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研究生： 劉姿蘭
Liu, Tzu-Lan
論文名稱： 合成膽固醇分子模版之探討
The Investigation on the Optimal Composition for Synthesizing Cholesterol-Imprinted Polymers
指導教授： 葉茂榮
Yeh, Mou-Yung
學位類別： 碩士
Master
系所名稱： 理學院 - 化學系碩士在職專班
Department of Chemistry (on the job class)
論文出版年： 2006
畢業學年度： 94
語文別： 中文
論文頁數： 186
中文關鍵詞： 膽固醇 、模版
外文關鍵詞： cholesterol, imprinted
相關次數： 點閱：133  下載：0 
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    • 臨床上檢驗血液中膽固醇濃度可以協助醫生診斷心臟血管疾病。 目前在臨床上，以酵素法為血液膽固醇檢驗為最常被使用的方法，主要是因為此方法具有較佳之選擇性、靈敏性及準確性，然而，此方法仍是以呈色反應來定量濃度，故受測樣品仍需經過適當的前處理，才能減少血液中存在的干擾物質所造成的影響，並需足夠的反應時間。而利用分子模板之方法，可以增加偵測的選擇性及專一性，提高準確性。
    利用微卡計得到最佳的官能基( MAA )與交聯劑( EGDMA )，且官能基( MAA )與膽固醇最佳莫耳比為1，也由吸附效能測試得到證實MAA/ CHO莫耳比為1時α=2.45，MAA/CHO莫耳比為3時α=1.5。MAA/ CHO莫耳比為1以共價( cme-MIP )及非共價鍵結( c-MIP )形成膜版，經過一系列膽固醇吸附測試與競爭性吸附，數據都顯示cme-MIP吸附及選擇性優於c-MIP。cme-MIP最大吸附量1.93mg / 0.3g polymer。物理測試上cme-MIP的孔洞平均，剛性較強，IR顯示吸附較完全。c-MIP的孔洞不平均，剛性較弱，IR吸附較不完全，種種結果顯示，膽固醇膜版以共價鍵結比非共價鍵結要來好。

    The examination of the cholesterol concentrations in blood is an important index for doctors to diagnose the heart blood vessel disease of patients clinically. The enzyme method is the most popular way to analyze the cholesterol concentration in blood, since it has a better selectivity, sensitivity and accuracy. However, the quantification of this method is based on color variation; samples must be pre-treated to reduce the interferences existing in blood. Additionally, the reaction time is long. The got optimal function and crosslinking monomer that mole ratio 1 by microcalarmeter. The imprinting effect MAA/CHO mole ratio 1, α=2.45, MAA/CHO mole ratio 3, α=1.5. The mole ratio 1 make molecular imprinting by covalent ( cme-MIP ) and non-covalent ( c-MIP ), that using cholesterol solution and competition solution with stigmesterol or progestone as adsorbed solution. The results show cme-MIP imprinting effect and selectivity better than c-MIP. The c-MIP got maximum adsorbed amount 1.93mg / 0.3 g polymer. The physics test that cme-MIP better that have imprinting same size hole, rigidity and adsorbed full that c-MIP. For these reason making cholesterol -imprinting by covalent better than non-covalent.

    中文摘要 Ⅱ 英文摘要 Ⅲ 致謝 Ⅳ 目錄 Ⅴ 表目錄 Ⅸ 圖目錄 Ⅹ 專有名詞對照表 ⅩⅦ 第一章 緒 論 1 1-1膽固醇的探討 1 1-2肝臟膽固醇來源 4 1-3膽固醇的合成 6 1-4 膽固醇濃度的調節 9 1-5膽固醇的轉化 9 1-6高膽固醇所造成的血脂肪與動脈硬化 10 1-7低膽固醇所造成的疾病 13 1-8膽固醇酯酵素 15 1-9影響膽固醇氧化因子 16 1-10膽固醇氧化產物簡介 19 第二章 原 理 20 2-1分子模板原理 20 2-1-1 分子模板的製作方式 23 2-1-2 溶劑 25 2-1-3 溫度 26 2-2分子模板材料選擇 30 2-2-1吸附效能 32 2-2-2目前一般在選用官能基單體有三類 33 2-2-3一般常用的交連劑單體 34 2-2-4一般常用的起始劑單體 36 2-3分子模板高分子知化學與溫度穩定性 36 2-4分子模板高分子的偵測 37 2-4-1 電導感測器 37 2-4-2 螢光偵測系統 38 2-5分子模板應用 39 第三章 實 驗 動 機 41 3-1膽固醇強酸發色法 41 3-2膽固醇酵素法 41 3-3膽固醇電化學法 43 3-4膽固醇分子模板感測法 43 3-4-1非共價鍵結 44 3-4-2共價鍵結方式 44 3-5本論文實驗原理 47 3-5-1第一種方法：結合共價與非共價鍵結法 47 3-5-2第二種方法：使用非共價鍵結法 48 3-6利用檢測儀器來測定所製作膽固醇分子模板性質 49 3-6-1核磁共振 49 3-6-2熱重分析儀 52 3-6-3紅外線光譜 54 3-6-4高效能液相層析 58 3-6-5微卡計 60 3-6-6 氮氣等溫吸附脫附測量 66 第四章 實 驗 設 備 與 方 法 71 4-1藥品與儀器 71 4-1-1藥品 71 4-1-2儀器 74 4-2實驗方法 75 4-2-1膽固醇酯之合成 75 4-2-2微熱卡計之等溫滴定 76 4-2-3分子模板粉粒體製備 79 4-2-3-1製備方法一，膽固醇分子模板 79 4-2-3-2製備方法二，膽固醇甲基丙烯酸酯分子模板 80 4-2-3-3製備方法三，非膽固醇分子模板 81 4-2-4模板吸附分析 83 4-2-5HPLC操作與前處理 84 4-2-6膽固醇與膽固醇甲基丙烯酸酯相對於溶劑最少使用量 84 4-2-7微分掃瞄卡計 85 4-2-8 傅利葉轉換紅外線光譜儀 85 4-2-9 熱重分析儀 85 4-3實驗架構 86 第五章 結 果 與 討 論 87 5-1合成膽固醇甲基丙烯酸酯的結構鑑定 87 5-1-1核磁共振光譜儀C譜圖 88 5-1-2核磁共振光譜儀H譜圖 89 5-1-3紅外光譜圖 90 5-1-4氣相層析質譜圖 91 5-2微卡計的等溫滴定 92 5-2-1單體與單體或單體與目標物之間的關係 94 5-2-1-1功能性單體滴定膽固醇粉體 94 5-2-1-2交聯劑單體滴定膽固醇粉體 105 5-2-1-3功能性單體滴定交聯劑單體 117 5-2-1-4功能性單體滴定膽固醇溶液 120 5-2-2膽固醇溶液滴定分子模板 126 5-2-2-1膽固醇溶液滴定cme-MIP 128 5-2-2-2膽固醇溶液滴定c-MIP 133 5-2-2-3膽固醇溶液滴定NIP 138 5-3分子模板粉粒體測試 143 5-3-1HPLC分析膽固醇之穩定度 145 5-3-2膽固醇溶液在HPLC的校正曲線 146 5-3-3荳固醇溶液在HPLC的校正曲線 147 5-3-4類固醇溶液在HPLC的校正曲線 148 5-3-5膽固醇對分子模板吸附比較 149 5-4物性測試 158 5-4-1膽固醇對各種溶劑之溶解度 158 5-4-2膽固醇酯模板分子清洗下來核磁共振光譜儀H譜圖 161 5-4-3以紅外光譜測量分子模板清洗前與清洗後比較圖 162 5-4-4以熱重分析儀量測清洗前與清洗後比較 166 5-4-5以BET測量孔徑大小 169 第六章 結 論 與 建 議 173 參考文獻 175

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