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研究生：	孫偉倫 Sun, Wei-Lun
論文名稱：	含有小分子的拉曼標記之組裝及分析 Assembly and Characterization of Raman Tags with small molecules
指導教授：	陳宣燁 Chen, Shiuan-Yeh
學位類別：	碩士 Master
系所名稱：	理學院 - 光電科學與工程學系 Department of Photonics
論文出版年：	2019
畢業學年度：	107
語文別：	中文
論文頁數：	58
中文關鍵詞：	表面增強拉曼散射、拉曼標記、二氧化矽殼、核心-衛星組裝
外文關鍵詞：	SERS, Raman tag, silica shell, core-satellite assemblies
相關次數：	點閱：74 下載：3
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近年來，金屬奈米粒子的應用已有相當廣泛的研究，如表面增強拉曼散射(surface-enhanced Raman scattering)、表面增強螢光(surface enhanced fluorescence)局域性表面電漿共振(Localized surface plasmon resonance)，通常會被應用在分子訊號增強、分子感測與生醫成像，其中有很多研究利用金屬表面的不連續處或粒子間隙中的高電場增強來提升金屬表面分子的訊號強度[4][5][6][7][8][11]。而拉曼標記則是其中一種，其優點在於分子拉曼訊號的高穩定度與高辨識性，分子的訊號則是利用結構內的高電場增強。本論文中利用靜電吸附形成的核心－衛星奈米金屬架構，將BDT、DMcT或Cy5作為拉曼分子。合成後的核心－衛星奈米金屬架構將會包覆二氧化矽殼，一方面避免結構遭受環境破壞，另一方面也賦予其修飾上抗體的可行性。
在本論文中除了提供一個可行的製程製作可進行定量分析的金屬叢集架構，另一方面也透過二氧化矽殼製程的優化與改良複合核心-衛星組裝粒子的方法進行分散性、衛星吸附量的優化，整個製程的製作時間可在一天之內完成。具有高分散性的粒子較容易進行定量分析量測且有利於後續的抗體修飾，衛星粒子吸附量的提升則會直接影響拉曼訊號的強度。
最後將會比較相同的核心－衛星奈米金屬架構下含BDT及Cy5的拉曼標記與含Cy5的PS粒子的亮度比較，了解拉曼訊號相較於螢光分子訊號的亮度之差距以及應用上的可行性。

In recent years, nano-metal materials have been extensively researched, including surface-enhanced Raman scattering, surface enhanced fluorescence and Localized surface plasmon resonance. They are usually applied in signal enhancement, molecular detection and biomedical image. Many kinds of research take advantage of discontinuous surface or nano-gap on metal to enhance the scattering signal. Raman tag is one of those applications to solve the problem that the intensity of the Raman signal is too weak to apply. The benefits of Raman tag are high identification, sensitivity and stability.

In this report, we embed Raman reporter in the core-satellite gold nanostructure that has been developed and optimized the process of silica coating and the adsorbed number of satellite particles. All the production could be finished in one day. In optical measurement, we could understand an average intensity at characteristic peaks in the Raman spectra for Raman tag, and the comparison between Fluorescent molecules and Raman tag embedded by Cy5 and 1,4-Benzenedithiol (BDT).

摘要...........................................I
ABSTRACT......................................II
致謝.........................................VIII
目錄...........................................IV
圖目錄.........................................VI
表目錄.......................................VIII
第一章 序論.........................................1
1-1 前言..........................................1
1-2 研究動機與目的.................................3
1-3 文獻回顧......................................3
第二章 方法.......................................8
2.1  實驗儀器原理...................................8
2.1.1 紫外-可見光吸收光譜儀........................8
2.1.2穿透式電子顯微鏡..............................8
2.1.3動態光散射儀(Dynamic light scattering).......10
2.1.4拉曼光譜儀...................................11
2.1.5彈性與非彈性散射系統..........................13
2.2  拉曼散射、表面增強拉曼散射與表面電漿共振........15
2.2.1  拉曼散射...................................15
2.2.2  表面增強拉曼散射............................16
2.2.3  表面電漿共振................................16
2-3二氧化矽殼反應機制..............................17
2-4 複合粒子包二氧化矽殼製程與量測設置..............18
2-4-1複合粒子製程.................................18
2-4-2二氧化矽殼製程...............................20
2-4-3補充各藥品配置方法...........................21
2-4-4補充實驗室現有的複合粒子製程..................23
2-4-5 拉曼光譜量測流程............................23
2.5 縮寫與簡稱的解釋列表..........................24
第三章 實驗結果與討論...............................25
3-1 加入DMCT的複合粒子修飾........................25
3-1-1 DMcT在金粒子表面上的修飾....................25
3-1-2 修飾聚電解質PAH............................26
3-1-3 製作複合粒子CSA(56 μM DMcT+1.2 mM PAH).....27
3-1-4 對PAH的離心參數優化........................29
3-1-5  DMcT反應濃度的調整與離心步驟的取捨.........30
3-2 採用BDT為標記分子的複合粒子製程...............33
3-2-1 BDT分子修飾金表面..........................33
3-2-2 BDT、PAH離心參數與PAH分子的修飾.............34
3-3 不同BDT反應濃度對於複合粒子的影響..............36
3-4 二氧化矽殼製程的優化..........................37
3-4-1 複合粒子包二氧化矽殼製程之聚集程度初步改善....38
3-4-2 TEOS反應濃度提升對製程的可行性...............40
3-5優化複合粒子的衛星吸附量........................44
3-6 第一次定量分析量測結果.........................46
3-7 第二次定量分析量測結果.........................52
第四章 結論與討論...................................54
4-1實驗成果總結...................................54
4-2改進方向.......................................55
參考文獻..........................................56
                                    

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校內：2021-09-01公開
校外：2021-09-01公開

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