本論文利用熱裂解法及共沉澱法合成氧化鐵奈米粒子，並藉碳二醯胺活化作用將伴刀豆球蛋白(Con A)固定於其表面，製得磁可操控之生醫奈米載體，以用於捕捉病毒及分離醣蛋白。
熱裂解法可合成出單分散且粒徑均一的氧化鐵奈米粒子，平均粒徑約7.1±0.7 nm。以DMSA親水化後將Con A固定化於其表面，TEM影像顯示Con A已成功被覆在氧化鐵奈米粒子表面。但當Con A濃度較高時，會因分子間的交聯而引發團聚現象。由共沉澱法所合成之氧化鐵奈米粒子平均粒徑約13.3±5.2 nm，固定化Con A後，可由其FTIR光譜所示之醯胺鍵特性峰證實Con A已成功被覆在其表面，由TGA量測可決定Con A固定化的量。
將製得之Con A-Fe3O4奈米粒子以酵素免疫分析法 (ELISA) 檢測其與流感病毒H1N1之結合，結果顯示固定化之Con A仍然維持其活性並能捕捉病毒，且其於pH 8環境下捕捉病毒的能力略優於在pH 5的環境下，透過TEM分析可觀察到氧化鐵與病毒結合之情況。此外，本研究也將製得之Con A-Fe3O4奈米粒子用於醣蛋白的分離，證實非醣蛋白之牛血清蛋白無法被吸附，而屬於醣蛋白之大豆凝集素則可被吸附。

In this thesis, iron oxide nanoparticles (Fe3O4 NPs) have been synthesized by both co-precipitation method and thermal decomposition method. Concanavalin A(Con A) was immobilized on their surface via carbodiimide activation to obtain the magnetically manipulable biomedical nanocarrier for the capture of virus and the separation of glycoprotein.
By thermal decomposition method, monodisperse and uniform Fe3O4 NPs with a mean diameter of 7.1±0.7 nm were synthesized. After modified with DMSA, Con A was immobilized on their surface. From TEM image, the coating of Con A on the surface of Fe3O4 NPs could be demonstrated. When Con A concentration was too high, molecular interaction might result in the aggregation. The Fe3O4 NPs obtained by co-precipitation method had a mean diameter of 13.3±5.2 nm. After the immobilization of Con A, the characteristic peak owing to amide group in the FTIR spectrum demonstrated the successful coating of Con A. By TGA, the amount of Con A immobilized was determined.
The conjugation of Con A-Fe3O4 NPs with influenza virus H1N1 was detected by enzyme-linked immunosorbent assay (ELISA). It was found that the Con A immobilized retained its activity and could capture the virus. The capture at pH 8 was slightly higher than that at pH 5. By TEM analysis, the binding of Con A-Fe3O4 NPs onto the surface of virus could be demonstrated. In addition, the resultant Con A-Fe3O4 NPs were also used for the separation of glycoprotein. It was demonstrated that the non-glycoprotein borine serum albumin (BSA) was not adsorbed but the glycoprotein soybean lectin could be adsorbed.

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