痛風(gout)為一種關節性病變，起因於體內嘌呤(purine)代謝異常，導致高尿酸血症，而使尿酸鈣鹽沈積在關節腔內，造成關節劇烈疼痛及腫脹的疾病，而痛風病患應避免進食含高量嘌呤的食物或飲料，如海產、啤酒等，尤其啤酒特別容易誘發痛風。
　　本研究中以不同去乙醯度及分子量之幾丁聚醣(chitosan)吸附啤酒中之嘌呤，希望能減少啤酒中嘌呤之含量。本實驗利用過氯酸水解出啤酒中之嘌呤，並以高效能液相層析儀分析。結果顯示幾丁聚醣可以有效吸附啤酒中之嘌呤，而且分子量愈小、去乙醯度愈高之幾丁聚醣其吸附效果愈好，以分子量為8.8萬、去乙醯度為90% 之幾丁聚醣為例，將0.4g幾丁聚醣置入10ml啤酒中，於60分鐘內可吸附啤酒中約71% 之嘌呤。本研究之結論顯示幾丁聚醣可以有效減少啤酒中嘌呤之含量，幾丁聚醣含量愈多、去乙醯度愈高、分子量愈小，吸附啤酒中之嘌呤效果愈好，分子量對吸附作用之影響尤其顯著。

　　Gout is one of the most common forms of arthritis (joint inflammation). When the uric acid cannot be completely filtrated by kidney, it can form monosodium urate crystals and accumulate in the joints and form gout. The gout patients and moderate alcohol drinkers always need to select foods cautiously to avoid the foods or beverages that contain high concentration of purine.
Beers contain high concentration of purine. The purpose of this study is to use chitosan to adsorb the purine in beer to reduce beer’s purine content. The purine content in beer was measured by HClO4 hydrolysis method and analyzed by reverse phase HPLC method. Our data showed that chitosan reduce beer’s purine content in a dose response manner. The smaller molecule weight and higher degree of deacetylation (DD) value of chitosan had better absorptive ability. When chitosan that DD value of 90% and Mv of 88,000 at concentration of 0.4g/10ml, it reduced total beer’s purine content by 71.1%. This report demonstrates that chitosan can be used to adsorb purine and reduce purine concentration in beer.

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