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研究生: 張彗庭
Chang, Hui-Ting
論文名稱: TRAF6與IRAK1蛋白質複合物的結構及生化分析
Structural and biochemical studies of the TRAF6 and IRAK1 protein complex
指導教授: 羅玉枝
Lo, Yu-Chih
學位類別: 碩士
Master
系所名稱: 生物科學與科技學院 - 生物科技與產業科學系
Department of Biotechnology and Bioindustry Sciences
論文出版年: 2023
畢業學年度: 111
語文別: 英文
論文頁數: 88
中文關鍵詞: TRAF6IRAK1 TLRs/IL-1Rs訊息傳遞路徑
外文關鍵詞: TRAF6, IRAK1, TLRs/IL-1Rs signaling pathway
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  • Chinese Abstract (中文摘要) I Abstract II Acknowledgments VI Table of Contents VII Contents of Tables IX Contents of Figures X Contents of Appendices XI Abbreviation List XII 1. Research Background 1 1-1 Innate immune response through TLRs/IL-1Rs signaling pathways 1 1-2 TRAF6 1 1-3 IRAK1 4 1-4 The aims of this study 5 2. Materials and Methods 7 2-1 Protein expression constructs 7 2-2 Recombinant protein expression and purification 7 2-3 X-ray crystallography 8 2-4 SAXS 9 2-5 MALS 9 3. Results 10 3-1 Construction of TRAF6 and IRAK1 recombinant plasmids 10 3-2 Expression and purification of recombinant proteins 10 3-3 Optimization of protein expression and purification conditions 11 3-4 Co-purification of TRAF6 and IRAK1 to form protein complex 12 3-5 Solve the protein structure through X-ray crystallography 14 3-6 SAXS data collection 16 3-7 Multiangle light scattering (MALS) 17 3-8 Pull-down assay 18 3-9 SEC co-purification to reconfirm the conclusions of the pull-down assay 19 4. Discussion 21 References 27 Tables 33 Figures 39 Appendices 82

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