普利醇（Policosanol）又稱甘蔗原素，是由含C24~C34等高級脂肪醇之混合物，其中以二十八醇為主，一般存在於蔗蠟、蜂蠟、蟲蠟及小麥胚芽油等天然物質中。經研究發現普利醇對人類具有增強體力及治療等作用，漸為世人所重視，是一種具獨特生物活性之物質。
超臨界流體萃取技術，具有無毒、乾淨、省能源及符合綠色環保之特性，無傳統有機溶劑之殘留及污染之問題，且可利用溫度或壓力的變化來調整流體的性質，增加其應用性。對溫度熱敏感容易氧化變質的食品及傳統藥理有效成份的萃取，不會有變質變性之缺點。
本研究乃將蜂蠟先經皂化（saponification）後，利用超臨界二氧化碳流體技術與傳統溶劑萃取，將蜂蠟中之高級脂肪醇類萃取分離，並進行萃出率與純度之比較。在改變實驗條件如改變靜態萃取時間、溫度、壓力、添加共溶劑等變因下，探討其對萃取效率之影響，並得到最佳萃取條件為以溫度55 ℃,壓力為3000 psi，乙醇為共溶劑(w/v, g/mL 1:20)，靜置時間為60 min，萃出率為11.2％，高級醇含量值為30(mg/g)。

Policosanol is a natural mixture of C24~C34 aliphatic primary alcohols in which octacosanol is the major component existing in sugarcane wax, beeswax, insect wax and wheat germ oil. It was found that Policosanol is able of increasing the strength and metabolic activity of muscles, and has been developed as a food supplement.
CO2 has been widely used in the Supercritical Fluid Extraction System. Not only does it show low viscosity, high diffusion, non pollution, non flammability but also high selectivity of excellent performance. By changing the temperature and pressure in the Supercritical CO2 extraction, it displays different extraction characteristic. It also could prevent from denature of the thermo-sensitivity ingredients of the food and pharmaceuticals during the extraction.
In this study, SFC extraction and traditional solvent extraction were used to extract the aliphatic primary alcohols from saponified Beeswax. The extraction efficiency and purity of the alcohol obtained using these two methods were compared. The effectives of the time, temperature, pressure and cosolvent were also the variant used to evaluate the extraction efficiency. It was found that the best condition of SFC extraction is at 55℃, 3000psi, using ethanol as the cosovent (w/v, g/mL 1:20) and 60 min of mixing time in supercritical CO2 of liquid extraction. The content of Policosanol is 30 mg/g in the beeswax and the extraction yield is 11.2%.

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