磁性奈米粒子的發展非常迅速，並且已廣泛應用於生物醫學領域。其獨特的超順磁特性，提供了非侵入式與非接觸式的特質，亦可藉由外加磁場的調控，將其作為體內傳遞藥物的載體，再配合專一辨識蛋白的連結以及熱治療，有效精準的抑制、甚至破壞癌細胞。
本研究利用水相共沉澱法製備磁性奈米粒子，在其外層修飾環糊精 (β-cyclodextrin, β-CD) 以及運鐵蛋白 (transferring, Tf)，利用環糊精疏水性的中軸空腔吸附、裝載抗癌藥物；而運鐵蛋白則是作為靶向配體，達到藥物傳遞以及腫瘤標靶之雙重功效，最後探討奈米磁球的熱效應。研究過程中以 X-ray繞射儀鑑定磁性奈米粒子的晶相以及估算其平均微晶尺寸；以傅立葉紅外線光譜儀 (Fourier Transform Infrared Spectroscopy, FTIR) 與紫外光/可見光光譜儀 (UV/Vis Spectrophotometer) 判斷β-CD及 transferrin分別是否修飾在 Fe3O4表面；以穿透式顯微鏡 (Transmission Electron Microscope, TEM) 分析觀察 Fe3O4、Fe3O4@β-CD與 Fe3O4@β-CD-Tf 的影像變化以及估算平均粒徑，分別為9.01 ± 1.98 nm、10.23 ± 1.62 nm與14.08 ± 2.30 nm。在磁性分析上，以超導量子干涉儀 (Superconducting Quantum Interference Device, SQUID) 量測磁滯曲線得Fe3O4、Fe3O4@β-CD與 Fe3O4@β-CD-Tf之飽和磁化量分別為69.99 emu/g、65.32 emu/g與55.62 emu/g，並且三者皆具有超順磁的性質，顯示在超順磁材料上披覆不同有機物質並不影響其磁性質。
在醫療應用上，則是將奈米磁球作滅菌處理，探討滅菌後的磁球於檢測中是否產生變化。以具有抑制癌細胞效果之 capsaicin進行藥物包覆及釋放探討，奈米磁球的藥物釋放最高效率可維持在七小時以上。於熱效應中，將Fe3O4、Fe3O4@β-CD與 Fe3O4@β-CD-Tf放置於高頻電磁波中，可觀察到在10分鐘內奈米磁球分別可升溫至84 oC、60 oC及55 oC，顯示經過修飾後的奈米磁球之熱效應雖然明顯下降，但是依然具有良好之抗熱效果，因此初步呈現未來進一步熱治療施行之可行性。

Research on the development of superparamagnetic nanoparticles for biomedical purposes has been widely paid attention in recent years. Due to the superparamagnetic behavior, the nanoparticles can be applied to not only non-invasive monitoring but also site-specific targeting upon an external magnetic field. Additionally, conjugation of the recognition protein onto the surfaces of the nanoparticle provides the nanocarriers targeting ability. Via which, hyperthermia treatment can directly inhibit or even kill the carcinoma.
In this study, the superparamagnetic nanoparticles were synthesized by co-precipitation. The surface of magnetic nanoparticles (MNP) was further modified with β-cyclodextrin (β-CD) and transferring (Tf). Cyclodextrin is able to form inclusion complexes with many drugs by taking up the drug molecule into the central cavity due to its capability of being a good host for the inclusion of guest molecule. While transferrin can be used to detect the cancer cells. Combining these two species, the magnetic nanoparticles can be designed with dual functionality as drug carriers and targeting effectiveness. And the thermal effect of the iron oxide nanoparticles under applied magnetic field was also investigated. XRD was used to characterize the stretch bonds of Fe3O4 magnetic nanoparticles. Modification by β-CD and β-CD-transferrin to Fe3O4 were confirmed by FT-IR and UV/Vis spectrophotometer. The size of Fe3O4 、Fe3O4@β-CD and Fe3O4@β-CD-Tf were estimated to be 9.01 ± 1.98 nm、 10.23 ±1.62 nm and 14.08 ± 2.30 nm, respectively, by TEM. SQUID results indicated that saturation magnetization of Fe3O4, Fe3O4@β-CD, and Fe3O4@β-CD-Tf.
For biomedical applications, the MNPs must be sterilized before use. The loading and releasing efficiency of capsaicin was investigated. Capsaicin was reported to be able to inhibit the cancer cells. The maximum loading ability of MNP was 14.72 %. Capsaicin can be within 7 hours. Under induced electro-magnetic field, the temperature of Fe3O4, Fe3O4@β-CD, and Fe3O4@β-CD-Tf could increase up to 84 oC, 60 oC, and 55 oC in 10 minutes, respectively. Consequently, it has shown that the MNPs have the feasibility toward hyperthermia therapy.

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