分子模版高分子 (Molecularly imprinted polymer, MIP) 主要是將模版分子 (template) 、具有官能基的高分子單體和高比例的交聯劑，利用高度交聯聚合結果，將模版分子包埋在高分子結構中，高分子基材因此形成形狀大小和官能基與模版分子互補的辨識結構，對模版分子具有良好的辨識能力和選擇性，而且高分子材料擁有耐高溫、酸鹼和可在有機溶劑下使用的特點，因此分子模版高分子可以作為感測器的辨識元件。
本實驗以膽紅素 (bilirubin) 當作模版分子、MAA為單體、EGDMA為交聯劑，以熱聚合的方式製備模版高分子顆粒，以掃瞄式電子顯微鏡和IR圖譜探討膽紅素模版高分子的各項物理特性，藉此鑑定膽紅素模版高分子之製備成功與否，以及其吸附和脫附之結果，藉由分別比較膽紅素模版高分子對相似物和血清中共存物質與膽紅素的吸附能力探討選擇性。以UV光聚合的方式製備模版高分子薄膜，結合石英微量晶體天秤 (quartz crystal microbalance, QCM) 的感測方式探討膽紅素模版高分子薄膜吸附的情形。

Molecularly imprinted polymer (MIP) was prepared by mixing the functional monomer and the target template, biliruibin, with a high ratio of crosslinker. Bulk polymerization was carried out so that the polymer with a high degree of crosslinking was obtained. In this way, the template molecule, bilirubin was entrapped within the polymer matrix. The recognition sites’ shape and functional groups should be complementary to the templates. Hence, the polymer thus prepared could have good recognition and selection for the templates. In addition, the polymer could have the tolerance for operating at high temperatures, either acidic or basic, as well as organic solvent conditions. In this research, the MIP was applied to establish a sensing system for the detection of bilirubin.
During the synthesis of MIP, bilirubin was used as the target template, MAA was the functional monomer and EGDMA was chosen as the crosslinker. Thermal polymerization was chosen to investigate the features of the MIP as well as the desorption and binding effect of the polymer while UV irradiation polymerization was chosen for the preparation of the MIP thin film. SEM and IR were applied to examine and identify the MIP being prepared. The binding specificity of the bilirubin imprinted polymer was also discussed by using the bilirubin analog and the compounds co-existing in the serum. Quartz crystal microbalance (QCM) was also used as the transducer to detect the signal caused by the adsorption of biliruibin onto the MIP film.

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