蛋白質樣品的上機前處理是整個蛋白質組分析流程中必不可少的步驟，在以傳統的蛋白質純化的實驗過程中所使用的有機溶劑和帶有腐蝕性的酸性物質經常帶來環境的危害，多數耗材以及廢液難以回收再利用，因此開發一個符合綠色化學概念的純化過程是我們希望達到的目標。
我們在文中探討了目前在生物分子提取純化應用方面熱門的綠色溶劑，而其中基於膽鹼鹽以及三甲基甘氨酸的深層共晶溶劑提供的熱穩定性質以及幫助蛋白保持天然構型，避免失去天然結構的特性，不但能夠避免蛋白之間非期望的共價聚集，也十分有利於生物分子的提取，在本文中以多種可再生天然物來構成的親水性和疏水性深層共晶溶劑以液-液均相萃取法分別對於不同蛋白純化方面的評估；在親水性深層共晶的部分，相較於探討萃取效率，我們更專注於探討萃取後的DES層中的蛋白如何提升反萃取的效率，在實驗流程中探討了加入乙醇，硫酸銨，聚乙二醇以及調整pH值，溫度在反萃取方面的可能性，並將此反萃取應用於血漿應用的方面，使在血漿樣品最高豐度的蛋白:白蛋白，轉鐵蛋白以及IgG可以大幅度的去除，藉此使其他較低豐度的蛋白占比得以提升。
此外，接續實驗室過去的實驗，已知白蛋白以及組蛋白上修飾醌化雌激素會在500nm波長激發後，於595nm波長處發出強烈的螢光，利用這個特性，我們可以利用SDS-PAGE上的螢光條帶訊號以及螢光圖譜來追蹤修飾醌化雌激素蛋白加成物的訊號，已知DES萃取的機制與蛋白的表面性質有關連，醌化雌激素的修飾將使蛋白表面的部分位點變得較為疏水，於是有了用疏水性DES萃取醌化雌激素的想法，藉由使用3種不同以薄荷醇，百里酚為設計主軸的低黏滯性的疏水性DES來萃取上述樣品，設計一個可以將醌化雌激素蛋白加成物分離出來的實驗流程。

The purpose of this thesis is to explore the application of deep eutectic solvent. To enable the detection of the signal belongs to low abundance protein in plasma, the depletion of high abundance protein is an essential step, during whole proteomics. We found that after protein extraction with hydrophilic DES, high abundance protein can be removed by adding PEG-ammonium sulfate in back-extraction process. In another part, fluorescence spectra proved that the hydrophobic DES which composed with betaine and thymol has a potential to make 4-OHE2-proteins be enriched.

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