細胞外間質(ECM)是人體組織中非常重要的成分，除了可以維持細胞結構，也有調節細胞行為的作用，包含細胞排列、黏附、遷移和分化。細胞外間質由許多不同類型的蛋白質組成，其中第一型膠原蛋白是ECM中最豐富的成分，在體內會形成複雜且緻密的多層結構。為了構建用於細胞培養和生物醫學應用(例如組織再生和傷口修復)的生物界面，在研究中我們探索了第一型膠原蛋白在生物相容性二維材料(雲母)上的自組裝現象，更進一步利用空間侷限的方法創建了基於膠原蛋白的生物界面，以模擬細胞生長的生存環境。
雲母是由鈉、鉀、鎂和其他金屬鋁矽酸鹽組成的原子級平面層狀基材，當溶液與雲母片接觸時，這些離子會從表面離解。已有多篇文獻討論了各種陰離子對雲母上第一型膠原蛋白自組裝的影響，因此本研究將著重探討陽離子效應，以及陽離子濃度、離子半徑、離子電荷對第一型膠原蛋白自組裝成層級式微奈米結構的影響。另外，也會討論影響第一型膠原蛋白自組裝成奈米纖維的雲母晶格引導，以及緩衝溶液的pH值和電解質的效應。

The extracellular matrix (ECM) is the most important component in the tissue, which can not only maintain cell structures, but also regulate cell behaviors, including cell alignment, adhesion, migration and differentiation. The ECM is composed of different types of proteins among which type I collagen is the most abundant component, forming complex and dense multilayered structures as biointerfaces in tissues. In order to construct biointerfaces for cell culture and biomedical applications, such as tissue regeneration and wound repair, we explored the self-assembly of type I collagen on a biocompatible two-dimensional material (mica), and further created a collagen-based biointerface that can simulate the living environment for cell growth in this research. Mica is an atomically flat and layered substrate composed of sodium, potassium, magnesium and other metal aluminosilicates, which ions dissociate from the surface when it comes in contact with solution. Several literatures have discussed the effects of various anions on collagen self-assembled on mica, so this research will focus on exploring the cation effect as well as the influence of cation concentration, ionic radius, and ionic charge on the self-assembly of type I collagen into a hierarchical micro-nanostructure. Lattice guidance of mica for self-assembly of collagen into nanofibers, the pH value of the buffer solution and the electrolyte effects on arrangement of type I collagen nanofibers will be discussed as well.

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