真空輔助切片術為目前普遍被使用的微創手術，其取樣方法以旋轉切削法為主。若在取樣過程中切削力過大，會過度擠壓組織，導致樣本破碎、質量過小等樣本品質問題，影響診斷的準確度。因此，許多文獻，探討如何降低軸向切削力以改善樣本品質。
有文獻指出凸面針比凹面針更適合作旋轉切削，但目前凸面針在組織切片的應用上仍較少見，在過去研究中，對於此類型曲面針也未被深入探討。本研究針對凸面針的幾何設計與切削參數進行切削力與樣本品質最佳化，利用田口方法設計軟組織切削與取樣實驗，分析各因子之影響力以及切削力與樣本品質之間的關聯性。
本研究主要發現為加入針尖導角、提高轉速比、提高K值及降低穿刺速度皆有助於降低切削力。高轉速比或高穿刺速度能取得較多樣本量，但隨之提高轉速導致樣本破碎。建議參數配置策略為使用K值0.2以上的凸面針、提高轉速比與降低前進速度。本研究所得之最佳參數為K值0.2、前進速度1 mm/s、轉速比8，能取得最多樣本總量並同時降低樣本破碎情形。

Vacuum-assisted biopsy is a commonly used minimally invasive technique that uses rotational cutting as the main sampling method. An accurate diagnosis of disease requires samples with good quality, which is affected by the cutting force of the biopsy needle. A low cutting force allows a needle to retrieve larger and non-crushed samples.
Studies have indicated that a needle with a convex-curved cutting edge is more suitable for rotational cutting than that with a concave-curved cutting edge. However, such a geometric design is not seen in the existing biopsy applications and is little investigated in the previous studies. This paper proposed a novel needle design with double convex-curved cutting edges. The aim is to optimize the design to extract large, non-crushed samples with minimal cutting force.
The Taguchi method was used for the design of experiments with five factors. The rotational needle insertion experiment and tissue sampling experiment were established for studying the effects of the factors on the cutting force and sampling quality, respectively. The correlation between the cutting force and the sampling quality was investigated.
The results showed that increasing K value or increasing rotation-translation ratio could significantly lower the cutting force. A larger sample could be extracted at a high rotation-translation ratio or a high insertion speed, but sample fragmentation could occur under high rotation speed. To improve sampling quality and cutting force simultaneously, the recommended strategy is to configure the cutting parameters with a higher K value, a larger rotation-translation ratio, and a smaller insertion speed. For the cases tested in this study, the optimal configuration of a needle would have a sharpened cutting edge with the K value at 0.2, rotation-translation ratio at 8, and insertion speed at 1 mm/s.

[1] E.A.M. O'Flynn, A.R.M. Wilson, and M.J. Michell, "Image-guided breast biopsy: state-of-the-art," Clinical Radiology, vol. 65, no. 4, pp. 259-270, 2010.
[2] W.B.G Sanderink and R.M. Mann, "Advances in breast intervention: where are we now and where should we be?," Clinical Radiology, vol. 73, no. 8, pp. 724-734, 2018.
[3] Mariebl D. Lacambra, Christopher C. Lam, Paulo Mendoza, Siu Ki Chan, Alex M. Yu, Julia Y. S. Tsang, Puay Hoon Tan, and Gary M. Tse, "Biopsy sampling of breast lesions: comparison of core needle-and vacuum-assisted breast biopsies," Breast Cancer Research and Treatment, vol. 132, no. 3, pp. 917-923, 2012.
[4] N. Abolhassani, R. Patel, and M. Moallem, "Needle insertion into soft tissue: A survey," Medical Engineering & Physics, vol. 29, no. 4, pp. 413-431, 2007.
[5] Pei-Ying Wu, H. Kahraman, and H. Yamaguchi, "Development of aspiration-assisted end-cut coaxial biopsy needles," Journal of Medical Devices, vol. 11, no. 1, pp. 011012, 2017.
[6] M A Meltsner, N J Ferrier, and B R Thomadsen, "Observations on rotating needle insertions using a brachytherapy robot," Physics in Medicine & Biology, vol. 52, no. 19, pp. 6027-6037, 2007.
[7] Tarun Podder, Lydia Liao, J. Sherman, Duane Fuller, Vladimir Misic, D. J. Ruben, E. M. Messing, J. G. Strang, Wing Sum Ng, and Yanlin Yu, "A method to minimize puncturing force and organ deformation," International Journal of Computer Assisted Radiology and Surgury, vol. 1, pp. 277-280, 2006.
[8] Peidong Han and Kornel Ehmann, "Study of the effect of cannula rotation on tissue cutting for needle biopsy," Medical Engineering & Physics, vol. 35, no. 11, pp. 1584-1590, 2013.
[9] J. Z. Moore, P. W. McLaughlin, and A. J. Shih, "Novel needle cutting edge geometry for end‐cut biopsy," Medical Physics, vol. 39, no. 1, pp. 99-108, 2012.
[10] A. G. Atkins, X. Xu, and G. Jeronimidis, "Cutting, by ‘pressing and slicing,’ of thin floppy slices of materials illustrated by experiments on cheddar cheese and salami," Journal of Materials Science, vol. 39, no. 8, pp. 2761-2766, 2004.
[11] Marco Giovannini, Huaqing Ren, Jian Cao, and Kornel Ehmann, "Study on design and cutting parameters of rotating needles for core biopsy," Journal of the Mechanical Behavior of Biomedical Materials, vol. 86, pp. 43-54, 2018.
[12] R. Tsumura, Y. Takishita, Y. Fukushima, and H. Iwata (2016, August), "Histological evaluation of tissue damage caused by rotational needle insertion," Paper presented at the 38th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC), pp. 5120-5123.
[13] M. N. Hochman and M. J. Friedman, "In vitro study of needle deflection: A linear insertion technique versus a bidirectional rotation insertion technique," Quintessence International, vol. 31, no. 1, pp. 33-39, 2000.
[14] M. N. Hochman and M. J. Friedman, "An in vitro study of needle force penetration comparing a standard linear insertion to the new bidirectional rotation insertion technique," Quintessence International, vol. 32, no. 10, pp. 789-796, 2001.
[15] Thomas R. Cox and Janine T. Erler, "Remodeling and homeostasis of the extracellular matrix: implications for fibrotic diseases and cancer," Disease Models & Mechanisms, vol. 4, no. 2, pp. 165-178, 2011.
[16] Abbas Samani, Judit Zubovits, and Donald Plewes, "Elastic moduli of normal and pathological human breast tissues: an inversion-technique-based investigation of 169 samples," Physics in Medicine and Biology, vol. 52, no. 6, pp. 1565-1576, 2007.
[17] Amit Gefen and Benny Dilmoney, "Mechanics of the normal woman's breast," Technology and Health Care, vol. 15, no. 4, pp. 259-271, 2007.
[18] Nilza G. Ramião, Pedro S. Martins, Rita Rynkevic, António A. Fernandes, Maria Barroso, and Diana C. Santos, "Biomechanical properties of breast tissue, a state-of-the-art review," Biomechanics and Modeling in Mechanobiology, vol. 15, no. 5, pp. 1307-1323, 2016.
[19] A.E. Forte, F. D'Amico, M.N. Charalambides, D. Dini, and J.G. Williams, "Modelling and experimental characterisation of the rate dependent fracture properties of gelatine gels," Food Hydrocolloids, vol. 46, pp. 180-190, 2015.
[20] Niki Abolhassani, Rajni Patel, Mehrdad Moallem, "Control of soft tissue deformation during robotic needle insertion," Minimally Invasive Therapy & Allied Technologies, vol. 15, no. 3, pp. 165-176, 2006.
[21] Andrew C. Barnett, Yuan-Shin Lee, and Jason Z. Moore, "Needle geometry effect on vibration tissue cutting," Proceedings of the Institution of Mechanical Engineers, Part B: Journal of Engineering Manufacture, vol. 232, no. 5, pp. 827-837, 2018.
[22] T. Chanthasopeephan, J. P. Desai, and Alan C.W. Lau (2006, February), "Determining fracture characteristics in scalpel cutting of soft tissue," Paper presented at the First IEEE/RAS-EMBS International Conference on Biomedical Robotics and Biomechatronics, 2006. BioRob 2006., pp. 899-904.
[23] Heike Preibsch, Astrid Baur, Beate M. Wietek, Bernhard Krämer, Annette Staebler, Claus D. Claussen, and Katja C. Siegmann-Luz, "Vacuum-assisted breast biopsy with 7-gauge, 8-gauge, 9-gauge, 10-gauge, and 11-gauge needles: how many specimens are necessary?," Acta Radiologica, vol. 56, no. 9, pp. 1078-1084, 2015.
[24] M. Ramazanilar and A. Mojra, "Characterization of breast tissue permeability for detection of vascular breast tumors: An in vitro study," Materials Science and Engineering: C, vol. 107, pp. 110222, 2020.
[25] H. Kataoka, T. Washio, K. Chinzei, K. Mizuhara, C. Simone, and A. M. Okamura (2002, October), "Measurement of the tip and friction force acting on a needle during penetration," Paper presented at International Conference on Medical Image Computing and Computer-Assisted Intervention, pp. 216-223.
[26] A. M. Okamura, C. Simone, and M. D. O'leary, "Force modeling for needle insertion into soft tissue," IEEE Transactions on Biomedical Engineering, vol. 51, no. 10, pp. 1707-1716, 2004.
[27] M. Oldfield, D. Dini, G. Giordano, and F. Rodriguez y. Baena, "Detailed finite element modelling of deep needle insertions into a soft tissue phantom using a cohesive approach," Computer Methods in Biomechanics and Biomedical Engineering, vol. 16, no. 5, pp. 530-543, 2013.
[28] Yancheng Wang, Bruce L. Tai, Roland K. Chen, and Albert J. Shih, "The needle with lancet point: geometry for needle tip grinding and tissue insertion force," Journal of Manufacturing Science and Engineering, vol. 135, no. 4, pp. 041010, 2013.
[29] Bruce L. Tai, Yancheng Wang, and Albert J. Shih (2013, June), "Cutting force of hollow needle insertion in soft tissue," Paper presented at ASME 2013 International Manufacturing Science and Engineering Conference.
[30] Yancheng Wang, Roland K. Chen, Bruce L. Tai, Patrick W. McLaughlin, and Albert J. Shih, "Optimal needle design for minimal insertion force and bevel length," Medical Engineering & Physics, vol. 36, no. 9, pp. 1093-1100, 2014.
[31] Marco Giovannini, Huaqing Ren, Xingsheng Wang, and Kornel Ehmann, "Tissue cutting with microserrated biopsy punches," Journal of Micro and Nano-Manufacturing, vol. 5, no. 4, pp. 041004, 2017.
[32] Niki Abolhassani, Rajni Patel, and Mehrdad Moallem, "Experimental study of robotic needle insertion in soft tissue," International Congress Series, vol. 1268, pp. 797-802, 2004.
[33] Yancheng Wang, Weisi Li, Peidong Han, Marco Giovannini, Kornel Ehmann, and Albert J. Shih, "Contributions in medical needle technologies—geometry, mechanics, design, and manufacturing," Machining Science and Technology, vol. 20, no. 1, pp. 1-43, 2016.
[34] Jason Z. Moore, Qinhe Zhang, Carl S. McGill, Haojun Zheng, Patrick W. McLaughlin, and Albert J. Shih, "Modeling of the plane needle cutting edge rake and inclination angles for biopsy," Journal of Manufacturing Science and Engineering, vol. 132, no. 5, pp. 051005, 2010.
[35] Jason Z. Moore, Qinhe Zhang, Carl S. McGill, Haojun Zheng, Patrick W. McLaughlin, and Albert J. Shih, "Modeling cutting edge geometry for plane and curved needle tips," Proceedings of the Institution of Mechanical Engineers, Part B: Journal of Engineering Manufacture, vol. 226, no. 5, pp. 861-869, 2012.
[36] Yingqiang Xu, Xuemei Qin, Guowei Liu, Lei Tan, Hongjian Dong, Pengpeng Wei, Qinhe Zhang, and Hongcai Zhang, "A new method for evaluating the normal rake angle and inclination angle on medical needles," Proceedings of the Institution of Mechanical Engineers, Part H: Journal of Engineering in Medicine, vol. 232, no. 1, pp. 24-32, 2018.
[37] Marco Giovannini, Jian Cao, and Kornel Ehmann, "Design and models of helical needle geometries for core biopsies," Journal of the Mechanical Behavior of Biomedical Materials, vol. 90, pp. 113-124, 2019.
[38] T.K. Podder, D.P. Clark, D. Fuller, J. Sherman, W.S. Ng, L. Liao, D.J. Rubens, J.G. Strang, E.M. Messing, Y.D. Zhang, and Y.Yu (2005, September), "Effects of velocity modulation during surgical needle insertion," Paper presented at the 2005 IEEE Engineering in Medicine and Biology 27th Annual Conference, pp. 5766-5770.
[39] Mohsen Mahvash and Pierre E. Dupont (2009, May), "Fast needle insertion to minimize tissue deformation and damage," Paper presented at 2009 IEEE International Conference on Robotics and Automation, pp. 3097-3102.
[40] Helene M. Langevin, David L. Churchill, James R. Fox, Gary J. Badger, Brian S. Garra, and Martin H. Krag, "Biomechanical response to acupuncture needling in humans," Journal of Applied Physiology, vol. 91, no. 6, pp. 2471-2478, 2001.
[41] Berndt-Michael Order, Philipp J. Schaefer, G. Peter, Christel Eckmann-Scholz, Felix Hilpert, Alexander Strauss, Viktoria Warneke, Micaela Mathiak, Martin Heller, Walter Jonat, and Fritz K.W. Schaefer, "Evaluation of two different vacuum-assisted breast biopsy systems: Mammotome® system 11G/8G vs. ATEC® system 12G/9G," Acta Radiologica, vol. 54, no. 2, pp. 137-143, 2013.
[42] Kenneth D. Hopper, Catherine S. Abendroth, Kraig W. Sturtz, Yvonne L. Matthews, Laurie A. Stevens, and Susan J. Shirk, "Automated biopsy devices: a blinded evaluation," Radiology, vol. 187, no. 3, pp. 653-660, 1993.
[43] Alexander Poellinger, Ulrich Bick, Torsten Freund, Susanne Diekmann, Bernd Hamm, and Felix Diekmann, "Evaluation of 11-gauge and 9-gauge vacuum-assisted breast biopsy systems in a breast parenchymal model," Academic Radiology, vol. 14, no. 6, pp. 677-684, 2007.
[44] M. Hahn et al., "Vacuum-assisted breast biopsy: a comparison of 11-gauge and 8-gauge needles in benign breast disease," World Journal of Surgical Oncology, vol. 6, no. 1, pp. 51, 2008.
[45] Shambhavi Venkataraman, Vandana Dialani, Hannah L. Gilmore, and Tejas S. Mehta, "Stereotactic core biopsy: comparison of 11 gauge with 8 gauge vacuum assisted breast biopsy," European Journal of Radiology, vol. 81, no. 10, pp. 2613-2619, 2012.
[46] Ming Zhang, Y. P. Zheng, and Arthur F. T. Mak, "Estimating the effective Young's modulus of soft tissues from indentation tests—nonlinear finite element analysis of effects of friction and large deformation," Medical Engineering & Physics, vol. 19, no. 6, pp. 512-517, 1997.