. 17, pp. 519-532.
Michalek J. E., Tripathi R. C., Caudill S. P., Pirkle J. L. (1992), “Investigation of TCDD half-life heterogeneity in veterans of Operation Ranch Hand,” Journal of Toxicology & Environmental Health; Vol. 35, pp. 29-38.
Ministry for the Environment (New Zealand) (1998), “Reporting on Persistent Organochlorines in New Zealand,” p.5.
Nelson E. A., Hui L. L., Wong T. W., Hedley A. J. (2006), “Demographic and lifestyle factors associated with dioxin-like activity (CALUX-TEQ) in human breast milk in Hong Kong,” Environmental Science & Technology.; Vol. 40 (5), pp. 1432-1438.
NUR, Naturschutz und Reaktorsicherheit, Bundesministerium für Umwelt (1993), “Berucht der Bund/Lander-Arbeitsgruppe Dioxin.”
Olson Jr. (1994), Pharmacokinetics of Dioxin and Related Chemicals. In: Schecter A., ed: Dioxins and Health. Plenum Press，New York.
Poiger H., Buser H. R., Schlatter C. (1984), “The metabolism of 2,3,7,8-tetrachlorodibenzofuran in the rat” Chemosphere; Vol. 13, pp. 351-357.
Parzefall, W. (2002), "Risk assessment of dioxin contamination in human food," Food and chemical toxicology; Vol. 40, No. 8, pp. 1185- 1189.
Riss A., Scheidl K. (1990), “Statutory and research action in Austria”, Presented at Dioxin’ 90, Bayreuth, Organohalogen Compounds; Vol. 4, pp. 51-56.
Randerath K., Zhou G. D., Randerath E., Safe S. H., Donnelly K. C. (1997), “Comparative 32P-postlabelling analysis of exogenous and endogenous DNA adducts in mouse skin exposed to a wood-preserving waste extract, a complex mixture of polycyclic and polychlorinated chemicals,” Environmental and Molecular Mutagenesis; Vol. 29, pp.372–378.
Rappe C. (1992), “Sources of PCDDs and PCDFs. Introduction: Reactions, levels, patterns, profiles and trends,” Chemosphere; Vol. 25, pp. 41-44.
Rejinders P., Brasseur S. (1992), “Xenobiotic induced hormonal and associated developmental disorder in marine organisms and related effects in humans: An overview,” In : Colborn T，Clement C, eds. Chemically-Induced Alterations in Sexual and Functional Development: The Wildlife/Human Connection，Princeton Scientific Publishing Co. Princeton NJ, pp. 159-174.
RIVM, Rijksinstituut voor Volksgezondheid en Milieu (1994), “Human Exposure to soil Contamination; a Qualitative and Quantitative Analysis towards Proposals for Human Toxicological C-standard Values,” Report No. 75201006.
Ross, Peter S. & Birnbaum, Linda S. (2003), “Integrated Human and Ecological Risk Assessment: A Case Study of Persistent Organic Pollutants (POPs) in Humans and Wildlife,” Human and Ecological Risk Assessment: An International Journal; Vol. 9(1), pp. 303-324.
Safe S. (1984), “Polychlorinated biphenyls (PCBs) and polybrominated biphenyls(PBBs): biochemistry, toxicology and mechanism of action,” Critical reviews in toxicology; Vol. 12, pp.319–395.
Safe S. H. (1986), “Comparative toxicology and mechanism of action of polychlori-nated dibenzo-p-dioxins and dibenzofurans,” Annual Review of Pharmacology and Toxicology; Vol. 26, pp.371–399.
Safe S. (1987), “Determination of TEFs: support for the use of the in vitro AHH induction assay,” Chemosphere; Vol. 16(4), pp.791–802.
Safe S. H. (1990), “PCBs, PCDDs, PCDFs and related compounds: environmental and mechanistic considerations which support the development of toxic equivalency factors (TEFs),” Critical Reviews in Toxicology; Vol. 21(1), pp.51–87.
Safe S. (1993), “Development of bioassays and approaches for the risk assessment of 2,3,7,8-tetrachlorodibenzo-p-dioxin and related compounds,” Environmental Health Perspectives;101(Suppl 3), pp.317–25.
Safe S. H. (1994), “Polychlorinated biphenyl (PCBs). Environmental impact, bio-chemical and toxic responses, and implications for risk assessment,” Critical Reviews in Toxicology; Vol. 24(2), pp.87–149.
Safe S. (1997), “Limitations of the TEF approach for risk assessment of TCDD and related compounds,” Teratogenesis, Carcinogenesis, and Mutagenesis; Vol. 17, pp.285–304.
Safe S. H. (1998a), “Development validation and problems with the TEF approach for risk assessment of dioxins and related compounds,” Journal of Animal Science; Vol. 76, pp.134–41.
Safe S. H. (1998b), “Hazard and risk assessment of chemical mixtures using toxic equivalency factor approach,” Environmental Health Perspectives; 106(Suppl 4), pp.1051–8.
Safe S., Mason G., Farrell K., Keys B., Piskorska-Pliszczcynska J., Madge J. A., Chittim B. (1987), “Validation of in vitro bioassays for 2,3,7,8-TCDD equivalents,” Chemosphere; Vol. 16, pp.1723–8.
Safe S., Mason G., Sawyer T., Zacharewski T., Harris M., Yao C., Keys B., Farrell K., Holcomb M., Davis D., Safe L., Piskorska-Pliszczynska J., Leece B., Denomme M. A., Hutzinger O., Thoma H., Chittim B., Madge J. (1989), “Development and validation of in vitro induction assays for toxic halogenated aromatic mixtures: a review,” Toxicology and Industrial Health; Vol. 5, pp.757–75.
Safe S., Harris M., Zacharewski T. (1991), Development and validation of bio-assays for polychlorinated dibenzo-p-dioxins and dibenzofurans, International Agency for Research on Cancer (IARC) Science Publications; Vol. 108, pp.147–59 (Chapter 6).
Safe S., Connor K., Ramaoorthy K., Gaido K., Mannes S. (1997), “Human exposure to endocrine-active chemicals: hazard assessment problems,” Regulatory Toxicology and Pharmacology; Vol. 26, pp.52–8.
Sanderson J. T., Kennedy S. W., Giesy J. P. (1998), “In vitro induction of ethoxyresorufin-O-deethylase and porphyrins by halogenated aromatic hydrocar-bons in avian primary hepatocytes,” Environmental Toxicology and Chemistry; Vol. 17(10), pp.2006–18.
Schartz P., Jacobson S., Fein G., et al. (1983), “Lake Michigan fish consumption as a source of polychlorinated biphenyl in human cord serum，maternal serum and milk,” American Journal of Public Health; Vol. 73, pp. 293-296.
Schecter A., Ryan J. J., Constable J. D. (1986), “Chlorinated dibenzo-p-dioxin and dibenzofuran levels in human adipose tissue and milk samples from the north and south of Vietnam,” Chemosphere; Vol. 15, pp. 1613-1620.
Schecter A. (1994), Dioxins and health. New York: Plenum.
Sinkkonen, S., Paasivirta, J. (2000), “Degradation half-life times of PCDDs, PCDFs and PCBs for environmental fate modeling,” Chemosphere; Vol. 40, pp. 943–949
Schoeters G., Goyvaerts M. P., Ooms D., Van Cleuvenbergen R. (2004), “The evaluation of dioxin and dioxin-like contaminants in selected food samples obtained from the Belgian market: comparison of TEQ measurements obtained through the CALUX bioassay with congener specific chemical analyses,” Chemosphere; Vol. 54 (9), pp. 1289-97.
Schrenk D., Lipp H. P., Wiesmüller T., Hagenmaier H., Bock K. W. (1991), “Assessment of biological activities of mixtures of polychlorinated dibenzo-p-dioxins: comparison between defined mixtures and their constituents,” Archives of Toxicology; Vol. 65, pp.114–8.
Schwirzer S. M. G., Hofmaier A. M., Kettrup A., Nerdinger P. E., Schramm K. W., Thoma H., Wegenke M., Wiebel F. J. (1998), “Establishment of a simple cleanup procedure and bioassay for determining 2,3,7,8,-tetrachlorodibenzo-p-dioxin toxicity equivalents of environmental samples,” Ecotoxicology and Environmental Safety; Vol. 41, pp. 77–82.
Swedish EPA (1996), “Development of Generic Guideline Values.”
Takigami H., Matsuda T., Matsui S., Yamada M. (1999), “Screening of AhR ligands in the environment using the recombinant yeast,” Abstract book of the 2nd meeting of the Japan Society of Endocrine Disrupter Research International Symposium on Environmental Endocrine Disruptors 1999, Japan Society of Endocrine Disrupters Research, Tsukuba, Japan; p. 87 (Organized by Environment Agency, Government of Japan).
Tillitt D. E., Gale R. W., Meadows J. C., Zajicek J. L., Peterman P. H., Heaton S. N., Jones P. D., Bursian S. J., Kubiak T. J., Giesy J. P., Aulerich R. J. (1996), “Dietary exposure of mink to carp from Saginaw Bay: 3. Characterisation of dietary exposure to planar halogenated hydrocarbons, dioxin equivalents and biomagnification,” Environmental Science & Technology; Vol. 30, pp.283–91.
Tysklind M., Tillitt D., Eriksson L., Lundgren K., Rappe C. (1994), “A toxic equivalency factor scale for polychlorinated dibenzofurans,” Journal of Applied Toxicology; Vol. 22, pp.277–85.
U. S. EPA, (1998), “Approach for Addressing Dioxin soil at CERCLA and RCRA Sites,” OSWER Directive 9200.
U.S. EPA. (2000), “Exposure and Human Health Reassessment of 2,3,7,8 -Tetrachlorodibenzo-p-Dioxin (TCDD) and Related Compounds. Part I: Estimating Exposure to Dioxin-Like Compounds,” Volume 3: Properties, Environmental Levels, and Background Exposures
U.S. EPA, (2002) “Screening for polychlorinated dibenzodioxins and polychlorinated dibenzofurans (PCDD/Fs) by immunoassay,” Method 4405.
U.S. EPA (2003), "Exposure and Human Health Reassessment of 2,3,7,8-Tetrachlorodibenzo-p-Dioxin (TCDD) and Related Compounds National Academy Sciences (NAS) Review Draft,Part I: Estimating Exposure to Dioxin-Like Compounds,” Volume 2: Properties, Environmental Levels, and Background Exposures.
U.S. EPA, (2007). “Interim report on the evolution and performance of the Eichrom Technologies Procept® rapid dioxin assay for soil and sediment samples,” EPA/540/R-07/001.
U.S. EPA, (2007). “Screening extracts of environmental samples for planar organic compounds (PAHs, PCBs, PCDDs/PCDFs) by a reporter gene on a human cell line.” February.
U.S. EPA, (2008). “Performance of the CAPE Technologies DF1 dioxin/furan immunoassay kit for soil and sediment samples.” EPA/540/R-08/002, February.
Van der Molen G. W., Kooijman S. A., Slob W. (1996), “A generic toxicokinetic model for persistent lipophilic compounds in humans: an application to TCDD,” Fundamental & Applied Toxicology; Vol. 31, pp. 83-94.
Van Pelt F. N., Hassing I. G., Stelling M. A., Seinen W., Blauboer B. J. (1992), “Induction of terminal differentiation in cultured human keratinocytes by polychlorinated aromatic hydrocarbons as measured by cell size analysis,” Toxicology and Applied Pharmacology; Vol. 113(2), pp.240–245.
Wolfe W. H., Michalek J. E., Miner J. C., Pirkle J. L., Caudill S. P., Patterson D. G. Jr., Needham L. L. (1994), “Determinants of TCDD half-life in veterans of operation ranch hand,” Journal of Toxicology & Environmental Health; Vol. 41, pp. 481-488.
Ziccardi M. H., Gardner I. A., Denison M. S. (2000), “Development and modification of a recombinant cell bioassay to directly detect halogenated and polycyclic aromatic hydrocarbons in serum,” Toxicological Sciences; Vol. 54, pp.183–93.
Zoric M., Sjolund M., Persson M., Nilsson E., Lundeheim N., Wallgren P. (2004), “Lameness in Piglets. Abrasion in Nursing Piglets and Transfer of Protection towards Infection with Streptococci from Sow to Offspring,” Journal of Veterinary Medicine; Vol. 51, pp. 278 – 284
日本環境廳(1999)，環境的測定技術.Vol.26 .No.12.pp.2-8.
洪慧倫(2007)，「台灣戴奧辛物質流分析」，國立台灣大學環境工程學研究所碩士論文。
行政院環境保護署(2001)，環署水字第○○七三六八，「土壤污染管制標準」。
中央研究院近代史研究所檔案館(1956)，「台灣鹼業公司民國45年度工作報告」。
行政院環境保護署環境檢驗所(2005)，「戴奧辛生物快速篩選技術及應用（I）計畫報告」。
徐慈鴻, 李貽華, 高清文(2008)，「戴奧辛生物快速篩檢法在農、畜及水產品的應用」，行政院農委會藥毒所技術; Vol. 167。
陳元武(2010)，「開發本土戴奧辛生物快速篩選法的重要性」，生物化學酵素冷光基因表現法技術研討會。