四草曾經為台灣著名的產鹽區，但隨著產業的式微，四草在這數十年時間已荒廢了大量鹽田，在這段時間內不同的地點有不同的動、植物進駐，而仍有許多廢棄鹽田屬於高鹽極端環境，為了探究當地微生物組成、生態意義以作為鹽田復育相關的微生物指標。
本研究選定四個時間點採集了六個不同樣點的鹽田環境，四個土樣、兩個水樣，假定這六個樣點分別為不同演替速度所造成的結果，而將這些樣本分別萃取出DNA後以16SrRNA序列作為分子條碼，探究各樣點的微生物多樣性。樣本由16S-15/884、16S-789/1053、16S-926/1392等引子經PCR增幅後，以Illumina MiSeq system 進行次世代定序（Next-generation sequencing），所得的序列經程式篩選、BLASTClust集結產生OTUs（Operational taxonomic units）及其編號，對資料庫進行比對後得到物種資訊，再利用MEGAN軟體分析樣點中已鑑定出的類群的相對百分比豐度及各樣點的alpha多樣性，計算其Bray-Curtis dissimilarity值後藉由R程式中的階層分析（Cluster dendrogram）來評估群落物種之間分佈的相似程度。
最後結果顯示有符合演替趨勢隨之增加的在phylum階層有Acidobacteria、Actinobacteria、Gemmatimonadetes、Chloroflexi、Gemmatimonadetes、Nitrospirae、Verrucomicrobia，反之有下降趨勢的有Spirochaetes、Lentisphaerae。而在一般觀光鹽田（仍持續曬鹽）的優勢屬有Truepera、Halothermothrix、Geobacillus廢棄鹽田(旁有蘆葦）的有Geotoga、Halocella、Caldilinea，池邊（少植物生長）的有Thiohalophilus、Dasania、Thioalkalivibrio，池邊（多植物生長）的有Thiohalophilus、Burkholderia、Thiobacter，目前仍有許多優勢菌屬仍不清楚會對該生態如何造成影響，有待更進一步的研究，而符合趨勢的菌更可作為在高鹽逆境環境微生物策略復育時之參考。

In this study, the salterns of Sutzao, Tainan were chosen for research. Saltern is a place for salt making, and it used to be a prosperous industry in Sutzao. These abandoned areas have now been transformed into a protected area by the government for educational, research, and sightseeing purposes. In previous studies have indicated that ecological restoration is highly related to plants, because their roots can eliminate and confine harmful chemicals in the soil. In this paper, we strived to find important genera related to or even indicators of environmental change in the abandoned saltern. The following are the approaches implemented to understand the saltern’s microbial community and their changing pattern: Illumina sequencing machine for next generation sequencing (NGS), PERL, and MEtaGenome Analyzer version4.70.4, R project for clustering dendrogram. The results show the taxonomy phylum Acidobacteria、Actinobacteria、Gemmatimonadetes show an increasing pattern by succession. On the other hand, Spirochaetes displayed a decreasing pattern. In this research, we found that several genera play an important role in the ecological system. Zoogloea is used in waste water treatment for its ability to lower biological oxygen demand and promote the formation of sludge deposits. Marinobacter grows at NaCl concentrations of 0.08 to 3.5 M and uses various hydrocarbons as the sole source of carbon and energy. Burkholderia, Nitrosospira are common bacteria in soil or rhizosphere, and are associated with carbon and nitrogen cycle, respectively. Still, further research is needed, there are still many beneficial genera, but how they affect local ecology remains unclear. However, some of them show the trend of saltern's succession. These genera may be used as an alternative indicator of high salt adversity to monitor microbial communities’ changes resulting from human or natural disturbances.

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